Determination of ethanol in blood by gas chromatography

The role of ␣ 1 -adrenoceptors in lipid mobilization and blood flow was investigated in situ using microdialysis of subcutaneous adipose tissue in severely obese subjects. The lipolysis rate was assessed by determination of interstitial glycerol concentration. The ␣ 1 -adrenoceptor agonist norfenefrine caused an increase in glycerol level in adipose tissue that was similar to that observed with the physiologic ␣ 1,2 -␤ 1 -adrenoceptor agonist norepinephrine, whereas the ␣ 1 -adrenoceptor antagonist urapidil showed no effect on basal lipolysis rate. However, the enhanced glycerol concentration due to norfenefrine and norepinephrine was suppressed in the presence of urapidil. The ␤-adrenoceptor antagonist propranolol showed no effect on norfenefrine-stimulated glycerol outflow. Blood flow was assessed using the ethanol escape technique. Perfusion with norfenefrine decreased blood flow, whereas urapidil enhanced blood flow significantly. Despite the increase in blood flow, the basal interstitial glycerol concentration remained unchanged. Although norfenefrine at high concentrations could inhibit the urapidil-induced increase in blood flow, the norfenefrine-induced glycerol output was not affected. These results demonstrate that ␣ 1 -adrenoceptors are involved in regulation of lipolysis rate and microcirculation of adipose tissue. However, the observed changes in local blood flow were not related to glycerol output.

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“…For ethanol measurement, two consecutive samples were combined. Ethanol concentrations were determined by gas-chromatography (Curry et al, 1966).…”

Section: Methodsmentioning

confidence: 99%

In Vivo α₁-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects

Flechtner-Mors

Jenkinson²,

Alt

et al. 2002

J Pharmacol Exp Ther

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“…Blood ethanol concentrations were measured by gas chromatography, according to the method of Curry et al (1966). The lower limit of sensitivity was 10 mg l-l.…”

Section: Drug Assaysmentioning

confidence: 99%

Comparison of the effects of therapeutic doses of meptazinol and a dextropropoxyphene/paracetamol mixture alone and in combination with ethanol on ventilatory function and saccadic eye movements.

Ali¹,

Marshall²,

Allen³

et al. 1985

Brit J Clinical Pharma

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CF4 4XN and 'Wyeth Research (UK), Taplow, Maidenhead, Berkshire, UK 1 The respiratory and psychomotor effects of a single oral dose of meptazinol (200 mg) and dextropropoxyphene (65 mg)/paracetamol (650 mg) mixture, was compared alone and in combination with ethanol (0.8 g kg-').2 Peak saccade velocity following meptazinol or the dextropropoxyphene/paracetamol mixture was not significantly different from placebo. When each of the treatments was followed by ethanol administration, a significant decrease in saccade velocity (P < 0.01) was seen. 3 Given alone, neither of the analgesic drugs produced a significant change in the slope of the ventilatory response to hypercapnia. Ethanol did not affect the ventilatory response to hypercapnia when given alone or in combination with meptazinol, but when given with the dextropropoxyphene/paracetamol mixture, a significant reduction in the slope of the ventilatory response to hypercapnia occurred at 1.5 h (P < 0.05) and 2 h (P < 0.01) after administration of the analgesic drug. 4 No pharmacokinetic interaction was demonstrated between ethanol and meptazinol or the dextropropoxyphene/paracetamol mixture in the doses used. 5 In contrast to meptazinol, the dextropropoxyphene/paracetamol mixture interacts with ethanol on the ventilatory function.

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“…Samples were preserved with sodium fluoride and, in the case of blood, heparin. Alcohol levels were estimated by the method of Curry, Walker, and Simpson (1966).…”

Section: Experimental and Methodsmentioning

confidence: 99%

The Precision of Urine to Blood Alcohol Ratios

Muir

Day

1969

Ann Clin Biochem

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Determination of ethanol in blood by gas chromatography

Cited by 140 publications

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In Vivo α₁-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects

In Vivo α₁-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects

Comparison of the effects of therapeutic doses of meptazinol and a dextropropoxyphene/paracetamol mixture alone and in combination with ethanol on ventilatory function and saccadic eye movements.

The Precision of Urine to Blood Alcohol Ratios

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Determination of ethanol in blood by gas chromatography

Cited by 140 publications

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In Vivo α1-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects

In Vivo α1-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects

Comparison of the effects of therapeutic doses of meptazinol and a dextropropoxyphene/paracetamol mixture alone and in combination with ethanol on ventilatory function and saccadic eye movements.

The Precision of Urine to Blood Alcohol Ratios

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In Vivo α₁-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects

In Vivo α₁-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects