Determination of Fructosyl Amino Acids and Fructosyl Peptides in Protease-digested Blood Sample by a Flow-Injection System with an Enzyme Reactor

Glycated proteins are generated by binding of glucose to the proteins in blood stream through a nonenzymatic reaction. Hemoglobin A1c (HbA1c) is a glycated protein with glucose at the N‐terminal of β‐chain. HbA1c is extensively used as an indicator for assessing the blood glucose concentration in diabetes patients. There are different conventional clinical methods for the detection of HbA1c. However, enzymatic detection method has newly obtained great attention for its high precision and cost‐effectiveness. Today, fructosyl peptide oxidase (FPOX) plays a key role in the enzymatic measurement of HbA1c, and different companies have marketed HbA1c assay systems based on FPOX. Recent investigations show that FPOX could be used in assaying HbA1 without requiring HbA1c primary digestion. It could also be applied as a biosensor for HbA1c detection. In this review, we have discussed the recent improvements of FPOX properties, different methods of FPOX purification, solubility, and immobilization, and also the use of FPOX in HbA1c biosensors.

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1-Amino-1-deoxy-d-fructose (“fructosamine”) and its derivatives

Mossine,

Mawhinney

2023

Advances in Carbohydrate Chemistry and Biochemistry

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Determination of Fructosyl Amino Acids and Fructosyl Peptides in Protease-digested Blood Sample by a Flow-Injection System with an Enzyme Reactor

Cited by 8 publications

References 29 publications

Enzymes in Flow Injection Analysis

Enzymes in Flow Injection Analysis

A comprehensive review on fructosyl peptide oxidase as an important enzyme for present hemoglobin A1c assays

1-Amino-1-deoxy-d-fructose (“fructosamine”) and its derivatives

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