Determination of free phenolic acids and antioxidant capacity of methanolic extracts obtained from leaves and flowers of camel thorn (<i>Alhagi maurorum</i>)

Laghari, Abdul Hafeez; Memon, Ayaz Ali; Memon, Shahabuddin; Nelofar, Aisha; Khan, Khalid M.; Yasmin, Arfa

doi:10.1080/14786419.2010.538846

Cited by 46 publications

(36 citation statements)

References 5 publications

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“…This is the case with carotenoids that are present in high concentrations in tucumã fruit as described in previous studies and in our results (Morrison et al, 1995;Sreevidja and Mehrotra (2003); Laghari et al, 2012). De Rosso and Mercadante (2007) described 24 carotenoids of which 21 were chemically identified in tucumã fruit.…”

Section: Discussionsupporting

confidence: 78%

“…After one hour, H 2 O 2 was added in the cultures. The cells were cultured in a 96-well microplate at an initial density of 2 Â 10 5 cells for 72 h at 37°C in a 5% humidified CO 2 atmosphere (Laghari et al, 2012). In addition, the cells were counted, centrifuged for 10 min at 2000g, and transferred to a new culture media containing H 2 O 2 treatments with and without tucumã extracts for one hour.…”

Section: Blood Collection and Lymphocyte Culturementioning

confidence: 99%

“…Chromatographic analyses were carried out following the method described by Laghari et al (2012), with slight modifications. The analyses were performed using a C18 column (4.6 mm Â 250 mm) packed with 5 lm diameter particles.…”

Section: Chemical Characterisation Of Tucumã Extractsmentioning

confidence: 99%

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Tucumã fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes

et al. 2015

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This study quantifies the bioactive molecules in and determines the in vitro protective effect of ethanolic extracts isolated from the peel and pulp of tucumã (Astrocaryum aculeatum, Mart.), an Amazonian fruit rich in carotenoids. The cytoprotective effect of tucumã was evaluated in lymphocyte cultures exposed to H2O2 using spectrophotometric, fluorimetric, and immunoassay assays. The results confirmed that tucumã pulp extract is rich in β-carotene and quercetin, as previously described in the literature. However, high levels of these compounds were also found in tucumã peel extract. The extracts also contained significant amounts rutin, gallic acid, caffeic acid, and chlorogenic acid. Despite quantitative differences in the concentration of these bioactive molecules, both extracts increased the viability of cells exposed to H2O2 in concentrations ranging from 300 to 900 μg/mL. Caspases 1, 3, and 8 decreased significantly in cells concomitantly exposed to H2O2 and these extracts, indicating that tucumã cryoprotection involves apoptosis modulation.

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Section: Discussionsupporting

confidence: 78%

Section: Blood Collection and Lymphocyte Culturementioning

confidence: 99%

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Tucumã fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes

et al. 2015

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“…The HPLC analyses were carried out under gradient conditions using the C18 column (4.6 mm · 250 mm) packed with 5 lm diameter particles; the mobile phase was water containing 2% acetic acid (A) and methanol (B), and the composition gradient was 5% of B for 2 min, then changed to obtain 25%, 40%, 50%, 60%, 70%, and 100% B at 10, 20, 30, 40, 50, and 80 min, respectively, following the method described by Laghari et al, 14 with slight modifications. The lyophilized tucuma peel and pulp extracts were analyzed and dissolved in ethanol at a concentration of 3 mg/mL.…”

Section: Tucuma Extract Production and Chemical Characterizationmentioning

confidence: 99%

TheIn VitroGenotoxic Effect of Tucuma (Astrocaryum aculeatum), an Amazonian Fruit Rich in Carotenoids

Filho

Sagrillo²,

Garcia³

et al. 2013

Journal of Medicinal Food

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Tucuma (Astrocaryum aculeatum) is an Amazonian fruit that presents high levels of carotenoids and other bioactive compounds such as quercetin. The extracts of tucuma peel and pulp present strong antioxidant activity which illustrate an elevated concentration that causes cytotoxic effects in human peripheral blood mononuclear cells (PBMCs). This study performed additional investigations to analyze the potential genotoxic effects of the tucuma extracts on PBMCs. The genotoxicity was evaluated by DNA fragmentation, Comet assay, and chromosomal instability G-band assays. The acute tucuma extract treatment showed genoprotective effects against DNA denaturation when compared with untreated PBMC cells. However, in the experiments with 24 and 72 h treatments to tucuma treatments, we observed low genotoxicity through a concentration of 100 μg/mL, some genotoxic effects related to intermediary concentrations (100-500 μg/mL), and more pronounced genotoxic effects on higher tucuma extract concentrations. After 24 h of treatment, the reactive oxygen species were similar among treatments and PBMC control groups. However, the caspase-1 activity related to the apoptosis and pyroptosis process increased significantly in higher tucuma concentrations. In summary, tucuma extracts, despite their higher antioxidant content and antioxidant activity, would present PBMCs genotoxic effects that are dependent on concentration and time exposition. These results need to be considered in future in vitro and in vivo studies of tucuma effects.

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“…[21] The leaf extract has higher antioxidant potential than the flower extract due to its higher phenolic contents. [22] Three important antioxidant flavonoids have been isolated from A. maurorum which include isorhamnetin-3-O-[-alpha-l-rhamnopyranosyl- (1-3)]- beta- D-glucopyranoside, 3’-O-methylorobol and Quercetin 3-O-beta-d-glucopyranoside. [37]…”

Section: Antioxidant Effectsmentioning

confidence: 99%

A systematic and mechanistic review on the phytopharmacological properties of Alhagi species

Asghari¹,

Fallah²,

Moloudizargari³

et al. 2016

Ancient Sci Life

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Alhagi species are well known in Iran (locally known as Khar Shotor) and other parts of Asia as a popular folk medicine. Recent research has shown extensive pharmacological effects of these species. This paper is a comprehensive review of the phytopharmacological effects and traditional uses of Alhagi species and their active constituents with special attention to the responsible mechanisms, effective dosages and routes of administration. The Alhagi species studied in this paper include: A. maurorum, A. camelorum, A. persarum, A. pseudoalhagi, and A. kirgisorum. In order to include all the up to date data, the authors went through several databases including the Web of Science, Embase, etc. The findings were critically reviewed and sorted on the basis of relevance to the topic. Tables have been used to clearly present the ideas and discrepancies were settled through discussion. Alhagi species have significant biomedical properties which can be exploited in clinical use. Proantocyanidin isolated from A. pseudoalhagi has significant biochemical effects on blood factors. Among Alhagi species, A. camelorum and A. maurorum possess the highest anti-microbial activity. Most of the effects observed with A. maurorum are dose-dependent. This paper indicates with emphasis that Alhagi species are safe and rich sources of biologically active compounds with low toxicity. Since DNA damage has been observed following the ingestion of specific concentrations of A. pseudalhagi, care should be taken during administration of the plant for therapeutic use. Further studies are required to confirm the safety and quality of these plants to be used by clinicians as therapeutic agents.

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Determination of free phenolic acids and antioxidant capacity of methanolic extracts obtained from leaves and flowers of camel thorn (Alhagi maurorum)

Cited by 46 publications

References 5 publications

Tucumã fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes

Tucumã fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes

TheIn VitroGenotoxic Effect of Tucuma (Astrocaryum aculeatum), an Amazonian Fruit Rich in Carotenoids

A systematic and mechanistic review on the phytopharmacological properties of Alhagi species

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