Determination of fluoroquinolones in chicken tissues by LC‐coupled electrospray ionisation and atmospheric pressure chemical ionisation

Bailac, S; Barrón, D.; Sanz‐Nebot, Victoria; Barbosa, Johildo Salomão Figueirêdo

doi:10.1002/jssc.200500219

Cited by 32 publications

(18 citation statements)

References 32 publications

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“…Electrospray mass spectra (ESI-MS) of seven floroquionolones other than norfloxacin have already been characterized in chicken tissue samples (Bailac et al, 2005). In this study norfloxacin and ciprofloxacin in plasma samples were studied by means of APCI-MS technique.…”

Section: Ms Spectra Interpretationmentioning

confidence: 99%

“…Recently, a liquid-chromatography method with fluorescence detection was developed, which can be applied to the reciprocal determination of furosemide or norfloxacin in plasma samples, using each of them as internal standard for the determination of the other (Galaon et al, 2007). LC-coupled electrospray and atmospheric pressure chemical ionization technique has been used for the determination of seven quinolones in chicken tissues using norfloxacin as internal standard (Bailac et al, 2006). Recently, an LC-MS/ MS method for the determination of ulifloxacin, the active metabolite of prulifloxacin, in human plasma has been reported (Guo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a new norfloxacin metabolite monitored during a bioequivalence study by means of mass spectrometry and quantum computation

Medvedovici

Sora²,

Ionescu

et al. 2008

Biomedical Chromatography

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A bioequivalence study of two formulations containing norfloxacin was used for identification and assay of the metabolite of norfloxacin in human serum samples. The bioequivalence study was based on an analytical method using liquid chromatography with fluorescence detection. The plasmatic profile of metabolite was similar to norfloxacin for both formulations. Three plasma fractions of norfloxacin metabolite from a volunteer were isolated by liquid chromatography and investigated by atmospheric-pressure chemical ionization mass-spectrometry. The structure of norfloxacin metabolite (7-aminoethylenamino-6-fluoro-4-hydroxy quinoline-3-carboxylic acid) was identified taking into account also the mass spectrometry investigations achieved for norfloxacin and ciprofloxacin (used as internal standard for the analytical method). A theoretical procedure based on quantum chemical calculations has been also used to explain the mass fragmentation in molecules of norfloxacin metabolite that differ from the molecule of norfloxacin.

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Section: Ms Spectra Interpretationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of a new norfloxacin metabolite monitored during a bioequivalence study by means of mass spectrometry and quantum computation

Medvedovici

Sora²,

Ionescu

et al. 2008

Biomedical Chromatography

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“…Most of them involve liquid chromatography with ultraviolet (LC-UV; Gigosos et al, 2000;Pecorelli et al, 2003;Bailac et al, 2004;Christodoulou and Samanidou, 2007), fluorescence (LC-FD;Eng et al, 1998;Hernández-Arteseros et al, 2000;Chu et al, 2002;EspinosaMansilla et al, 2005;Hassouan et al, 2007) or mass spectrometric detection (Van Vyncht et al, 2002;Toussaint et al, 2002Toussaint et al, , 2005aSchneider et al, 2005;Van Hoof et al, 2005;Hermo et al, 2006;Bailac et al, 2006;Rubies et al, 2007).…”

Section: Introductionmentioning

confidence: 98%

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

Zafra-Gómez

Garballo

Ballesteros

et al. 2008

Biomedical Chromatography

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A new liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for the simultaneous determination of eight quinolone antibacterials for veterinary use in processed bovine milk samples. The quinolones studied included marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid and flumequine. Also, a new sample-treatment procedure was used for extraction and preconcentration of these compounds. It involved defatting by centrifugation, protein precipitation by adding a mixture of glacial acetic acid-acetonitrile and removing acetonitrile with dichloromethane; finally, the acidified aqueous layer was evaporated to dryness in a speed vac system, resuspended in the mobile phase and filtered prior to LC injection. The mobile phase was composed of a formic acid aqueous solution 0.1% (v/v) and acetonitrile, with an initial composition of water-acetonitrile 95: 5 (v/v) and using linear gradient elution. Norfloxacin was used as internal standard. The limits of quantification found (2-7 ng g(-1)) were in all cases lower than the maximum residue limits tolerated by the European Union for these compounds in milk.

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“…Two more articles, by Bailac et al [8,9], discuss a method for the determination of seven quinolones. Delepine et al [10] and Choma [11] have proposed methods for the determination of six fluoroquinolones.…”

Section: Introductionmentioning

confidence: 98%

Development and validation of an HPLC confirmatory method for residue analysis of ten quinolones in tissues of various food‐producing animals, according to the European Union Decision 2002/657/EC

Christodoulou

Samanidou²,

Papadoyannis

2007

J of Separation Science

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The aim of this work was to develop an HPLC method for the simultaneous determination of ten quinolones: enoxacin, ofloxacin, norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, oxolinic acid, nalidixic acid, and flumequine, in various tissues of food-producing animals. Separation was achieved on a PerfectSil Target column (250 mm x 4 mm, ODS-3, 5 microm), by MZ-Analysentechnik (Germany), at room temperature. The mobile phase consisted of 0.1% TFA-CH(3)OH-CH(3)CN and was delivered by a gradient program of 35 min. The detection and quantitation was performed on a photodiode array detector at 275 and 255 nm. Caffeine (7.5 ng/microL) was used as the internal standard (IS). Analytes were isolated from tissue samples by 0.1% methanolic TFA solution. SPE, using LiChrolut RP-18 cartridges, was applied for further purification. The extraction protocol was optimized and the final recoveries varied between 92.0 and 107.4%. The method was fully validated according to Commission Decision 2002/657/EC. Limits of quantitation for the examined quinolones extracted from each tissue were much lower than the respective Maximum Residue Levels, ranging between 30 and 50 microg/kg for bovine tissue, between 30 and 55 microg/kg for ovine tissue, and between 40 and 50 microg/kg for porcine tissue.

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Determination of fluoroquinolones in chicken tissues by LC‐coupled electrospray ionisation and atmospheric pressure chemical ionisation

Cited by 32 publications

References 32 publications

Characterization of a new norfloxacin metabolite monitored during a bioequivalence study by means of mass spectrometry and quantum computation

Characterization of a new norfloxacin metabolite monitored during a bioequivalence study by means of mass spectrometry and quantum computation

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

Development and validation of an HPLC confirmatory method for residue analysis of ten quinolones in tissues of various food‐producing animals, according to the European Union Decision 2002/657/EC

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