Determination of Free Radical Scavenging Activity of Plant Extracts Through DPPH Assay: An EPR and UV–Vis Study

Sanna, Daniele; Delogu, Giovanna; Mulas, Maurizio; Schirra, M.; Fadda, Angela

doi:10.1007/s12161-011-9306-1

Cited by 99 publications

(69 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The antioxidant activity of the fruit juices was also evaluated by DPPH according to Sanna et al (2012), with some modifications, and ABTS methods (Re et al 1999). Results were expressed as μmoles equivalents of AA/mL of juice The detailed methodologies were reported in Supplementary data (S-2.4).…”

Section: Evaluation Of Antioxidant Capacity With Reference Methodsmentioning

confidence: 99%

Simultaneous amperometric detection of ascorbic acid and antioxidant capacity in orange, blueberry and kiwi juice, by a telemetric system coupled with a fullerene- or nanotubes-modified ascorbate subtractive biosensor

Barberis

Spissu

Fadda

et al. 2015

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

Section: Evaluation Of Antioxidant Capacity With Reference Methodsmentioning

confidence: 99%

Simultaneous amperometric detection of ascorbic acid and antioxidant capacity in orange, blueberry and kiwi juice, by a telemetric system coupled with a fullerene- or nanotubes-modified ascorbate subtractive biosensor

Barberis

Spissu

Fadda

et al. 2015

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

“…The DPPH assay for antioxidant activity, proposed more than five decades ago, is based on the ability of the antioxidant to reduce the initial concentration of the DPPH radical, a process which is associated with turning the colour of the solution from purple (colour of the DPPH radical) to yellow (colour of the corresponding hydrazine DPPH-H) (Sanna et al, 2012;Brand-Williams et al, 1995;Xie & Schaich, 2014). Although the method of antioxidant evaluation using DPPH is apparently very simple, a number of studies have highlighted the influence of various factors (pH, temperature, solvent nature the relative concentration of DPPH and antioxidants tested) on the reaction mechanism and rate between DPPH and antioxidant (Xie & Schaich, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Hence, the presence of antioxidants limits the negative effect of ROS in biological systems. On the other hand, the oxidative processes occurring in food, cosmetic or polymeric materials responsible for the degradation of these systems are often limited by the addition of synthetic antioxidants such as butylated hydroxytoluene or butylated hydroxyanisole (Sanna et al, 2012). The extensive use of synthetic antioxidants has raised concerns about their safety, as they lead to the formation of hazardous quinones.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of rosemary extracts in solution and embedded in polymeric systems

et al. 2015

View full text Add to dashboard Cite

The rosemary extract was encapsulated in polyethylene or in covalently-based network gels. The covalent gels were obtained by the reaction of isocyanate end-capped polyethylene glycol (PEG) with β-cyclodextrin or glycerol. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to evaluate the antioxidant activity (AA) of rosemary extract entrapped in polymeric structures and in ethanol or water solutions. The AA of the rosemary extract was determined using a DPPH radical for samples prepared in ethanol, and a water-soluble derivative, the sulphonated DPPH radical (DPPH-SO3Na), for the rosemary extract in water. Formulation of the rosemary extract in polymeric gels ensures a rapid release which determines the AA values similar to those in solution.

show abstract

“…(19,20) In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) RSA assay, in which the same sample was evaluated by using both EPR and UV-visible spectrophotometry, it was suggested that absorption by the decomposition products of the antioxidants accounts for the difference in the outcomes of the two techniques. (21) If necessary, turbid samples, which are not extracted, can be measured by the EPR based assay reported herein. In conclusion, this RSA assay employing EPR spectroscopy is highly useful for the determination of ROO • scavenging activities of colored or turbid food samples.…”

Section: Discussionmentioning

confidence: 99%

2,2^|^apos;-Azobis(isobutyronitrile)-derived alkylperoxyl radical scavenging activity assay of hydrophilic antioxidants by employing EPR spin trap method

Kohri

Fujii

2013

J. Clin. Biochem. Nutr.

View full text Add to dashboard Cite

As interest in the study of antioxidant intake from foods and other agricultural products increases, methods for performing radical scavenging activity assays based on the electron paramagnetic resonance spectroscopic method, in which there is no interference from the sample color and turbidity, are required. In this study, we have developed a rapid and simple electron paramagnetic resonance based assay to evaluate the alkylperoxyl radical scavenging activity of several antioxidants. The alkylperoxyl radical species was generated by the photolysis of azo-radical initiator 2,2'-azobis(isobutyronitrile), in which the radical generation rate and period were controlled by the illumination light. The relative alkylperoxyl radical scavenging activity was obtained by a simple formula of competing reaction of antioxidant and spin trap toward the oxygen radical. The scavenging activities toward alkylperoxyl radical and alkoxy radical species were evaluated in six antioxidants. Although quercetin showed the highest activity toward both radicals, the order of the relative activities in the other antioxidants was different mutually between the alkylperoxyl radical and the alkoxyl radical. This alkylperoxyl radical scavenging activity assay based on electron paramagnetic resonance spectroscopy is useful for evaluation of colored and turbid food samples.

show abstract

Determination of Free Radical Scavenging Activity of Plant Extracts Through DPPH Assay: An EPR and UV–Vis Study

Cited by 99 publications

References 21 publications

Simultaneous amperometric detection of ascorbic acid and antioxidant capacity in orange, blueberry and kiwi juice, by a telemetric system coupled with a fullerene- or nanotubes-modified ascorbate subtractive biosensor

Simultaneous amperometric detection of ascorbic acid and antioxidant capacity in orange, blueberry and kiwi juice, by a telemetric system coupled with a fullerene- or nanotubes-modified ascorbate subtractive biosensor

Antioxidant activity of rosemary extracts in solution and embedded in polymeric systems

2,2^|^apos;-Azobis(isobutyronitrile)-derived alkylperoxyl radical scavenging activity assay of hydrophilic antioxidants by employing EPR spin trap method

Contact Info

Product

Resources

About