2017
DOI: 10.1016/j.aca.2017.06.009
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Determination of formylated DNA and RNA by chemical labeling combined with mass spectrometry analysis

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Cited by 55 publications
(59 citation statements)
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“…With the advances of new techniques for whole-genome sequencing, carcinomas are discovered to be associated with modified nucleobases. Recently, researchers found that 5fU levels are about ten 5-formyldeoxyuridine per 10 6 nucleotides in human thyroid carcinoma tissues ( Jiang et al., 2017 ). So exploring the distribution of 5fU in cancer tissues might be vital for understanding the relationship between diseases and 5fU.…”
Section: Resultsmentioning
confidence: 99%
“…With the advances of new techniques for whole-genome sequencing, carcinomas are discovered to be associated with modified nucleobases. Recently, researchers found that 5fU levels are about ten 5-formyldeoxyuridine per 10 6 nucleotides in human thyroid carcinoma tissues ( Jiang et al., 2017 ). So exploring the distribution of 5fU in cancer tissues might be vital for understanding the relationship between diseases and 5fU.…”
Section: Resultsmentioning
confidence: 99%
“…The best reaction efficiencies (> 99%) were obtained with Girard's P reagent, GirP (2, Fig 2A). Investigations on DNA and RNA of cultured human cells (293T, MCF-7, and HeLa cells) and different rat tissues (liver, brain, kidney, and lung) using GirP reagent led to the simultaneous detection of six formylated nucleosides, among them the two new RNA modifications 5-formyl-2 0 -O-methylcytidine (5fCm) and 5-formyl-2 0 -O-methyluridine (5fUm) [72]. Owing to the strongly enhanced peak intensities of the labeled nucleosides in comparison to those of unlabeled nucleosides, and the consistently ionizable positively charged quaternary amino group in the labeling reagents, the detection limits were dramatically improved, for example, in the case of 5fC by 350-fold, and by 880-fold for 5fU.…”
Section: Chemical Labeling In Combination With Lc-esi-ms/ms Analysismentioning
confidence: 99%
“…The labeled nucleosides were online enriched by in-tube solid-phase microextraction (SPME) and then analyzed by elution to ultra-high performance liquid chromatography (UPLC) in combination with ESI-MS/MS. Relating to ongoing discussions on a potential role of cytosine and uracil modifications as early detection biomarkers for cancer diagnostics, the contents of formylated modifications in RNA and DNA of human thyroid carcinoma tissues were determined applying this approach [72]. Thus, the sensitivity upon labeling with GirP was distinctly better than that of BDAPE-labeled nucleosides (see above).…”
Section: Chemical Labeling In Combination With Lc-esi-ms/ms Analysismentioning
confidence: 99%
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