Determination of estrogens and their conjugates in water using solid-phase extraction followed by liquid chromatography–tandem mass spectrometry

Isobe, Takeshi; Shiraishi, Hiroaki; Yasuda, Masaru; Shinoda, Akiko; Suzuki, Hiroshi; Morita, Masatoshi

doi:10.1016/s0021-9673(02)01851-4

Cited by 217 publications

(155 citation statements)

References 25 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…Therefore, the pH condition was optimized from pH 3 to pH 4. Previous studies also showed that better retention could be acquired by acidified water sample (Ternes et al 1999;Isobe et al 2003;Quintana et al 2004;Bila et al 2007).…”

Section: Resultsmentioning

confidence: 92%

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

Zhou

et al. 2008

Environ Monit Assess

View full text Add to dashboard Cite

A procedure using pre-column trimethylsilyl derivatization and gas chromatography/ mass spectrometry (GC/MS) was developed and applied in determining trace estrogens in complex matrix. Main conditions were optimized, including pH value, salinity of water sample, elution reagents, clean procedure, derivative solvent and temperature. The optimized method was used to determine steroid estrogens in surface water and effluents of wastewater treatment plant (WWTP). Low detection limits of 0.01, 0.03, 0.03, 0.07, 0.09 and 0.13 ng/l for DES, E1, E2, EE2, E3 and E V , respectively were obtained under optimism condition. No apparent interferences appeared in chromatography in comparison with ultrapure water blank. Mean recovery ranged from 72.6% to 111.0% with relative standard deviation of

show abstract

Section: Resultsmentioning

confidence: 92%

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

Zhou

et al. 2008

Environ Monit Assess

View full text Add to dashboard Cite

show abstract

“…In New Zealand, natural and the synthetic estrogens were also observed in animal wastes and sewage treatment plant effluents (Sarmah et al, 2006). In Japan, natural and synthetic substances having estrogenic activity were found in the Tokyo metropolitan area (Isobe et al, 2001, Isobe et al, 2003. Unfortunately, we do not have data of chemical analysis of the sampling sites.…”

Section: Discussionmentioning

confidence: 93%

Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet (Mugil cephalus)

Aoki

Nagae

Takao

et al. 2010

Science of The Total Environment

View full text Add to dashboard Cite

We monitored the contamination by environmental estrogens (EEs) of coastal areas in Korea and Japan using the wild grey mullet. The grey mullet were collected from Ansan, Jeju, Yeosu, Tongyeong, and Busan in Korea and Nagasaki, Omuta, and Fukuoka in Japan.Contamination by EEs was determined by measuring vitellogenin (VTG) levels in serum and identifying gonadal abnormalities histologically (i.e., testis-ova). In four sites in Korea (Ansan, Yeosu, Tongyeong, and Busan) and two sites in Japan (Nagasaki and Fukuoka), serum VTG in immature and male grey mullet was detected at levels greater than 1.0 g/ml, which is considered to be an abnormal level. Although, testis-ova were observed in some individuals collected in Ansan, Tongyeong, and Busan in Korea and Omuta in Japan, there was no correlation between individuals with testis-ova and individuals with abnormal levels of VTG. Furthermore, in Japan, serum VTG levels of fish collected from Nagasaki and Fukuoka was also greater than 1.0 g/ml. Although individuals with testis-ova were found in Omuta, these fish expressed normal levels of serum VTG. Our results suggest that the grey mullet living in these coastal areas are influenced by EEs in the environment. Furthermore, it appears that the production of VTG and the occurrence of testis-ova are caused by different mechanisms.

show abstract

“…Such an approach is not required when utilising LC/MS/MS, as further fragmenting the chosen ion to the daughter ions retains sensitivity and confirms quantification [24] . However, as sensitivity is less compromised with tandem MS, 2 ions may be used and then each further fragmented to produce daughter ions giving unequivocal confirmation [23] .…”

Section: Lc/ms Methods Developmentmentioning

confidence: 97%

“…Recently, extrapolation from the clinical field has utilised detection by LC tandem MS (LC/MS/MS) with electrospray ionisation (ESI) [23][24][25] for conjugates in environmental aqueous matrices. The use of LC/MS(/MS) has the following advantages, (i) Direct determination of the conjugate rather than a modified derivative ensures greater accuracy and allows moiety contribution and carbon positioning of the conjugate moiety to be deduced;…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of natural and synthetic steroid estrogens and their conjugates in aqueous matrices by liquid chromatography/mass spectrometry

Gomes¹,

Birkett²,

Scrimshaw³

et al. 2005

International Journal of Environmental Analytical Chemistry

View full text Add to dashboard Cite

An analytical method for the simultaneous determination of nine free and conjugated steroid estrogens was developed with application to environmental aqueous matrices. Solid phase extraction (SPE) was employed for isolation and concentration, with detection by liquid chromatography/mass spectrometry (LC/MS) using electrospray ionisation (ESI) in the negative mode. Method recoveries for various aqueous matrices (wastewater, lake and drinking water) were determined, recoveries proving to be sample dependent. When spiked at 50 ng/l concentrations in sewage influent, recoveries ranged from 62-89 % with relative standard deviations (RSD) < 8.1 %. In comparison, drinking water spiked at the same concentrations had recoveries between 82-100 % with an RSD < 5%. Ion suppression is a known phenomenon when using ESI; hence its impact on method recovery was elucidated for raw sewage. Both ion suppression from matrix interferences and the extraction procedure has bearing on the overall method recovery. Analysis of municipal raw sewage identified several of the analytes of interest at ng/l concentrations, estriol (E3) being the most abundant. Only one conjugate, estrone 3-sulphate (E1-3S) was observed.

show abstract

Determination of estrogens and their conjugates in water using solid-phase extraction followed by liquid chromatography–tandem mass spectrometry

Cited by 217 publications

References 25 publications

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry

Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet (Mugil cephalus)

Simultaneous determination of natural and synthetic steroid estrogens and their conjugates in aqueous matrices by liquid chromatography/mass spectrometry

Contact Info

Product

Resources

About