Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR

Abstract

This research aims to determine genetic diversity of four strains guppy, respectively are japan blue double sword (JBD), japan blue tiger double sword (JBTD), blue moscow (BM), and panda guppy (PG) with RAPD-PCR method. The obtained genetic diversity data is used as guide reference for hybridization between four strains. The research was conducted in September 2020 to April 2021 with explorative methods and in qualitative and quantitative descriptive analysis. The research was carried out in biotechnology Laboratory, Fishery and Marine Sciences Faculty and Central Laboratory,Padjadjaran University, Indonesia.Strains of JBD, JBTD, BM obtained fromCilengkrangSubdistrict, Bandung and PG strain obtained from Parung market, Bogor. Primary OPA-03 (AGTCAGCCAC) is used for standard parameters to interpret genetic diversity among four strains of guppy. Based on results, amplification with OPA-03 primary visualize 25 bands that include five polymorphic bands and 20 monomorphic bands. The phylogenetic tree result show that there are two relationship groups. The first group are JBD, JBTD, and BM with similarity index in the range of 80-89%The first group consist two sub groups of relationship. The first sub group are JBD and JBTD with similarity index of 89%. The second sub group is BM with similarity index of 80%. The second group isPG with similarity index of 65.5%.

KERAGAMAN GENETIK GUPPY (Poecilia reticulata) MENGGUNAKAN METODE RAPD (Random Amplified Polymorphic DNA)

“…Teknik Random Amplified Polymorphic DNA (RAPD) merupakan teknik yang umum digunakan untuk mengetahui polimorfisme DNA (Utomo et al, 2020). RAPD dapat digunakan untuk menganalisis variasi genetik (Hafidah et al, 2021;Neekhra et al, 2014). Polimorfisme DNA dapat berfungsi sebagai penanda molekuler untuk membedakan antar populasi ikan (Utomo et al, 2020).…”

“…Polimorfisme DNA dapat berfungsi sebagai penanda molekuler untuk membedakan antar populasi ikan (Utomo et al, 2020). Teknik ini juga digunakan untuk menentukan keragaman genetik pada ikan Rainbow Kurumoi (Hayuningtyas & Kadarini, 2016) dan empat strain Guppy yaitu Japan Blue Double Sword (JBD), Japan Blue Tiger Double Sword (JBTD), Blue Moscow (BM), dan Panda Guppy (PG) (Hafidah et al, 2021). Keunggulan teknik RAPD yaitu 1) dapat digunakan untuk mendeteksi sekuen nukleotida hanya dengan satu primer, 2) menghasilkan polimorfisme yang tinggi, dan 3) dapat digunakan tanpa mengetahui asal genome sebelumnya (Hafidah et al, 2021).…”

Analysis of Genetic Diversity of Mobula sp. in Cilacap Using RAPD (Random Amplified Polymorphic DNA) Markers

“…According to [35], the base length (bp) found in RAPD DNA fragments in fish ranges from 200 -1500 bp, whereas according to [26], the base length in fish ranges from 300-1500 bp.…”