Determination of galanthamine in Bulbus Lycoridis Radiatae by coupling capillary electrophoresis with end‐column electrochemiluminescence detection

Abstract

A novel method for the determination of galanthamine (GAL) in Bulbus Lycoridis Radiatae has been developed based on coupling CE with an end-column tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence (ECL). Parameters affecting CE separation and ECL detection were investigated and optimized. Baseline separation of GAL from other components in the Bulbus Lycoridis Radiatae sample was achieved with an 18 mmol/L phosphate running buffer at pH 9.0. Under the optimized conditions: 12 kV CE-separation voltage, ECL detection potential at 1.25 V with 5 mmol/L Ru(bpy)(3)(2+) and 50 mmol/L phosphate buffer at pH 7.5 in the detection reservoir, the linear range of GAL concentration was from 0.8 ng/mL to 2 microg/mL, whereas the detection limit was 0.25 ng/mL (S/N=3). The proposed method was successfully demonstrated for the determination of GAL in Bulbus Lycoridis Radiatae.

Applications of Electrochemiluminescence

“…Pesticides [114], acephate and dimethoate, were determined using a composite electrode (a TBR-multi-walled carbon nanotube paste electrode) with coreagent TPA [115]. Other literature revealed a rapid method for the determination of galanthamine (in Bulbus lycoridis radiatae) [116]. For sensitive determination of verticine and verticinone in Bulbus fritillariae, CE/Ru(bpy) 3 2+ ECL system with the assistance of ionic liquids was successfully established.…”

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

“…UV detection is obviously still the most popularly used detection strategy but MS detection is gaining more and more ground 65, 138–149. Also used were ECL 150–158 and LIF 152, 159–164.…”

“…There was a good number of CE investigations of pharmaceuticals/herbal product compounds 138, 150–153, 157, 159, 166–169, 171, 172, 176, 178–180, 182, 186, 187, 341, 357–375 during this review period. Mostly performed in MEKC mode, these included the determination of biogenic amines (toxicological‐risk agents) 364, toxic pyrrolizidine alkaloids in G ynura segenum (a health‐risk plant species) 168, diterpenoids in Salvia species (a herbal remedy and food flavor) 166, statins (cholesterol‐lowering drugs, some of which are naturally occurring) 365 in drug products.…”

CE and CEC analysis of phytochemicals in herbal medicines

“…Ultrasonic extraction was the most frequently used method due to its efficiency and easy manipulation. Indeed, it was employed in about two‐thirds (180 out of 271 articles) of cited references (Supporting Information Tables 1–7) 4–271. Other conventional extraction methods included reflux 6, 14, 15, 21, 45, 74, 84, 87, 97, 117, 121, 123, 131, 134, 157, 162, 164, 169, 171, 172, 176, 179, 182–184, 205, 212, 227, 235, 236, 259, Soxhlet extraction 23, 89, 91, 128, 131, 244, heating extraction 22, 39, 51...…”