Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

Cid, Yara Peluso; Ferreira, Thaís Paes; Medeiros, Deborah M.V.; Oliveira, Rodrigo M.; Silva, Nathália Cristina Cirone; Magalhães, Viviane de Souza; Scott, Fábio Barbour

doi:10.1590/s0100-40422012001000029

Cited by 9 publications

(8 citation statements)

References 16 publications

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“…Previous results reported that after a repeated oral administration, fipronil concentrations in rat plasma were lower than the LOQ (<100ng/mL) while fipronil sulfone concentrations ranged from 1 to 2 µg/mL (Lacroix et al 2010). The LOQ of fipronil sulfone was 5 ng/mL (Cid et al 2012) and it ranged from 0.05-0.16 and 0.28-0.73 µg/mL for fipronil and fipronil sulfone, respectively (Bichon et al 2008). Blank (control group of rats) samples were spiked with 3 levels (1, 5, 10 µg/g) of each compound and were read in triplicates.…”

Section: Methods Limit Of Quantification (Loq) Precision and Accuracymentioning

confidence: 99%

“…Also, HPLC method with UV detection (Leghait et al 2009) was used for fipronil determination in rats' plasma and showed that fipronil was actively metabolized to its sulfone derivative. The reversed-phase LC-UV (at 210 nm) was efficient in the determination of fipronil in bovine plasma after SPE extraction (SPE) (Cid et al 2012), plasma samples using GC-MS/MS (Bichon et al 2008), LC-UV/MS and GC-MS, LC-MS/MS in animal urine samples (Cravedi et al 2013), in human and rodents urine and serum sample using the liquid chromatography/time-of-flight mass spectrometer (LC/TOF-MS) (McMahen et al 2015), and in blood samples of rat and human using LC-MS/MS (Raju et al 2016). Fipronil and its residues were extracted using solid-phase microextraction (SPME; fused-silica fiber coated with 85 mm polyacrylate) (Vıĺchez et al 2001) Because of the limited studies that investigate the movement and transformation of fipronil in various internal tissues of mammals, the current study aimed to detect and determine fipronil and fipronil sulfone in tissues of internal organs of white albino rats using gas chromatography coupled with mass spectroscopy (GC-MS) after oral administration of rats of repetitive sublethal doses of fipronil for 14 days.…”

Section: Introductionmentioning

confidence: 99%

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Determination of fipronil insecticide and its sulfone metabolite in internal organs of white albino rats

Salim

2020

IJPT

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Fipronil is a popular insecticide against a wide range of household, urban, agricultural, animal pests. It gets converted into several metabolites in animals and humans but mainly to fipronil sulfone, which is more toxic and persistent. Therefore, the current study aimed to investigate the presence of fipronil and fipronil sulfone in tissues of internal organs of white albino rats using gas chromatography coupled with mass spectroscopy (GC-MS) after oral administration of rats of repetitive sublethal doses of fipronil for 14 days. Results showed that the R2 values of standard curves of detected compounds were 0.9871 and 0.9989 for FIP and fipronil sulfone, respectively with LOQ of fipronil and fipronil sulfone of 5 and 10 µg/g. Average recovery percentages were from 96.3±5.4 to 101±8.5% and 93.8±4.7 to 98.4±5.1% for fipronil and fipronil sulfone, respectively with RSD values lower than 10%. Fipronil was detected in all tested organs. It was concentrated with great amounts in the lungs, liver, and kidney followed by testes, spleen, and brain, while fipronil sulfone was detected in the liver, testes, spleen, and lungs tissues in descending order. Fipronil was biotransformed into fipronil sulfone at different organs of white albino rats and the GC-MS coupled with the QuEChERS extraction and clean-up method were efficient in detecting them in the internal organs of rats.

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Section: Methods Limit Of Quantification (Loq) Precision and Accuracymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of fipronil insecticide and its sulfone metabolite in internal organs of white albino rats

Salim

2020

IJPT

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“…The mobile phase consisted of acetonitrile: water (60:40, v/v) with a flow rate of 1.0 mL/min. The UV wavelength was set at 210 nm and the injection volume was 10 μL (22).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Fipronil Tablets: Development and Pharmacokinetic Profile in Beagle Dogs

et al. 2019

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Increased human-pet interactions have led to concerns related to the prevention and treatment of ectoparasite infestations. Fipronil (FIP) is a widely used ectoparasiticide in veterinary medicine available for topical administration; however, its use may cause damage to the owners and the environment. The aim of the study was to develop immediate-release tablets of FIP, as well as to determine its pharmacokinetic properties after oral administration in beagle dogs. The prepared FIP tablets were evaluated for precompression (angle of repose, speed flow, and Carr's index) and post-compression (weight variation, friability, thickness, hardness, disintegration time, and dissolution rate) parameters. Orally administered FIP at a dose of 2 mg/kg was rapidly absorbed with C máx of 3.13 ± 1.39 μg/mL at 1.83 ± 0.40 h post treatment (P.T.) and metabolized with 1.27 ± 1.04 μg/mL at 2.33 ± 0.82 h P.T. for fipronil sulfone (SULF) (the primary metabolite). The elimination of FIP and SULF occurred slowly and had maintained quantifiable plasma levels in the blood for up to 28 days P.T. The goal of the study is aligned with the concept of One Health, which aims to collaboratively achieve the best health for people, animals, and the environment. Therefore, the use of FIP tablets for the control of ectoparasites in dogs may be a safer alternative for owners and the environment.

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“…It is relatively stable in water, but it´s not so stable in soil, due to its photolysis. Thus, the development of a precise, exact, rapid and efficient method of its quantification is really actual task [14][15][16].…”

Section: Fipronilmentioning

confidence: 99%

Kobalt (III) Oksihidroksit Yardımıyla Fipronilin Elektrokimyasal Tespiti İçin Matematiksel Açıklama

Tkach¹,

Ivanushko²,

Lukanova³

et al. 2017

Akademik Gıda

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The possibility of fipronil pesticide electrochemical determination assisted by cobalt (III) oxyhydroxide was determined in this study. The redox pair CoO(OH)/CoO2 was used in this process. The correspondent mathematical model was developed and analyzed by means of linear stability theory and bifurcation analysis. It was shown that the oxyhydroxide may be applied as an efficient electrode modifier in the detection of fipronil in neutral and lightly alkaline media. Electrochemical response was clear and easy to interpret. The possibility of oscillatory and monotonic instabilities was also verified.

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Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

Cited by 9 publications

References 16 publications

Determination of fipronil insecticide and its sulfone metabolite in internal organs of white albino rats

Determination of fipronil insecticide and its sulfone metabolite in internal organs of white albino rats

Fipronil Tablets: Development and Pharmacokinetic Profile in Beagle Dogs

Kobalt (III) Oksihidroksit Yardımıyla Fipronilin Elektrokimyasal Tespiti İçin Matematiksel Açıklama

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