Determination of Free Fatty Acids in Human Bile by High-Performance Liquid Chromatography

Hori, Yasushi; Nakamura, Kazutoshi; Yamamoto, Masahiro; Shimada, Kenji; Nakadaira, Hiroto; Shibuya, Norio; Endoh, Kazuo; Ogoshi, Kazuei

doi:10.1177/000456329803500213

Cited by 8 publications

(2 citation statements)

References 7 publications

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“…Pertaining to the structural modification of phospholipids with exogenously acquired fatty acids, our results using thin-layer chromatography and UPLC-MS provide a convincing argument for the environmental relevance of this phenomenon. First, the concentration of fatty acids used in this study (300 M) is within the expected range of estimated physiologically and environmentally relevant values (44)(45)(46). Second, the combination of altered migration patterns in TLC and divergent UPLC chromatogram profiles strongly suggest wholesale changes in the membrane phospholipid composition.…”

Section: Discussionsupporting

confidence: 59%

Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species

Moravec

Siv

Hobby

et al. 2017

Appl Environ Microbiol

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The pathogenic species (, , and) represent a constant threat to human health, causing foodborne and skin wound infections as a result of ingestion of or exposure to contaminated water and seafood. Recent studies have highlighted 's ability to acquire fatty acids from environmental sources and assimilate them into cell membranes. The possession and conservation of such machinery provokes consideration of fatty acids as important factors in the pathogenic lifestyle of species. The findings here link exogenous fatty acid exposure to changes in bacterial membrane phospholipid structure, permeability, phenotypes associated with virulence, and consequent stress responses that may impact survival and persistence of pathogenic species. Polyunsaturated fatty acids (PUFAs) (ranging in carbon length and unsaturation) supplied in growth medium were assimilated into bacterial phospholipids, as determined by thin-layer chromatography and liquid chromatography-mass spectrometry. The incorporation of fatty acids variably affected membrane permeability, as judged by uptake of the hydrophobic compound crystal violet. For each species, certain fatty acids were identified as affecting resistance to antimicrobial peptide treatment. Significant fluctuations were observed with regard to both motility and biofilm formation following growth in the presence of individual PUFAs. Our results illustrate the important and complex roles of exogenous fatty acids in the membrane physiology and virulence of a bacterial genus that inhabits aquatic and host environments containing an abundance of diverse fatty acids. Bacterial responses to fatty acids include, but are not limited to, degradation for metabolic gain, modification of membrane lipids, alteration of protein function, and regulation of gene expression. species exhibit significant diversity with regard to the machinery known to participate in the uptake and incorporation of fatty acids into their membranes. Both aquatic and host niches occupied by are rife with various free fatty acids and fatty acid-containing lipids. The roles of fatty acids in the environmental survival and pathogenesis of bacteria have begun to emerge and are expected to expand significantly. The current study demonstrates the responsiveness of ,, and to exogenous PUFAs. In addition to phospholipid remodeling, PUFA assimilation impacts membrane permeability, motility, biofilm formation, and resistance to polymyxin B.

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Section: Discussionsupporting

confidence: 59%

Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species

Moravec

Siv

Hobby

et al. 2017

Appl Environ Microbiol

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“…In summary, the Chilean bile had a higher mutagenic activity level than the Japanese bile. In a Japanese study, we recently discovered that the presence of free fatty acids can inhibit the mutagenic activity of blue rayontreated bile samples (28,29). We proceeded to perform a comparative study between Japan and Chile.…”

Section: ) Determinants Of Gbc Distributionmentioning

confidence: 99%

Epidemiological studies on the distribution and determinants of biliary tract cancer

Yamamoto

2003

Environ Health Prev Med

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With the help of my colleagues, I have been conducting epidemiological studies on biliary tract cancer (BTC), including gallbladder cancer (GBC) and extrahepatic bile duct cancer (BDC), in Japan and Chile for about 19 years. Clustered areas with high mortality rates, especially for female GBC were found to correspond with places or prefectures in Japan that were famous for rice production. The roles of known risk factors, such as gallstones and cholecystitis, were examined, but no single factor was implicated in the high mortality rates for GBC in these areas. A working hypothesis, called the "rice production hypothesis" was formulated; this initial hypothesis was replaced by a new multifactorial causation hypothesis: GBC is more likely to occur in individuals with a genetic susceptibility and a past history of gallstones or cholecystitis who are exposed to geographically specific environmental factors, such as agricultural chemicals. On the basis of various analytical studies, it is concluded that a certain agricultural chemical was responsible for the occurrence of GBC. At the time of writing, no evidence has been obtained to disprove our hypothesis. We have also conducted international collaborative studies in Chile, which has the highest mortality rate for GBC in the world. Bile from Chileans was found to have a higher mutagenic activity than that from Japanese subjects; Chileans with a history of constipation or a habit of consuming red chilli pepper had a high risk of developing GBC, if they also had gallstone(s). The presence of a regional difference in p53 mutagenesis was also observed.

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Free fatty acid concentrations in gallbladder bile collected from Chilean patients with gallstones

Tsuchiya

Serra

Hori

et al. 2006

Clinical Biochemistry

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Determination of Free Fatty Acids in Human Bile by High-Performance Liquid Chromatography

Cited by 8 publications

References 7 publications

Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species

Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species

Epidemiological studies on the distribution and determinants of biliary tract cancer

Free fatty acid concentrations in gallbladder bile collected from Chilean patients with gallstones

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