Determination of Folate Derivatives in Rat Tissues during Folate Deficiency.

Nakata, Rieko

doi:10.3177/jnsv.46.215

Cited by 8 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a folate deficiency study with rats [8], a significant difference was determined even after 3 weeks. In the latter study, hematocrit decreased dramatically from 40 to 20% after 4 to 6 weeks.…”

Section: Discussionmentioning

confidence: 99%

“…Folate deficiency studies have been conducted predominantly on rats [8,9,10,11], as their relatively higher tissue weight and the use of whole organs for folate analysis does not necessarily require sensitive methods. Another advantage of rats is the fact that some strains (e.g., F344) [12] are more susceptible to folate/methyl-deficient diets and hypomethylation than the nowadays most commonly used mice (e.g., C57BL/6J mice) [13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization and Interrelations of One-Carbon Metabolites in Tissues, Erythrocytes, and Plasma in Mice with Dietary Induced Folate Deficiency

2017

View full text Add to dashboard Cite

Studies on one-carbon metabolism for the assessment of folate deficiency have focused on either metabolites of folate metabolism or methionine cycle. To bridge the gap between deficiency markers in these pathways we designed a dietary induced folate deficiency study using male C57BL/6N mice. After weaning (3 weeks) mice were fed a defined control diet (1 week) before being fed a folate deficient diet (n = 6 mice) and the control diet (n = 6 mice) for 12 additional weeks. Thereafter, we determined total homocysteine in plasma and folate in erythrocytes as well as S-adenosylmethionine, S-adenosylhomocysteine, and six folate vitamers in tissues including 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, 5,10-methenyltetrahydrofolate, tetrahydrofolate, 10-formylfolic acid, and folic acid by means of stable isotope dilution assays coupled with liquid chromatography tandem mass spectrometry. In all organs, except heart (mainly 5-mehtyltetrahydrofolate), tetrahydrofolate constitutes the main vitamer. Moreover, in liver tetrahydrofolate was most abundant followed by 5-methyltetrahydrofolate (heart: tetrahydrofolate), 5-formyltetrahydrofolate, and 5,10-methenyltetrahydrofolate. Because of the significant decrease (p < 0.05) of folate status and S-adenosylmethionine/S-adenosylhomocysteine ratio accompanied with increasing S-adenosylhomocysteine (p < 0.05), hepatocytes are most susceptible to folate deficiency. To the best of our knowledge, we herein present the first method for simultaneous quantitation of eight metabolites for both folate and methionine cycle in one tissue sample, tHcy in plasma, and erythrocyte folate to shed light on physiological interrelations of one-carbon metabolism.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization and Interrelations of One-Carbon Metabolites in Tissues, Erythrocytes, and Plasma in Mice with Dietary Induced Folate Deficiency

2017

View full text Add to dashboard Cite

show abstract

“…Pteridines were quantified by high-performance liquid chromatography (HPLC), using the post-column oxidation method [9]. Folate derivatives were measured by HPLC with amperometric detection [10]. The total plasma homocysteine was measured with a commercially available enzymatic assay kit (Axis-Shield, Dundee, UK).…”

Section: Quantification Of Pteridines Folates Homocysteine and Measmentioning

confidence: 99%

Disturbed biopterin and folate metabolism in the Qdpr‐deficient mouse

Xu¹,

Sudo²,

Sanechika³

et al. 2014

FEBS Letters

Self Cite

View full text Add to dashboard Cite

a b s t r a c tQuinonoid dihydropteridine reductase (QDPR) catalyzes the regeneration of tetrahydrobiopterin (BH4), a cofactor for monoamine synthesis, phenylalanine hydroxylation and nitric oxide production. Here, we produced and analyzed a transgenic Qdpr À/À mouse model. Unexpectedly, the BH4 contents in the Qdpr À/À mice were not decreased and even increased in some tissues, whereas those of the oxidized form dihydrobiopterin (BH2) were significantly increased. We demonstrated that unlike the wild-type mice, dihydrofolate reductase regenerated BH4 from BH2 in the mutants. Furthermore, we revealed wide alterations in folate-associated metabolism in the Qdpr À/À mice, which suggests an interconnection between folate and biopterin metabolism in the transgenic mouse model.

show abstract

“…92 National programs for young women and experimental approaches about supplementation of folic acid have been widely performed. [93][94][95][96] As a result of the dramatic changes in food habits among young women, supplementation of micronutrients to young women in Japan should be considered not only from a gynecologic perspective but also from a perinatological perspective.…”

Section: Supplementationmentioning

confidence: 99%

“…Deficiency of folic acid is significantly related with neural tube defects and other congenital anomalies especially when they take valproic acid or carbamazepine for epilepsy treatment 92 . National programs for young women and experimental approaches about supplementation of folic acid have been widely performed 93–96 . As a result of the dramatic changes in food habits among young women, supplementation of micronutrients to young women in Japan should be considered not only from a gynecologic perspective but also from a perinatological perspective.…”

Section: Introductionmentioning

confidence: 99%

Current problems of food intake in young women in Japan: Their influence on female reproductive function

Fujiwara

Nakata

2004

Reprod Medicine & Biology

Self Cite

View full text Add to dashboard Cite

Accumulating evidence suggests that food customs are associated with quality of life in women of the reproductive age. In Japan, dietary limitation for cosmetic purposes, skipping food intake, intake of processed foods and the shift from Japanese to Westernized style food have increased among young women. These changes in food habits can cause inadequate intake of calories, micronutrients, unsaturated fat, phytestrogens and fiber as well as increasing environmental toxins. Furthermore, these food habits increase risk as a result of intake of food additives, anti-oxidants, processing agents and sweeteners, which have been demonstrated to be harmful to human health. These factors are speculated to not only influence the present lifestyle, but also to induce gynecologic disorders such as dysmenorrhea and irregular menstruation. The adverse effects of these dietary habits on pregnancy outcome and carcinogenesis of breast and ovarian cancers have also been demonstrated. In addition, latent development of organic diseases such as endometriosis, which are accompanied by dysmenorrhea, is a concern under the current nutritional environment in young women. Thus, it is an urgent issue to evaluate the present situation of eating habits in young Japanese women and estimate the influence of these habits on the quality of life including reproductive functions. (Reprod Med Biol 2004; 3: 107-114)

show abstract

Determination of Folate Derivatives in Rat Tissues during Folate Deficiency.

Cited by 8 publications

References 23 publications

Characterization and Interrelations of One-Carbon Metabolites in Tissues, Erythrocytes, and Plasma in Mice with Dietary Induced Folate Deficiency

Characterization and Interrelations of One-Carbon Metabolites in Tissues, Erythrocytes, and Plasma in Mice with Dietary Induced Folate Deficiency

Disturbed biopterin and folate metabolism in the Qdpr‐deficient mouse

Current problems of food intake in young women in Japan: Their influence on female reproductive function

Contact Info

Product

Resources

About