Determination of gender from dental pulp by identification of Barr bodies

Bhardwaj, Nandini; Nangia, Rajat; Puri, Abhiney; Bhat, Nitish; Wadhwan, Vijay; Gupta, Hitesh

doi:10.4103/jomfp.jomfp_250_22

Cited by 4 publications

(1 citation statement)

References 16 publications

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“…A recent study by Bhardwaj N et al ,[ 22 ] stated that Barr bodies were better analysed and appreciated in histopathological sections than in cytopathological technique.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of cheiloscopy, pulpal tissue and fingerprint for gender identification

Nayak,

Solanki

et al. 2023

Journal of Oral and Maxillofacial Pathology

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Background: The most important step in identifying an unknown person is determining one’s gender and as a dentist, the oral tissues are potential sources of information in this aspect. A study was carried out to assess and evaluate the accuracy of cheiloscopy, pulp tissue, and fingerprints in determining gender. Material and Methods: A study comprising of 160 individuals (80 males and 80 females) was conducted. After obtaining informed written consent and recording their bio-data; lip prints, and fingerprints were recorded. The patients’ extracted tooth was collected, their pulp extirpated, for assessment of the Barr body. Results: We found that every lip pattern was unique and hence can be used to identify an unknown individual. The occurrence of the Barr body was determined, and all female samples were found to be positive for the existence of the Barr body. In fingerprint patterns, a significant difference was noted between both sexes with ulnar loops and whorl patterns only. A highly significant difference was observed in the fingerprint ridge density between genders. Conclusions: We conclude that the Barr body in pulpal tissue can be considered as the best possible technique for gender determination within the dental tissues. Lip prints did not show any differences in genders and had no role to play in gender determination. Fingerprint ridge density can also be used to determine gender.

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“…A recent study by Bhardwaj N et al ,[ 22 ] stated that Barr bodies were better analysed and appreciated in histopathological sections than in cytopathological technique.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of cheiloscopy, pulpal tissue and fingerprint for gender identification

Nayak,

Solanki

et al. 2023

Journal of Oral and Maxillofacial Pathology

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Forensic medical cytological examination of the trace evidence on the knife’s blade in the case of multiple penetrating stab/cut wounds in the chest with lung damage: a case report

Kidralieva,

Fedorovtsev,

Kidraliev

2023

Russian Journal of Forensic Medicine

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Background. Various trace evidence are often found on the instruments of mechanical injury, and among them are microparticles of humans damaged organs and tissues. The detection of cells and microparticles of organs and tissues on the instrument of injury confirms the fact of its contact with the human body, which is of great evidentiary value. Case presentation. The article presents the results of forensic medical cytological examination of biological evidence taken from the knifes blade in the case of multiple penetrating stab/cut wounds of the chest with а lung damage. Microscopic preparations were prepared from the traces on knifes blade, which were stained with solutions of fluorochromes (0.0005 % aqueous solution of atebrine and 0.01 % solution of acridine orange). The study was performed using the luminescent microscope "LEICA DM 2500" (объективы 20х и 60х, окуляр 10х). Within the research the microparticles of striated skeletal muscle, areolar connective tissue and fat tissue with fragments of capillaries and arterioles were detected. The isolated mesothelial cells and type II pneumonocytes were also detected. The fibers of striated skeletal muscle tissue looked like long multinucleated cells with distinguishing cross-striation. Areolar connective tissue was represented by loosely arranged fibers which were embedded in the ground substance. The fragments of fat tissue looked like the "clusters" of round and oval large cells, tightly pressed together, with capillaries and arterioles between. Y-chromatin was detected in the nuclei of most cells. In addition, human blood was found on the knifes blade. Conclusion. Thus, as a result of the study, tiny particles of tissues and isolated human cells of the male genetic sex were detected, their organ-tissue affiliation was established, which is a valuable diagnostic find, since it confirms the fact of contact of the trauma instrument with the human body.

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Issues on forensic diagnostics for pyrethroid lambda-cyhalothrin poisoning

Chizhikova¹,

Gornostaev²,

Shigeev³

2023

Russian Journal of Forensic Medicine

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The article presents a case of poisoning with lambda-cyhalothrin, a synthetic pyrethroid, in a family of three (2 deaths at different times of the course of poisoning). A case from our practice is of interest not only from the point of view of the frequency of occurrence of this type of poisoning, but also as an example of a complex diagnostic process, including an analysis of the circumstances, clinical data and a set of post-mortem studies, taking into account the detection of a toxicant that has the ability to break down in minimal quantities in the food samples from the scene. Data indicating polymorphism of manifestations of poisoning with synthetic pyrethroids is given. A post-mortem study revealed biochemical and micromorphological markers of this poisoning diagnosed with acute toxic hepatitis, lesions of the gastrointestinal tract, resulted in hepatic and renal failure with laboratory confirmation, excretory and necrotic nephrosis with severe hemocirculatory disorders in internal organs and tissues. Additionally, an experimental model was created on laboratory animals (rats) to confirm the possibility of developing these lesions during the course of poisoning with a synthetic pyrethroid, while in the experimental model not only a similar clinical picture was obtained, but also the same results of laboratory studies. The presented data of foreign studies, a brief epidemiological report, as well as a detailed description of the comprehensive post-mortem diagnosis based on clinical and morphological, toxicology, biochemical, microscopic and experimental data show that in the study of such cases, an individual approach is required for the most accurate answers to the questions and prevention of these poisonings.

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Determination of gender from dental pulp by identification of Barr bodies

Cited by 4 publications

References 16 publications

Comparative analysis of cheiloscopy, pulpal tissue and fingerprint for gender identification

Comparative analysis of cheiloscopy, pulpal tissue and fingerprint for gender identification

Forensic medical cytological examination of the trace evidence on the knife’s blade in the case of multiple penetrating stab/cut wounds in the chest with lung damage: a case report

Issues on forensic diagnostics for pyrethroid lambda-cyhalothrin poisoning

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