1992
DOI: 10.1139/z92-292
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Determination of gender in cetaceans by the polymerase chain reaction

Abstract: PALSBOLL, P. J., VADER, A., BAKKE, I., and RAAFAT EL-GEWELY, M. 1992. Determination of gender in cetaceans by the polymerase chain reaction. Can. J. Zool. 70: 2 166 -2170. We determined the gender of a variety of cetacean species, including both ondotocetes and mysticetes, using the polymerase chain reaction for amplification of the sex chromosome specific regions ZFYIZFX and SRY. This quick and simple method requires extremely small amounts of tissue, and therefore allows gender to be determined from skin bio… Show more

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Cited by 146 publications
(110 citation statements)
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“…Molecular sex determination was carried out by polymerase chain reaction amplification followed by TaqI digestion of the ZFX/ZFY region of the sex chromosomes [26,27]. The minimum travel distance between these locations, via a great circle route rounding Cape Agulhas and Cap Ste Marie, is greater than 9800 km.…”
Section: Methodsmentioning
confidence: 99%
“…Molecular sex determination was carried out by polymerase chain reaction amplification followed by TaqI digestion of the ZFX/ZFY region of the sex chromosomes [26,27]. The minimum travel distance between these locations, via a great circle route rounding Cape Agulhas and Cap Ste Marie, is greater than 9800 km.…”
Section: Methodsmentioning
confidence: 99%
“…All biopsy samples in the present study were determined to be from males through genetic sexing [22][23][24]. In the Gulf of Maine, sampling intentionally targeted males that were at least 10 y old and therefore considered adults [25].…”
Section: Biopsy Samplesmentioning
confidence: 99%
“…There is now considerable evidence that Sry (sex-determining region Y chromosome) is the testis-determining gene (Gubbay et al 1990;Sinclair et al 1990). With rare exception (see, e.g., , Sry is male-specific in all mammal species examined thus far, a sample that includes representatives from three metatherian and seven eutherian orders (Sinclair et al 1990;Foster et al 1992;Pallsboll et al 1992;. It is expressed in the genital ridge at a time consistent with its having a role in testis determination (Gubbay et al 1990;Koopman et al 1991b), and a 14.5-kb mouse genomic DNA fragment containing Sry can induce testis development when injected into (chromosomally female) XX mouse embryos soon after fertilization (Koopman et al 1991a).…”
Section: Introductionmentioning
confidence: 99%