Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry

Jing, Nianhua; Shi, Junyou; Li, Guoliang; Sun, Zhi‐Wei; You, Jing

doi:10.1016/j.foodres.2012.02.014

Cited by 13 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection limits (S/N = 3, signal-tonoise ratio of 3:1) of the EPA, DHA, and AA were 0.13, 0.14, and 0.23 μM, respectively. The quantitative sensitivity was compared with other available methods for determining EPA, 24 were 51.55 μM, 1.63 μM, 0.33 μM, 0.1 μM, and 4.73 nM, respectively. The present HPLC-ECD for determining EPA, DHA, and AA was highly sensitive in comparison with GC-MS 22 and HPLC-UV, 23 and the present HPLC-ECD provided similar sensitivity to LC-MS/MS.…”

Section: Hplc-ecd For Determining Epa Dha and Aamentioning

confidence: 99%

“…26 However, the present HPLC-ECD was less sensitive in comparison with the LC/MS based on DBD-PZ-NH2 derivatization 25 and the HPLC-FL based on BAETS derivatization. 24 In an analysis of biological samples by LC/MS and HPLC-FL, the procedure of derivatization and cleanup of the samples is sometimes complicated and time-consuming. Thus, the present HPLC-ECD, which does not require a derivatization procedure to detect EPA, DHA, and AA, would be a favorable method in an analysis when using biological samples.…”

Section: Hplc-ecd For Determining Epa Dha and Aamentioning

confidence: 99%

“…19,20 To help the effective progression of these clinical studies, the use of a simple, selective, and sensitive analytical method for determining EPA, DHA, and AA should be of significant importance. Gas chromatography with flame ionization detection (GC-FID), 21 GC with mass spectrometry (GC-MS), 22 high-performance liquid chromatography with UV detection (HPLC-UV), 23 HPLC with fluorescent detection (HPLC-FL), 24 LC with mass spectrometry (LC-MS), 25 and LC-tandem mass spectrometry (LC-MS/MS) 26,27 have been applied to determine EPA, DHA, and AA. However, in GC-MS, HPLC-UV, HPLC-FL, and LC-MS, the cleanup and derivatization procedures are essential for the sensitive and selective determination of EPA, DHA and AA in real sample analysis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Eicosapentaenoic, Docosahexaenoic, and Arachidonic Acids in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection

et al. 2016

View full text Add to dashboard Cite

A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) system was developed for the simultaneous determination of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and arachidonic acid (AA) in human plasma. In the present HPLC-ECD system, EPA, DHA, and AA were separated using a reverse-phase C30 column and detected based on the voltammetric reduction of 3,5-di-tert-butyl-1,2-benzoquinone (DBBQ). Chromatographic peak areas were proportional to the concentration of EPA, DHA, and AA from 0.75 μM to 0.1 mM (r > 0.998). The concentrations of EPA, DHA, and AA in plasma from healthy human subjects after overnight fasting were determined, and the ratio of EPA to AA was obtained by the present HPLC-ECD method, which required 40 μL of human plasma and a simple procedure of sample preparation using diethyl ether extraction. Moreover, changes in EPA, DHA, and AA concentrations in a human subject were monitored before and after fish oil supplement administration by the present HPLC-ECD system.

show abstract

Section: Hplc-ecd For Determining Epa Dha and Aamentioning

confidence: 99%

Section: Hplc-ecd For Determining Epa Dha and Aamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Eicosapentaenoic, Docosahexaenoic, and Arachidonic Acids in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection

et al. 2016

View full text Add to dashboard Cite

show abstract

“…FAs are nonvolatile and exhibit neither natural UV absorption nor fluorescence property. Therefore, GC or liquid chromatography (LC) method in combination with derivatization were the most often used methods for the analysis of them [18][19][20][21][22][23]. GC and GC-MS methods have been traditionally applied to the analysis of fatty acids since 1950s [24].…”

Section: Introductionmentioning

confidence: 99%

“…2‐(9‐Oxoacridin‐10(9H)‐yl) acetohydrazide (OAAH) with excellent fluorescence properties was chosen as derivatization reagent because it is easy to synthesize. Besides, the derivatization of OAAH with FAs could be carried out under mild conditions within 20 min, much less than the often used 30 min of other derivatization methods . The proposed method was successfully applied to the analysis of total fatty acids (TFAs) and free fatty acids (FFAs) in four kinds of Dendrobium species.…”

Section: Introductionmentioning

confidence: 99%

A sensitive high‐performance liquid chromatography method with fluorescence detection for the determination of fatty acids as exemplified for Dendrobium species

Zhang

Song

Chen

et al. 2013

Euro J Lipid Sci & Tech

Self Cite

View full text Add to dashboard Cite

A novel high‐performance liquid chromatography (HPLC) method using 2‐(9‐oxoacridin‐10(9H)‐yl) acetohydrazide (OAAH) as pre‐column labeling reagent was applied to the analysis of fatty acids in four kinds of Dendrobium species for the first time. The reaction of OAAH with fatty acids could proceed easily and quickly in the presence of 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide hydrochloride (EDC) as condensing reagent within 20 min. The derivatives exhibit excellent fluorescence property with excitation and emission wavelengths of 255 and 420 nm, respectively. The 20 fatty acid derivatives were separated on a SB C18 reversed‐phase column with gradient elution. Good linear correlations were observed for all fatty acids with correlation coefficients of >0.996. When 50 mg of sample was used for analysis, the detection limits at a signal‐to‐noise ratio of 3 were in the range of 0.10–0.42 µg/g. Free fatty acids and total fatty acids in four kinds of Dendrobium species were analyzed by the developed method. The results indicated that the main fatty acids in Dendrobium species were unsaturated linoleic acid (C18:2n‐6) and saturated hexadecanoic acid. This work provides a useful tool for the safety assessment and quality control of Dendrobium species. Practical applications: Dendrobium species, the stems of which can be used for medical purpose or as health foods, are precious plants in China. Their significant medical effects have recently attracted increasing attention. However, there is little research on the fatty acid composition of Dendrobium species. The proposed method provided a sensitive method for the HPLC analysis of FFAs and TFAs in four kinds of Dendrobium species. This is the first report of the fatty acid compositions of Dendrobium species. It would be helpful for the comprehensive safety or quality assessment of Dendrobium species.

show abstract

Coprinus comatus (O.F. Müll.) Pers. - AGARICACEAE

Gafforov,

Rašeta,

Yarasheva

et al. 2023

Ethnobiology

View full text Add to dashboard Cite

Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry

Cited by 13 publications

References 35 publications

Determination of Eicosapentaenoic, Docosahexaenoic, and Arachidonic Acids in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection

Determination of Eicosapentaenoic, Docosahexaenoic, and Arachidonic Acids in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection

A sensitive high‐performance liquid chromatography method with fluorescence detection for the determination of fatty acids as exemplified for Dendrobium species

Coprinus comatus (O.F. Müll.) Pers. - AGARICACEAE

Contact Info

Product

Resources

About