Determination of Genetic Diversity in European Cranberrybush(Viburnum opulus L.) Genotypes Based on Morphological, Phytochemical and ISSR Markers

Abstract

Turkey's plant diversity varies considerably. Many of these plants are native and commercially grown. European Cranberrybush, which is among the fruit species grown economically in the country, is also of interest in terms of health. In this study, it was aimed to determine genetic diversity with morphological, molecular, and phytochemical markers in 24 different genotypes from Kayseri province, which has an important place in the production of European Cranberrybush in Turkey. The results show that wide variations were detected between genotypes in the morphological parameters. While the genotype G13 was the prominent genotype compared to other genotypes in leaf length (130.69 mm), leaf width (135.76 mm) and fruit length (10.01 mm), the range in fruit weights of genotypes varied between 0.16 g and 0.80 g. In ISSR marker analysis, a total of 73 scoreable bands were obtained from 11 different primers, and 44 of these bands were polymorphic bands. The average polymorphism rate in the study was 60.27%, and the similarity index of the genotypes varied between 0.77 and 0.95. Total flavonoid, total phenolic and total anthocyanin contents ranged from 106.28 mg CAE/100 g to 318.87 mg CAE/100 g, 451.23 mg GAE/100 g to 679.57 mg GAE/100 g, 21.36 mg cyn-3-gluc /100 g to 16.48 mg cyn-3-gluc /100 g, respectively. It is thought that the results of the study may be useful to plant breeders in terms of its development and preservation, as well as giving an opinion to the researchers in new studies to be carried out in the European Cranberry.