Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography

Abudayeh, Zead Helmi; Azzam, Khaldun M. Al; Naddaf, Ahmad; Karpiuk, Uliana; Кисличенко, В. С.

doi:10.15171/apb.2015.079

Cited by 16 publications

(7 citation statements)

References 14 publications

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“…From the results, it could be observed that at 150 °C, the amount of escins decreases with increasing extraction time, while at 200 °C after 5 min, they are completely degraded. At 150 °C and 5 min, the amount of escins extracted from seeds was 33.11 mg g -1 of material, which shows that the amount of escins in seed extracts obtained by subcritical water extraction was almost the same as that obtained by extraction with methanol (34.9 mg g -1 for the year of 2014) published by Abudayeh et al 10 . Chen et al determined four main saponins (escin Ia, escin Ib, isoescin Ia, isoescin Ib) from a different variety of horse chestnut (Aesculus chinensis) using accelerated solvent extraction.…”

Section: Triterpene Saponins Coumarin Glycosides Phenolic Acids and Furfuralssupporting

confidence: 62%

“…As can be seen from the chromatograms, the detected peaks represent escins, such as escin Ia (1), escin Ib (2), isoescin Ia (3) and isoescin Ib (4). The peaks were identified based on the data published by Abudayeh et al 10 The amounts of chlorogenic acid, neochlorogenic acid and galic acid extracted from different parts of horse chestnut are presented in Table V. Chlorogenic acid was detected in higher amounts only in extracts from bark, while in extracts from other chestnut parts, it was present only in trace amounts.…”

Section: Triterpene Saponins Coumarin Glycosides Phenolic Acids and Furfuralsmentioning

confidence: 99%

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Subcritical water extraction of horse chestnut (Aesculus hippocastanum) tree parts

Gagić

Knez

Škerget

2021

JSCS

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Subcritical water extraction of horse chestnut (Aesculus hippocastanum) parts, such as seeds, seed shell, bark and leaves has been performed in a batch reactor at temperatures of 150, 200 and 250?C and extraction times of 5, 15 and 30 min. The obtained extracts were analyzed by spectrophotometric methods to determine the total phenols, total carbohydrates and antioxidant activity. Furthermore, the compounds detected in the extracts, such as triterpene saponins (escins), coumarin glycosides (esculin and fraxin), phenolic compounds (chlorogenic, neochlorogenic and gallic acids) and furfurals (5-hydroxymethyfurfural, furfural, and 5-methylfufrual) were quantified using HPLC. High amount of escins was obtained in the seed extracts, while the highest amounts of esculin and fraxin were obtained in bark extracts. The total phenol content was the highest in shell and bark extracts, which implies that these extracts gave the highest antioxidant activity.

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Section: Triterpene Saponins Coumarin Glycosides Phenolic Acids and Furfuralssupporting

confidence: 62%

Section: Triterpene Saponins Coumarin Glycosides Phenolic Acids and Furfuralsmentioning

confidence: 99%

Subcritical water extraction of horse chestnut (Aesculus hippocastanum) tree parts

Gagić

Knez

Škerget

2021

JSCS

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“…In this study, three different nanovesicles (CL, ECL, and escinosomes EL), based on P90G, CHOL, and ESN, were developed and optimized. In particular, ESN, a natural mixtures of structurally correlated pentacyclic triterpene saponins [49], largely employed as a vasoprotective anti-inflammatory, anti-edematous and antinociceptive agent, was used at a dose of 5 mg/mL to formulate ECL and EL nanovesicles.…”

Section: Discussionmentioning

confidence: 99%

Development and Percutaneous Permeation Study of Escinosomes, Escin-Based Nanovesicles Loaded with Berberine Chloride

et al. 2019

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Escin is a natural saponin, clinically used for the anti-edematous and anti-inflammatory effects. The aim of the study was to explore the possibility of converting escin into vesicle bilayer-forming component. The hyaluronidase inhibition activity of escin was evaluated after its formulation in escinosomes. Berberine chloride, a natural quaternary isoquinoline alkaloid isolated from several medicinal plants that is traditionally used for various skin conditions was loaded in the vesicles. The developed nanovesicles were characterized in terms of diameter, polydispersity, ζ-potential, deformability, recovery, encapsulation efficiency, stability, and release kinetics. Nanovesicle permeation properties through artificial membranes and rabbit ear skin were investigated using skin-PAMPATM and Franz cells were also evaluated. Escinosomes, made of phosphatidylcholine and escin, were loaded with berberine chloride. These nanovesicles displayed the best characteristics for skin application, particularly optimal polydispersity (0.17) and deformability, high negative ζ-potential value, great encapsulation efficiency (about 67%), high stability, and the best release properties of berberine chloride (about 75% after 24 h). In conclusion, escinosomes seem to be new vesicular carriers, capable to maintain escin properties such as hyaluronidase inhibition activity, and able to load other active molecules such as berberine chloride, in order to enhance or expand the activity of the loaded drug.

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“…The highest concentration of most chemicals is in the seeds, particularly the cotyledons but they are also found in the fruit, bark, leaves and buds (Bombardelli, Morazzoni, & Griffini, ; Otajagić, Pinjić, Ćavar, Vidic, & Maksimović, ) and in embryonic callus tissue (Profumo, Caviglia, Gastaldo, & Dameri, ). Abudayeh, Al Azzam, Naddaf, Karpiuk, and Kislichenko () investigated seeds from Poland and found lower levels of saponins in the seed coat (0.19–0.32 g/kg) than the seed's endosperm (34.9–52.05 g/kg). The levels decreased by >30% in the endosperm and >40% in the skin when stored air‐dried for 2 years.…”

Section: Structure and Physiologymentioning

confidence: 99%

Biological Flora of the British Isles:Aesculus hippocastanum

et al. 2019

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This account presents information on all aspects of the biology of Aesculus hippocastanum L. (horse‐chestnut) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of the British Isles: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history and conservation. Aesculus hippocastanum is a large deciduous tree native to the Balkan Peninsula. Native populations are small (<10,000 trees total) and apparently in decline, but the tree has been widely planted in gardens and streets across Europe and other temperate areas from the 17th century onwards. It was voted the UK's favourite tree in a 2017 poll. As a British neophyte, it is occasionally naturalised in open wooded habitats. Horse‐chestnut is renowned for the beauty of its large (up to 30 cm long), upright panicles of white flowers, and for the large seeds (up to 42 g each) used in the formerly common children's game of “conkers.” More recently, the triterpene glycosides, extractable from various plant parts but especially the seeds, have been widely used in medicine. In much of Europe, horse‐chestnut is affected by chestnut bleeding canker (caused by Pseudomonas syringae pv. aesculi), the horse‐chestnut leaf miner Cameraria ohridella and the leaf blotch fungus Guignardia aesculi. The canker is likely to lead to death of <10% individuals, but seeds of plants infested with the leaf miner are 40%–50% smaller, which may affect long‐term establishment in non‐planted areas.

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Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography

Cited by 16 publications

References 14 publications

Subcritical water extraction of horse chestnut (Aesculus hippocastanum) tree parts

Subcritical water extraction of horse chestnut (Aesculus hippocastanum) tree parts

Development and Percutaneous Permeation Study of Escinosomes, Escin-Based Nanovesicles Loaded with Berberine Chloride

Biological Flora of the British Isles:Aesculus hippocastanum

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