Determination of flumequine and doxycycline in milk by a simple thin-layer chromatographic method

Choma, Irena; Grenda, Dorota; Malinowska, Irena; Suprynowicz, Z.

doi:10.1016/s0378-4347(99)00328-x

Cited by 49 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Currently, methods such as microbiological and thin-layer chromatography have been described for monitoring TCs in biological matrices (Choma, Grenda, Malinowska, & Suprynowicz, 1999;Kurittu, Lonnberg, Virta, & Karp, 2000), however, some of them were either time consuming or precision variable and even cannot identify certain TCs. Therefore, a precise chromatographic analysis method for the TCs is needed.…”

Section: Introductionmentioning

confidence: 99%

Development of an HPLC–UV method for the simultaneous determination of tetracyclines in muscle and liver of porcine, chicken and bovine with accelerated solvent extraction

Tao

Chen

et al. 2011

Food Chemistry

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Development of an HPLC–UV method for the simultaneous determination of tetracyclines in muscle and liver of porcine, chicken and bovine with accelerated solvent extraction

Tao

Chen

et al. 2011

Food Chemistry

View full text Add to dashboard Cite

“…Several analytical procedures are currently available for the determination of TC residues in animal tissues and at present chromatographic techniques are mainly used as confirmatory ones for these substances. Despite the simplicity and the low cost of TLC [14,15], HPLC coupled with UV [9, 16 -18], fluorescence [13,19], and MS [20 -23] remains the technique of choice for multiresidue analyses. Isolation of TCs from tissues includes an extraction step with a suitable solvent system and a clean-up procedure.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Samanidou

Nikolaidou

Papadoyannis³

2005

J of Separation Science

View full text Add to dashboard Cite

A high-performance liquid chromatographic method with diode-array detection, at 351 nm, was developed and validated for the determination of five tetracyclines (TCs): minocycline, tetracycline, oxytetracycline, chlortetracycline, and doxycycline in bovine muscle. Samples were macerated with a buffer solution, centrifuged, and purified using Abselut Nexus SPE cartridges. The separation of the examined TCs was achieved on an Inertsil ODS-3 5 microm, 250 x 4 mm analytical column, at ambient temperature. A multistep gradient elution was followed using 0.05 M oxalic acid and CH3CN, at a flow rate of 1.65 mL/min. The procedure was validated according to the European Union regulation 2002/657/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. The results of the validation process demonstrate that the method can be readily applied to European Union statutory veterinary drug residue surveillance programmes. Mean recoveries of TCs from bovine muscle samples spiked at three concentrations (100, 250, and 400 ng/g) were in the range of 98.7-103.3%. Method's LOQ values achieved were 40 microg/kg for MNC, CTC, and DC and 25 microg/kg for OTC and TC. The decision limits (CCalpha) were in the range of 104.7-109.8 microg/kg, while the detection capability (CCbeta) was in the range of 108.4-116.7 microg/kg for all compounds.

show abstract

“…However, this may lead to occurrence of crooked front of the mobile phase or tailing effect, particularly, when the complex matrix samples are analyzed. In order to obtain a regular front of developing solvent, standard solutions and milk samples were applied in the middle of trapezoidal-shaped regions prepared by incision in the TLC plate's concentrating zone [11,12]. During the development of the chromatogram, large protein molecules have remained on the concentrating zones.…”

Section: Resultsmentioning

confidence: 99%

Stability of cefacetrile in milk samples with various fat content

Grzelak

Choma

2013

Eur Food Res Technol

View full text Add to dashboard Cite

The consumers should have access to food that is safe and free of contaminants. Unfortunately, the wide use of antibiotics in livestock, not only for medicinal purposes but also as illegal feed additives, may be the reason for emergence of the antibiotic residues in foodstuffs of animal origin. Milk is one of the most essential nutrients for humans during their lifetimes. Failure of the withdrawal periods, or what is highly prohibited but in spite of this increasingly practiced, addition of the antibiotics to milk in order to prolong its freshness, may lead to occurrence of their residues. Cefacetrile is a first generation cephalosporin that is commonly use for the treatment of bovine mastitis. The aim of this work was to determine the stability of cefacetrile in milk samples of different fat content, that is, 0 , 2 , 3.2 % UHT, 2 % pasteurized and *3.9 % raw cattle milk, for 5 days. Two chromatographic methods were applied and compared for this purpose, that is, thin-layer chromatography and highperformance liquid chromatography. According to the results coming from HPLC-UV analysis, the stability of cefacetrile increase with the increase in the fat mass content in UHT milk. The cefacetrile level in pasteurized and raw cattle milk is similar to that in 3.2 % UHT milk. For the TLC method, this relation can be observed only in the second day of the analysis. The spiked milk samples analyzed by HPLC were deproteinized with MeCN and stored for 5 days of the experiment. Whereas, in the TLC-UV method, the spiked milk samples prepared in the first day of experiment were analyzed directly, without deproteinization. The lower stability of cefacetrile in milk samples observed from the TLC analysis can be related to its partial enzymatic decomposition.

show abstract

Determination of flumequine and doxycycline in milk by a simple thin-layer chromatographic method

Cited by 49 publications

References 17 publications

Development of an HPLC–UV method for the simultaneous determination of tetracyclines in muscle and liver of porcine, chicken and bovine with accelerated solvent extraction

Development of an HPLC–UV method for the simultaneous determination of tetracyclines in muscle and liver of porcine, chicken and bovine with accelerated solvent extraction

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Stability of cefacetrile in milk samples with various fat content

Contact Info

Product

Resources

About