Determination of Flavonoid Glycosides by UPLC-MS to Authenticate Commercial Lemonade

Xue, Ying; Qing, Lin-Sen; Li, Yong; Xu, Xian-Shun; Hu, Bin; Tang, Ming-Qing; Xie, Jing

doi:10.3390/molecules24163016

Cited by 11 publications

(10 citation statements)

References 31 publications

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“…The determination of diosmin along with other bioflavonoids is commonly performed by chromatographic [ 10 , 11 , 12 ], spectrophotometric [ 13 , 14 , 15 ] or fluorimetric [ 16 ] methods. Electroanalytical techniques are less exploited, although they have some economic advantages and convenient characteristics such as simplicity, speed and small amount of waste.…”

Section: Introductionmentioning

confidence: 99%

Disposable Pencil Graphite Electrode for Diosmin Voltammetric Analysis

et al. 2021

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Diosmin (DIO) is a naturally occurring flavonoid with multiple beneficial effects on human health. The presence of different hydroxyl groups in diosmin structure enables its electrochemical investigation and quantification. This work presents, for the first time, diosmin voltammetric behavior and quantification on the cost-effective, disposable pencil graphite electrode (PGE). Diosmin oxidation on PGE involves two irreversible steps, generating products with reversible redox behaviors. All electrode processes are pH-dependent and predominantly adsorption-controlled. Differential pulse (DPV) and adsorptive stripping differential pulse (AdSDPV) voltammetric methods have been optimized for diosmin quantification o an H-type PGE, in 0.100 mol/L H2SO4. The linear ranges and limits of detection were for DPV 1.00 × 10−6–1.00 × 10−5 mol/L and 2.76 × 10−7 mol/L DIO for DPV and 1.00 × 10−7–2.50 × 10−6 mol/L and 7.42 × 10−8 mol/L DIO for AdSDPV, respectively. The DPV method was successfully applied for diosmin quantification in dietary supplement tablets. The percentage recovery was 99.87 ± 4.88%.

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Section: Introductionmentioning

confidence: 99%

Disposable Pencil Graphite Electrode for Diosmin Voltammetric Analysis

et al. 2021

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“…Negative ion mode of MS E /Fast DDA has showed much more abundance daughter ions than those of the positive ion mode, and thus it was used for the structure characterization. After comparing the parent‐to‐daughter ions with in‐house database in UNIFI platform and the references [22–28], 14 flavonoids were observed and identified. Nine compounds, including rutin (Cpd 15), LQ (Cpd 16), ILQ (Cpd 25), LG (Cpd 27), quercetin (Cpd 29), ILG (Cpd 38), FM (Cpd 41), LCC (Cpd 52), and LFB (Cpd 56) were confirmed by comparing their accurate parent and daughter ions of Fast DDA spectra and retention times with those of reference compounds.…”

Section: Resultsmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 99%

Chemical profiling of Honghua Xiaoyao tablet and simultaneous determination of its quality markers by liquid chromatography–tandem mass spectrometry combined with chemometrics methods

Zhang

et al. 2020

J of Separation Science

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Discovering marker components of traditional Chinese medicine formulas is challenging because of the hundreds of components they inherently contain. This study first proposed a reliable and validated method for the comprehensive profiling of chemical constituents in Honghua Xiaoyao tablet by using high-performance liquid chromatography coupled with mass spectrometry. After searching within the in-house library, a total of 55 constituents were unambiguously characterized or tentatively identified through reference standards and by comparing mass spectrometry data with literature values. Quantitative analysis of 14 compounds, which were selected as the quality marker components based on a serum pharmacochemistry study, has been performed by triple-quardrupole mass spectrometry technique. Multiple chemometric methods, including principal components analysis and hierarchical cluster analysis, were subsequently used to analyze the quantitative results, classify samples from three manufacturers, and distinguish the analytical markers. In method validation results, 14 quality marker compounds have shown good linearity (R 2 ≥ 0.9965) with a relative wide concentration range and acceptable recovery at 98.39-102.46%. The proposed approach provides the chemical evidence for revealing the material basis of Honghua Xiaoyao tablet, and establishes a reliable statistical analysis-based strategy of quality marker investigation for controlling its quality.

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“…The results showed that the regression equation of eriocitrin was y ¼ 523.81x À 82.61 (r ¼ 0.996), the linear range was 2.01-50.3 ng mL À1 , the intra-day precision (RSD) was 1.36%, while the inter-day precision (RSD) was 3.53% and recovery rates were 88.5%. 5 In addition, a reversed phase expressed as i pa (mA) ¼ 60.91C (mM) + 0.8447 (r ¼ 0.998) and i pa (mA) ¼ 0.864 + 0.0580c (r ¼ 0.996), respectively. Compared with the above literature, the LC-MS/MS method adopted in this experiment has high sensitivity, good linearity and feasibility, which is suitable for the pharmacokinetic study of eriocitrin.…”

Section: Methods Validationmentioning

confidence: 99%

“…Epidemiological and clinical studies show that diets with a high antioxidant capacity are closely related to signicant decreases in the plasma C-reactive protein, ischemic stroke, or the overall risk of cardiovascular disease or colorectal cancer. 1 Eriocitrin, (eriodictyol 7-O-beta-rutinoside), as the main ingredient in citrus fruits (lemon, citrus, and grapefruit), [2][3][4][5] vegetables, and processed products (drinks, wine), 6 has aroused extensive interests of the scientic community due to its strong antioxidant activity, [7][8][9] which is stronger than that of hesperidin and naringin. Modern pharmacological studies show that eriocitrin has strong lipid-lowering, anticancer and anti-inammatory activities.…”

Section: Introductionmentioning

confidence: 99%