Determination of Genetic Diversity of Vitis vinifera cv. Kabarcik Populations from the Coruh Valley Using SSR Markers

Ağar, Güleray; Yıldırım, Nalan; Erċışlı, Sezai; Ergül, Ali; Yüksel, Cabir

doi:10.1007/s10528-011-9492-y

Cited by 10 publications

(4 citation statements)

References 38 publications

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“…As shown in table 2, VMC5G1 locus showed the lowest number of different alleles (Na) (three alleles) and VMC1C10 locus observed the highest (nine alleles) with a total of 63 alleles considering all loci (in an average of 5.25 alleles). Previous studies on grapes confirmed that the VVS2 locus was given the highest number of alleles (Nunez et al, 2004;Şelli et al, 2007;Tangolar et al, 2009;Agar et al, 2012). The average number of different alleles in the present study was 5.25, which is relatively similar to the average number of alleles observed by previous studies (Emanuelli et al, 2013).…”

Section: Resultssupporting

confidence: 90%

Molecular characterization of autochthonous grapevine (Vitis vinifera L.) cultivars in Kurdistan using simple sequence repeats (SSRs)

Mahmood

Ali

Hawezy

2019

گۆڤاری زانستە مرۆڤایەتیەکانی زانکۆی گەرمیان

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Grapevine (Vitis vinifera L.) is one of the oldest and the most extensively grown fruit plants worldwide with regards to its great economic significance throughout the world. Northern part of Iraq (Kurdistan) is regarded as one of the regions in Iraq where grapevine plants have been taken into cultivation. Despite the economic importance of grapevine cultivars in this region, they have not been precisely classified and their genetic data is also limited. In the present study 12 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity among 13 local grape cultivars, also determining their genetic relationships with four Eurpean ones (17 cultivars studied). Results showed that the observed number of alleles (N) per locus varied from 3 to 9 with an average of 5.25 and the values of polymorphism information content (PIC) ranged from 0.28 to 0.776. Despite the average value (0.1716) of observed heterozygosity (Ho) was less than the average level (0.6427) of expected heterozygosity (He), results were relatively demon strated a random union of gametes in the population. In addition, genetic similarity among respective cultivars ranged from 0.17 to 0.1, indicating valuable genetic variation among studied cultivars. The analysis of UPGMA clustering demonstrated high genetic variation among those cultivars and grouped them into three main clusters. Findings from the present study might render striking information in determining local cultivars and in breeding management strategies that are of importance for genetic conservation and cultivar improvement.

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Section: Resultssupporting

confidence: 90%

Molecular characterization of autochthonous grapevine (Vitis vinifera L.) cultivars in Kurdistan using simple sequence repeats (SSRs)

Mahmood

Ali

Hawezy

2019

گۆڤاری زانستە مرۆڤایەتیەکانی زانکۆی گەرمیان

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“…Moreover, in a set of six SSR markers from four cultivated populations of V. vinifera cv. containing 60 individuals, expected heterozygosity varied from 0.126 (VVMD7) to 0.790 (VVS2), with the overall average of 0.574 (Agar et al, 2012). da Silva et al (2015) reported that the numbers and frequencies of alleles in genetic diversity studies affect the marker heterozygosity.…”

Section: Nuclear Microsatellites Polymorphism Among the Grapevine Accessionsmentioning

confidence: 99%

“…Based on their PIC, the most polymorphic loci were VVSMD25 (0.87) followed by VVS1 (0.85) and ISV3 (0.81), whereas VVS3 (0.50), ISV3 (0.50) and VVSMD6 (0.55) showed the lowest PIC (Table 3). Several studies on Vitis genus indicated that the most informative SSR markers included VVS2, VVMD5, VVS5, VVMD14, VVMD28, and VVMD36 (Agar et al, 2012;Borrego et al, 2001;Crespan and Milani, 2001;Ibañez et al, 2003;Lefort and Roubelakis-Angelakis, 2001).…”

Section: Nuclear Microsatellites Polymorphism Among the Grapevine Accessionsmentioning

confidence: 99%

Microsatellite Markers Analysis for the Genetic Characterization and Relationships among Some of Iranian Local Grapevine Accessions (Vitis ViniferaL.)

Taheri

Ramandi

2020

International Journal of Fruit Science

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One of the most important requirements for cultivar identification in Vitis species is rapid and consistent genotyping using DNA-based markers. Genetic characterization and diversity of grapevine cultivars using molecular markers have been the subject of many studies. The Iranian grapevine biodiversity is one of the most important in the Middle East. In the present work, fourteen nuclear microsatellite markers were used to assess the genetic diversity and relationships among 25 local grapevine accessions collected from vine-growing areas of Qazvin province (Iran). A total of 81 distinct alleles were produced with amplified product sizes ranging from 91 to 432 bp. The number of observed alleles for each locus ranged from 2 (ISV3) to 9 (VVS3 and VVMD6), with the overall average of 5.79 allele per locus. The observed heterozygosity (Ho) ranged from 0.53 (ISV3) to 0.72 (VVSMD25), while the expected heterozygosity (He) varied between 0.49 (ISV3) and 0.87 (VVSMD25). The polymorphism information content (PIC) ranged between 0.50 for VVS3 and ISV3 to 0.87 for VVSMD25. However, Shannon's information index (I) was found to be highest (2.13) in VVSMD25 locus, while the VVS3 locus had the lowest value with an average of 0.75 among SSR markers. According to Jaccard's similarity coefficient and UPGMA algorithm, cluster analysis assigned the accessions into five groups. Despite similar names, the results revealed that the accessions belonged to different groups. The obtained PCoA scatter plots further strongly supported their UPGMA dendrogram results. Beside VVMD, VVS series of markers were also used in the present study and the results showed that these series of microsatellite markers were as efficient as VVMD series in terms of discrimination and polymorphism for studying grapevine cultivars. Among all the microsatellite loci, VVSMD25 (PID = 0.03, PIC = 0.87) was found to be the most informative among the accessions.

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“…Most previous studies have focused on V. vinifera L. cultivars from a single location (Agar et al, 2012), which limits the utilization of the species to some extent. In this work, we selected 49 grape germplasm accessions originating from several different countries and investigated their genetic diversity and evolutionary relationships using 19 SSR markers.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of grape germplasm as revealed by microsatellite (SSR) markers

Wang¹,

Zhang²,

Liu³

et al. 2015

Afr. J. Biotechnol.

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In this work, cluster analysis and principal component analysis (PCA) were used to study the genetic diversity and relationships among 49 grape germplasm accessions analyzed with 19 simple sequence repeat (SSR) primer pairs. In total, 139 polymorphic loci were detected among these accessions with an average of 7.32 polymorphic loci per SSR primer pair. The average values for the effective number of alleles, Nei's gene diversity, and Shannon's information index were 1.5605, 0.3352 and 0.5064, respectively. The cluster analysis showed that the 49 accessions could be divided into five groups and an outgroup. The results of the PCA were nearly consistent with those of unweighted pair-group method with arithmetic averages (UPGMA) clustering analysis. These results will be useful for the exploitation of grape germplasm in basic and applied research.

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Determination of Genetic Diversity of Vitis vinifera cv. Kabarcik Populations from the Coruh Valley Using SSR Markers

Cited by 10 publications

References 38 publications

Molecular characterization of autochthonous grapevine (Vitis vinifera L.) cultivars in Kurdistan using simple sequence repeats (SSRs)

Molecular characterization of autochthonous grapevine (Vitis vinifera L.) cultivars in Kurdistan using simple sequence repeats (SSRs)

Microsatellite Markers Analysis for the Genetic Characterization and Relationships among Some of Iranian Local Grapevine Accessions (Vitis ViniferaL.)

Genetic diversity of grape germplasm as revealed by microsatellite (SSR) markers

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