Determination of genotoxic effects of methidathion alkaline hydrolysis in human lymphocytes using the micronucleus assay and square-wave voltammetry

Stivaktakis, Polychronis; Giannakopoulos, Evangelos; Vlastos, Dimitris; Matthopoulos, Demetrios P.

doi:10.1016/j.bioelechem.2016.08.004

Cited by 5 publications

(4 citation statements)

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“…It is well established that MN assay is a sensitive indicator of genomic damages of exogenous and endogenous origin (23,27). MNf in PBLs represents an indirect, intracellular indicator of chromosomal and genomic instability (high levels of MN are indicative of extended damages of the DNA repair system and in chromosomal division) (17,(28)(29)(30).…”

Section: Discussionmentioning

confidence: 99%

Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer

et al. 2019

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Colorectal cancer (CRC) is the third most diagnosed type of cancer affecting males, and the second most diagnosed type of cancer affecting females, and one of the leading causes of cancer-related mortality globally. The estimation of the micronuclei (MN) frequency in peripheral blood lymphocytes (PBLs) from patients with CRC is proposed as a prognostic/predictive easy-to-use biomarker. In this study, we aimed to investigate the effects of systemic treatment on the MN frequency in PBLs from patients with CRC in order to determine the effectiveness of the MN frequency as a biomarker. For this purpose, from 2016 to 2018, we quantified the MN frequency as a prognostic/predictive biomarker in serial samples from 25 patients with metastatic CRC (mCRC) using cytokinesis block micronucleus assay (CBMN assay). The MN frequency in the PBLs of the patients was evaluated before, during the middle and at the end of the therapy (approximately 0, 3 and 6 months). The results revealed a common pattern regarding the fluctuation in the MN frequency. Statistical analysis confirmed that when the disease response was estimated with radiological criteria, a good response was depicted at the MN frequency and vice versa. Consequently, the findings of this study suggest that the MN frequency may serve as a promising prognostic/predictive biomarker for the monitoring of the treatment response of patients with CRC.

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Section: Discussionmentioning

confidence: 99%

Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer

et al. 2019

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“…These findings are important because early identification of those patients who are more likely to develop progressive disease can allow clinicians to take early decisions on therapy selection. A possible explanation of our findings lies in the fact that MN assay is a sensitive indicator of genomic damages of exogenous and endogenous origin ( 31 , 32 ), while MNf in PBLs, despite the unknown underlying mechanism, is shown to positively correlate with chromosomal and genomic instability ( 5 ), which is one of the pillars in colorectal carcinogenesis. Therefore, the emergence of a resistant cluster of cancer cells against the sensitive “background” can be indirectly identified through MNf.…”

Section: Discussionmentioning

confidence: 83%

Novel Prognostic Biomarkers in Metastatic and Locally Advanced Colorectal Cancer: Micronuclei Frequency and Telomerase Activity in Peripheral Blood Lymphocytes

et al. 2021

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PurposeDue to the current practice on colorectal cancer (CRC) management, chemoresistance is most often recognized at the end of the treatment. Therefore, effective and easy-to-use prognostic biomarkers are needed.Experimental DesignWe evaluated the prognostic significance of two novel CRC biomarkers: a) micronuclei frequency (MNf) in 55 metastatic CRC (mCRC) and 21 locally advanced rectal cancer (laRC) patients using cytokinesis block micronucleus assay (CBMN assay) and b) telomerase activity (TA) in 23 mCRC and five laRC patients using TRAP-ELISA. Both biomarkers were evaluated in peripheral blood lymphocytes (PBLs) before, at the middle, and at the end of the therapy (approximately 0, 3, and 6 months) for mCRC patients before, at the end of the therapy, and after surgery for laRC patients.ResultsOverall, MNf demonstrated significant prognostic value since a decrease of MNf less than 29% between middle and initial MNf measurements can discriminate between progressive and stable/responsive disease with sensitivity of 36% and specificity of 87.0% while being able to identify responsive disease with sensitivity of 72.7% and specificity of 59.3%. On the other hand, TA presented a significant trend of increase (p = 0.07) in patients with progressive disease at the middle measurement.ConclusionsThe findings of this study suggest that the MN frequency may serve as a promising prognostic biomarker for the monitoring of the treatment response of patients with CRC, while TA should be evaluated in a larger group of patients to further validate its significance.

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“…In particular, it was shown that pesticides, especially organochlorines and organophosphates, inhibit hemopoiesis [[2], [3], [4]], whereas in cases of synthetic pyrethroid and neonicotinoid exposure, an increase of oxidative stress marker levels and telomerase activity were found with additional increased inflammations of various organs, and inflicted genotoxicity [[5], [6], [7], [8]]. Furthermore, other pesticide groups, regarding the organophosphorus and carbamate type, have been also studied for their histopathological lesions, oxidative stress and genotoxic effects [9,10]. Biomonitoring of such pesticides has gained attention throughout the years as seen in studies focusing on neonicotinoids and organophosphorus pesticides [11,12] and ways of ascertaining information based on a “real-life human exposure” from on a long-term and low-dose exposure to a specific chemical mixture when not simulated as a real-life condition of exposure, are not clear, leading to “grey zones” in the interpretation of the results due to uprising limitations.…”

Section: Introductionmentioning

confidence: 99%

Maximum tolerated doses and erythropoiesis effects in the mouse bone marrow by 79 pesticides’ technical materials assessed with the micronucleus assay

et al. 2019

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Determination of genotoxic effects of methidathion alkaline hydrolysis in human lymphocytes using the micronucleus assay and square-wave voltammetry

Cited by 5 publications

References 55 publications

Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer

Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer

Novel Prognostic Biomarkers in Metastatic and Locally Advanced Colorectal Cancer: Micronuclei Frequency and Telomerase Activity in Peripheral Blood Lymphocytes

Maximum tolerated doses and erythropoiesis effects in the mouse bone marrow by 79 pesticides’ technical materials assessed with the micronucleus assay

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