Determination of eugenol in plants and pharmaceutical form by HPLC with amperometric detection at graphene-modified carbon paste electrode

Aydoğmuş, Zeynep; Yildiz, Gulcemal; Yılmaz, Ece Merve; Aboul‐Enein, Hassan Y.

doi:10.1007/s41127-017-0015-4

Cited by 8 publications

(3 citation statements)

References 41 publications

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“…A UV spectrometry technique using liquid–liquid microextraction was applied for the analysis of EOH in personal care products [ 19 ]. Different HPLC methods were also used for the determination of EOH in different plant extracts and herbal preparations [ 19 , 20 , 21 , 22 ]. Various HPTLC techniques have also been reported for the determination of EOH in plant extracts and herbal preparations [ 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Cinnamaldehyde and Eugenol in Essential Oils and Traditional and Ultrasound-Assisted Extracts of Different Species of Cinnamon Using a Sustainable/Green HPTLC Technique

et al. 2021

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A wide range of analytical techniques are reported for the determination of cinnamaldehyde (CCHO) and eugenol (EOH) in plant extracts and herbal formulations either alone or in combination. Nevertheless, sustainable/green analytical techniques for the estimation of CCHO and EOH either alone or in combination are scarce in the literature. Accordingly, the present research was carried out to establish a rapid, highly sensitive, and sustainable high-performance thin-layer chromatography (HPTLC) technique for the simultaneous estimation of CCHO and EOH in the traditional and ultrasound-assisted methanolic extracts of Cinnamomum zeylanicum,C. burmannii, and C. cassia and their essential oils. The simultaneous estimation of CCHO and EOH was performed through NP-18 silica gel 60 F254S HPTLC plates. The cyclohexane/ethyl acetate (90:10, v v−1) solvent system was optimized as the mobile phase for the simultaneous estimation of CCHO and EOH. The greenness score of the HPTLC technique was predicted using AGREE software. The entire analysis was carried out at a detection wavelength of 296 nm for CCHO and EOH. The sustainable HPTLC technique was observed as linear in the range 10–2000 ng band−1 for CCHO and EOH. The proposed technique was found to be highly sensitive, rapid, accurate, precise, and robust for the simultaneous estimation of CCHO and EOH. The content of CCHO in traditional methanolic extracts of C. zeylanicum,C. burmannii, and C. cassia was found to be 96.36, 118.49, and 114.18 mg g−1, respectively. However, the content of CCHO in ultrasound-assisted methanolic extracts of C. zeylanicum,C. burmannii, and C. cassia was found to be 111.57, 134.39, and 129.07 mg g−1, respectively. The content of CCHO in essential oils of C. zeylanicum,C. burmannii, and C. cassia was found to be 191.20, 214.24, and 202.09 mg g−1, respectively. The content of EOH in traditional methanolic extracts of C. zeylanicum,C. burmannii, and C. cassia was found to be 73.38, 165.41, and 109.10 mg g−1, respectively. However, the content of EOH in ultrasound-assisted methanolic extracts of C. zeylanicum,C. burmannii, and C. cassia was found to be 87.20, 218.09, and 121.85 mg g−1, respectively. The content of EOH in essential oils of C. zeylanicum,C. burmannii, and C. cassia was found to be 61.26, 79.21, and 69.02 mg g−1, respectively. The amounts of CCHO and EOH were found to be significantly higher in ultrasound-assisted extracts of all species compared to its traditional extraction and hence ultrasound extraction has been proposed as a superior technique for the extraction of CCHO and EOH. The AGREE analytical score of the present analytical technique was predicted as 0.75, suggesting excellent greenness profile of the proposed analytical technique. Based on all these observations and results, the proposed sustainable HPTLC technique can be successfully used for the simultaneous estimation of CCHO and EOH in different plant extracts and herbal products.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Cinnamaldehyde and Eugenol in Essential Oils and Traditional and Ultrasound-Assisted Extracts of Different Species of Cinnamon Using a Sustainable/Green HPTLC Technique

et al. 2021

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“…[15][16][17][18][19][20][21][22][23][24][25][26] Various authors have developed and reported different HPTLC, HPLC, and UV method, for the estimation of EGL alone, as well as combinations with cinnamon oil, rosmarinic acid, piperine, and cinnamaldehyde. [27][28][29][30][31][32][33][34][35][36][37][38] Attempt was made to develop and validate simpler, sensitive, precise, accurate, and cost-effective UV spectroscopic methods for the simultaneous determination of MZL and EGL in emulgel formulation [Figure 2].…”

Section: Introductionmentioning

confidence: 99%

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2023

AJP

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Introduction: Fungal infection remains a continuous and growing threat to human health. Combination therapy may be an optional approach for the treatment of invasive fungal infections, and the potential antifungal mechanisms provide new insights into novel antifungal drug development. Several papers have proven that blend of miconazole nitrate and eugenol has synergistic antifungal effect. Therefore, a formulation used eugenol to increase the skin penetration and increase the availability of miconazole nitrate in topical gels. As a result, a method for simultaneous measurement of the aforementioned medicines in the formulation of emulgel has been devised and validated using UV spectrophotometric analysis. Materials and Methods: In the simultaneous equation method, absorbance was measured at 272 and 280 nm for the estimation of both drugs. Miconazole nitrate and eugenol were estimated at 281 and 271 nm in the first derivative (zero crossing) method, respectively. The ratio derivative method utilized peak amplitudes at 283 and 274 nm for miconazole nitrate and 286 and 292 nm for eugenol. The developed methods were validated according to the ICH guidelines, including parameters such as specificity, linearity, range, precision, accuracy, limit of detection, and limit of quantification. Results and Discussion: All three assay methods showed a direct relationship between response and concentration in the concentration range of 100-600 µg/mL for miconazole nitrate and 53-318 µg/mL for eugenol. The level of dispersion was within 2% of the RSD. Recovery studies for both drugs ranged from 97 to 102%, recommending that the methods are effective. Conclusion: All of the proposed techniques were found to be quick, accurate, and inexpensive. As a result, they can now be helpful for routine quality control analysis when calculating miconazole nitrate and eugenol in emulgel formulation or formulations containing the aforementioned medications.

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“…Eugenol has also been determined using liquid chromatography [16] provided with electrochemical [17][18] or UV detection [19], voltammetric [20], photodiode array (PDA) [21] and oscillating methods [22]. These methods are accurate and efficient, but they have some drawbacks such as complexity, expense, and timeconsuming.…”

Section: ■ Introductionmentioning

confidence: 99%

Determination of Eugenol in Personal-Care Products by Dispersive Liquid-Liquid Microextraction Followed by Spectrophotometry Using <i>p</i>-Amino-<i>N,N</i>-dimethylaniline as a Derivatizing Agent

et al. 2020

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Two simple methods for the determination of eugenol were developed. The first depends on the oxidative coupling of eugenol with p-amino-N,N-dimethylaniline (PADA) in the presence of K3[Fe(CN)6]. A linear regression calibration plot for eugenol was constructed at 600 nm, within a concentration range of 0.25-2.50 μg.mL–1 and a correlation coefficient (r) value of 0.9988. The limits of detection (LOD) and quantitation (LOQ) were 0.086 and 0.284 μg.mL–1, respectively. The second method is based on the dispersive liquid-liquid microextraction of the derivatized oxidative coupling product of eugenol with PADA. Under the optimized extraction procedure, the extracted colored product was determined spectrophotometrically at 618 nm. A linear plot within a concentration range of 0.05–1.65 μg.mL–1 (r = 0.9997) was constructed. The LOD and LOQ were 0.053 and 0.177 μg.mL–1, respectively. Both methods were tested for the analysis of eugenol in commercial personal-care products, and the results confirmed that the procedures are accurate, precise, and reproducible (RSD < 1%).

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Determination of eugenol in plants and pharmaceutical form by HPLC with amperometric detection at graphene-modified carbon paste electrode

Cited by 8 publications

References 41 publications

Simultaneous Estimation of Cinnamaldehyde and Eugenol in Essential Oils and Traditional and Ultrasound-Assisted Extracts of Different Species of Cinnamon Using a Sustainable/Green HPTLC Technique

Simultaneous Estimation of Cinnamaldehyde and Eugenol in Essential Oils and Traditional and Ultrasound-Assisted Extracts of Different Species of Cinnamon Using a Sustainable/Green HPTLC Technique

Untitled

Determination of Eugenol in Personal-Care Products by Dispersive Liquid-Liquid Microextraction Followed by Spectrophotometry Using <i>p</i>-Amino-<i>N,N</i>-dimethylaniline as a Derivatizing Agent

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