Determination of folic acid by capillary zone electrophoresis with indirect chemiluminescence detection

Zhao, Tong; Lin, Haipeng; Li, Nan; Shi, Hongmei; Kang, Weijun; Xu, Xiangdong

doi:10.1039/d1ra02502c

Cited by 12 publications

(5 citation statements)

References 30 publications

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“…For that reason, only a few recent reviews and papers are cited here, which are dealing with general developments and applications of these detectors in separation methods. They include electrochemical detection alone [216,217] or in combination with MS [218], direct or indirect chemiluminescence [219], and electrochemiluminescence [220]. NMR spectroscopy is mostly offline combined with CE, especially for the structural analysis of analyte-selector complexes the strength of which was studied by CE [221][222][223].…”

Section: Other Detection Modesmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Other Detection Modesmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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“…26,27 Especially during pregnancy, FA supplements have been shown to effectively reduce the incidence and recurrence rates of neural tube defects. 28 Various analytical methods that have been designed for detecting FA include microbiological methods, 29 enzyme-linked immunosorbent assay, 30 high performance liquid chromatography, 31 capillary electrophoresis, 32 fluorescence, 33 chemiluminescence 34 and electrochemical methods. 35 However, most of these methods are restricted by the long detection cycle, poor sensitivity, expensive equipment and strong interference from sample pretreatment.…”

Section: Introductionmentioning

confidence: 99%

High luminescent N,S,P co-doped carbon dots for the fluorescence sensing of extreme acidity and folic acid

et al. 2023

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“…[1][2][3] Because of the significance of FA in maintaining human health, several methods have been developed for its determination. These methods include high-performance liquid chromatography (HPLC), 4 microbial assays, 5 spectrophotometry, 6 chemiluminescence, 7 colorimetry, 8 and fluorometry. 9 However, there is a growing interest in finding a reliable, simple, rapid, and efficient method for FA analysis.…”

Section: Introductionmentioning

confidence: 99%

Folic acid electrocatalytic oxidation on the carbon electrode surface modified with ferrocenyl grafted polymethylhydrosiloxane

Rahimpour

Zarenezhad

Teimuri‐Mofrad

2023

Applied Organom Chemis

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In this research, we synthesized ferrocenyl‐grafted polymethylhydrosiloxane (PMHS) through a hydrosilylation reaction between 3‐butenylferrocene and PMHS. The incorporation of the ferrocenyl derivative into the polymer backbone imparted electrochemical properties to the modified polymer. The modification of the polymer was confirmed by various techniques, including FT‐IR, 1H NMR, and UV–Vis spectroscopy. To prepare the new ferrocenyl polymer, we utilized cross‐linking agents, namely terephthaldehyde (TPA) and fresh egg white (FEW). The resulting polymer was drop‐coated onto a glassy carbon electrode (GCE) surface (5 μL). Electrochemical impedance spectroscopy (EIS) analysis confirmed the successful immobilization of the polymer on the GCE surface. Under physiological pH conditions (pH = 7), the Fc/Fc + couple facilitated the oxidation of folic acid (FA) at a potential of 0.40 V vs. the Ag/AgCl reference electrode. The GC/PMHS‐Fc/(TPA + FEW) electrode exhibited excellent stability, retaining 89% and 90% of its electrocatalytic activity after 500 cycles and 30 days of storage in a refrigerator, respectively. Thus, it shows promising potential for the determination of FA in biological fluids. The ferrocene‐modified electroactive polymer demonstrated satisfactory linear ranges for FA determination (5–150 μM) and a low limit of detection (LOD = 36 nM). These results highlight the effectiveness of the new polymer in detecting FA in biological samples.

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Determination of folic acid by capillary zone electrophoresis with indirect chemiluminescence detection

Abstract: A capillary electrophoresis method with on-line inhibited chemiluminescence detection was first used to determine folic acid.

Cited by 12 publications

References 30 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

High luminescent N,S,P co-doped carbon dots for the fluorescence sensing of extreme acidity and folic acid

Folic acid electrocatalytic oxidation on the carbon electrode surface modified with ferrocenyl grafted polymethylhydrosiloxane

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