Determination of gentamicin in different matrices by a new sensitive high-performance liquid chromatography-mass spectrometric method

Lecároz, Concepción; Campanero, Miguel Ángel; Gamazo, Carlos; Blanco-Prieto, María J.

doi:10.1093/jac/dkl258

Cited by 50 publications

(30 citation statements)

References 12 publications

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“…The results here showed that there were no significant changes in the pH of the formulations. All the formulations were considered stable based on the fact that the pH of the gentamicin-lipospheres formulations were within the value given for a conventional marketed gentamicin (pH [6][7][8] which is in accordance to the literature [23] . The slight change in the pH is neither from the lipids nor the drug, because a similar change was also observed in the unloaded lipospheres (no drug).…”

Section: 1surface Morphologymentioning

confidence: 69%

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Phospholipon 90H (P90H)–based PEGylated microscopic lipospheres delivery system for gentamicin: an antibiotic evaluation

Momoh

Esimone

2012

Asian Pacific Journal of Tropical Biomedicine

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Section: 1surface Morphologymentioning

confidence: 69%

“…Hence, the in vitro release of gentamicin from the formulation was studied. In vitro release was evaluated using a dialysis bag diffusion technique as described by previous researcher [23,24] . The dialysis bags were hydrated in phosphate-buffered saline, pH 7.2 overnight before the experiment.…”

Section: In Vitro Drug Release Studymentioning

confidence: 99%

Phospholipon 90H (P90H)–based PEGylated microscopic lipospheres delivery system for gentamicin: an antibiotic evaluation

Momoh

Esimone

2012

Asian Pacific Journal of Tropical Biomedicine

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“…The MIC and MBC of GEN for Brucella were 0.25 and 0.5 g/ml, respectively (14). The time during which the antibiotic concentrations exceeded the MBC in the spleen was similar over time for the two selected formulations, but GEN remained in the liver longer when it was administered in 502H microspheres (14 day) than in the 75:25H formulation (6.1 days).…”

Section: Resultsmentioning

confidence: 94%

“…Briefly, a known amount of the loaded particles was dispersed in 1 ml of 0.1 N NaOH, the samples were centrifuged (25,000 ϫ g, 15 min), and the GEN in the supernatants was quantified by HPLC-mass spectrometry (14).…”

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confidence: 99%

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Poly( d , l -Lactide-Coglycolide) Particles Containing Gentamicin: Pharmacokinetics and Pharmacodynamics in Brucella melitensis - Infected Mice

Lecaroz

Blanco-Prieto

Campanero

et al. 2007

Antimicrob Agents Chemother

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Drug delivery systems containing gentamicin were studied as a treatment against experimental brucellosis in mice. Micro-and nanoparticles prepared by using poly(D,L-lactide-coglycolide) (PLGA) 502H and microparticles made of PLGA 75:25H were successfully delivered to the liver and the spleen, the target organs for Brucella melitensis. Both polymers have the same molecular weight but have different lactic acid/glycolic acid ratios. Microparticles of PLGA 502H and 75:25H released their contents in a sustained manner, in contrast to PLGA 502H nanoparticles, which were degraded almost completely during the first week postadministration. The values of the pharmacokinetic parameters after administration of a single intravenous dose of 1.5 mg/kg of body weight of loaded gentamicin revealed higher areas under the curve (AUCs) for the liver and the spleen and increased mean retention times (MRTs) compared to those for the free drug, indicating the successful uptake by phagocytic cells in both organs and the controlled release of the antibiotic. Both gentamicin-loaded PLGA 502H and 75:25H microparticles presented similar pharmacokinetic parameter values for the liver, but those made of PLGA 75:25 H were more effective in targeting the antibiotic to the spleen (higher AUCs and MRTs). The administration of three doses of 1.5 mg/kg significantly reduced the load associated with the splenic B. melitensis infection. Thus, the formulation made with the 75:25H polymer was more effective than that made with 502H microspheres (1.45-log and 0.45-log reductions, respectively, at 3 weeks posttreatment). Therefore, both, pharmacokinetic and pharmacodynamic parameters showed the suitability of 75:25H microspheres to reduce the infection of experimentally infected mice with B. melitensis.Brucellosis remains a major zoonosis worldwide (17), and it is among the microorganisms most likely to be used as biological weapons (24). Specific therapy reduces morbidity, shortens the duration of illness, and decreases the incidence of complications (31); however, relapse currently presents a significant problem (27). Long-lasting combined drug treatments are difficult to achieve (27); and also the intracellular location of the pathogen in phagocytic cells, mainly in the liver and the spleen, are involved in therapeutic failure (22). Drug delivery systems containing gentamicin (GEN) have been developed as potential alternatives to classical therapy, and the usefulness of these vectors has been shown (5, 7, 10, 23, 30). Although GEN is effective against the pathogen in vitro (11, 18, 25), it shows a low level of intracellular penetration due to its polar nature; hence, its entrapment in particulate carriers might facilitate the entrance of the drug into the cells. Moreover, sustained drug delivery could avoid long-term treatment, eliminating the toxic systemic levels achieved during classical antibiotic therapy with the free drug. Despite the promising results obtained with GEN-containing liposomes (7,10,30), important drawbacks are attributed to the...

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Applying Charged Aerosol Detection to Aminoglycosides

Joseph

Rustum

2017

Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques

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Determination of gentamicin in different matrices by a new sensitive high-performance liquid chromatography-mass spectrometric method

Abstract: All these data favour the use of chromatography-mass spectrometry as a versatile technique not only suitable for gentamicin quantification loaded in drug delivery systems, but also sensitive and specific enough for in vivo and intracellular studies.

Cited by 50 publications

References 12 publications

Phospholipon 90H (P90H)–based PEGylated microscopic lipospheres delivery system for gentamicin: an antibiotic evaluation

Phospholipon 90H (P90H)–based PEGylated microscopic lipospheres delivery system for gentamicin: an antibiotic evaluation

Poly( d , l -Lactide-Coglycolide) Particles Containing Gentamicin: Pharmacokinetics and Pharmacodynamics in Brucella melitensis - Infected Mice

Applying Charged Aerosol Detection to Aminoglycosides

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