Determination of Fenthion Residues in Plant and Animal Tissues by Electron-Capture Gas Chromatography

Abstract

tographic cleanup methods. The authors' years of experience with the apparatus (8) indicate that up to six to eight samples of 0.5 ml. each of concentrated citrus extractives can be cleaned up before the stripping tube is overloaded. From the injection of the sample to the final recovery of the solvent-free concentrated extractive, which is directly usable for gas chromatographic analysis, not more than 30 minutes of elapsed time are required. Since it is hoped that this procedure will also be found advantageous for the cleanup of volatile pesticide-containing extracts prior to paper or thin-layer and gas. chromatographic analysis, construction details of the apparatus are given.

Determination of fenthion and five of its metabolites in corn, grass, and milk

“…A method was needed to determine residues of this chemical in corn and grass intended for use as feed for livestock and in the milk of cows fed forage containing residues of the insecticide. The need to determine metabolites of the parent compound is apparent from the work of several investigators who reported on metabolites formed from fenthion in plants and animals (Anderson et al, 1966;Brady and Arthur, 1961;Niessen et al, 1962).…”

“…In a method of determining fenthion residues recently reported (Anderson et al, 1966), fenthion and the five metabolites are oxidized to the O-analog sulfone (VI) which is then hydrolyzed to the corresponding phenol. The phenol is brominated and acetylated and then analyzed by electron-capture gas chromatography.…”

Pesticide residues

“…Anderson and coworkers (7) determined Fenthion and its five metabolites in plant and animal tissues by electron capture GLC. The samples are extracted with hexane or acetone.…”

Method to determine fenthion and five oxidative metabolites in fat

“…Since the lipophilic properties of fenthion are well known (Mollhoff, 1971), one would expect greater residues of these compounds in fat than in muscle tissue of treated cattle. Methods for the extraction, separation, and quantitation of fenthion and metabolites from a variety of biological samples are available in Anderson et al (1966), Beroza (1968,1969), and Suffet et al (1967). Bowman and Beroza (1968) were the only ones to develop a method by which all six compounds could be analyzed individually by gas chromatography.…”