Determination of Free Amino Acids in Bee Pollen by Liquid Chromatography with Fluorescence Detection

Ares, Ana M.; Martín, Margarita; Toribio, Laura; Bernal, José

doi:10.1007/s12161-022-02281-8

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…Trueness was evaluated by the mean recoveries (as a measure of trueness) calculated by comparing the measured concentrations in spiked samples (see Section 2.2 ) and theoretical concentrations. Mean recoveries ranged from 85% to 105% (%RSD < 15%) in all cases (see results for some amino acids in Supplementary Materials, Table S1 ), which are comparable to previous works [ 5 , 17 , 24 , 26 ].…”

Section: Resultssupporting

confidence: 88%

“…As can be seen in Table 1 and Table 2 , the values were lower in all cases when GC-MS was employed. In addition, the LOQs obtained were like those reported in previous studies [ 5 , 17 , 26 ].…”

Section: Resultssupporting

confidence: 85%

“…As chromatographic analysis was faster with GC-MS, the tests were initially performed with this technique. Free amino acids have generally been extracted from bee pollen with ultrapure water and ethanol [ 26 ]. Consequently, we tested both solvents.…”

Section: Resultsmentioning

confidence: 99%

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Fast Chromatographic Determination of Free Amino Acids in Bee Pollen

Martín-Gómez

Salahange

Tapia

et al. 2022

Foods

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The consumption of bee pollen has increased in the last few years due to its nutritional and health-promoting properties, which are directly related to its bioactive constituents, such as amino acids. Currently, there is great interest in understanding the role of these in bee products as it provides relevant information, e.g., regarding nutritional value or geographical and botanical origins. In the present study, two fast chromatographic methods were adapted based on commercial EZ:faast™ kits for gas chromatography-mass spectrometry and liquid chromatography–mass spectrometry for determining free amino acids in bee pollen. Both methods involved the extraction of amino acids with water, followed by a solid phase extraction to eliminate interfering compounds, and a derivatization of the amino acids prior to their chromatographic separation. The best results in terms of run time (<7 min), matrix effect, and limits of quantification (3–75 mg/kg) were obtained when gas chromatography–mass spectrometry was employed. This latter methodology was applied to analyze several bee pollen samples obtained from local markets and experimental apiaries. The findings obtained from a statistical examination based on principal component analysis showed that bee pollen samples from commercial or experimental apiaries were different in their amino acid composition.

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Section: Resultssupporting

confidence: 88%

Section: Resultssupporting

confidence: 85%

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Fast Chromatographic Determination of Free Amino Acids in Bee Pollen

Martín-Gómez

Salahange

Tapia

et al. 2022

Foods

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“…Despite its composition variability BP contains all the essential amino acids (AAs) needed by the human body, as well as others used to build proteins in humans [ 47 ]. However, this does not mean that all types of pollen contain all these AAs.…”

Section: Resultsmentioning

confidence: 99%

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

Kacemi,

Campos

2023

Nutrients

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The emphasis on healthy nutrition is gaining a forefront place in current biomedical sciences. Nutritional deficiencies and imbalances have been widely demonstrated to be involved in the genesis and development of many world-scale public health burdens, such as metabolic and cardiovascular diseases. In recent years, bee pollen is emerging as a scientifically validated candidate, which can help diminish conditions through nutritional interventions. This matrix is being extensively studied, and has proven to be a very rich and well-balanced nutrient pool. In this work, we reviewed the available evidence on the interest in bee pollen as a nutrient source. We mainly focused on bee pollen richness in nutrients and its possible roles in the main pathophysiological processes that are directly linked to nutritional imbalances. This scoping review analyzed scientific works published in the last four years, focusing on the clearest inferences and perspectives to translate cumulated experimental and preclinical evidence into clinically relevant insights. The promising uses of bee pollen for malnutrition, digestive health, metabolic disorders, and other bioactivities which could be helpful to readjust homeostasis (as it is also true in the case of anti-inflammatory or anti-oxidant needs), as well as the benefits on cardiovascular diseases, were identified. The current knowledge gaps were identified, along with the practical challenges that hinder the establishment and fructification of these uses. A complete data collection made with a major range of botanical species allows more robust clinical information.

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“…Pollen grain micromorphology shows that they contain various chemicals such as exines (Abbas et al, 2022). These chemicals can be used in different ways such as delivering medicine, creating new materials, acting as templates, making carbon microspheres and helping in protein chromatography (Ares et al, 2022; Lu et al, 2014; Majeed et al, 2022). High‐performance thin‐layer liquid chromatography (HPTLC) is a chromatographic technique that has several advantages when compared with other techniques (Ristivojević et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Chromatographic authentication of botanical origin: Herbaceous pollen profiling with HPLC, HPTLC and GC–MS analysis

Aziza,

Khaydarov,

Zafar

et al. 2024

Biomedical Chromatography

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This study describes a robust chromatographic authentication methodology for herbaceous pollen, employing gas chromatography–mass spectrometry (GC–MS), high‐performance liquid chromatography (HPLC) and high‐performance thin liquid chromatography (HPTLC) protocols. The comprehensive profiling of organic compounds not only distinguishes between different botanical sources but also establishes a reliable framework for quality control and assessment of herbaceous pollen authenticity. Traces of quercetin were detectable using HPTLC in Chaenomeles japonica, and the composition of the mobile phase led to distinct phenolic acid tracks in the extracts of free phenolic compounds. In Lonicera nummulariifolia, prominent chlorogenic acid signal and traces of 3,4‐dihydroxybenzoic acid were identified, along with the presence of vanillic, trans‐ferulic, p‐coumaric and p‐hydroxybenzoic and sinapic as phenolic acid standards. The HPLC chromatogram identified six peaks representing bioactive phenolic compounds such as gallic acid measuring 5.89 ± 0.56 mg g−1, hydroxybenzoic acid 2.39 ± 0.78 mg g−1 and caffeic acid 2.83 ± 0.11 mg g−1. The combined use of GC–MS, HPTLC and HPLC techniques provides a powerful and reliable means of authenticating the botanical origin of herbaceous pollen, offering valuable insights for quality control and ensuring the accuracy of botanical source identification.

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Determination of Free Amino Acids in Bee Pollen by Liquid Chromatography with Fluorescence Detection

Cited by 7 publications

References 22 publications

Fast Chromatographic Determination of Free Amino Acids in Bee Pollen

Fast Chromatographic Determination of Free Amino Acids in Bee Pollen

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

Chromatographic authentication of botanical origin: Herbaceous pollen profiling with HPLC, HPTLC and GC–MS analysis

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