Determination of Glimepiride in Rat Serum by Rp-Hplc Method

Glimepiride (GMD) is a third-generation sulfonylurea derivative and one of the top three most-prescribed oral antidiabetic drugs. The need for a depot formulation exists, and a safe and effective antidiabetic therapy is the goal of this study. The aims were to design a depot in situ gel (ISG) formulation and investigate the main factors that control the initial burst and sustain the GMD effect using the Box-Behnken design. The studied factors were polymer percent (X1), plasticizer percent (X2) and benzyl benzoate percent in N-methyl-2-pyrrolidone (X3). The results revealed that X2 is the only factor that showed significant effects on all investigated responses. Scanning electron microscopy images showed that an increase in PEG % improved the smoothness and reduced the porosity of the ISG formulation surface. The GMD plasma levels in diabetic rats revealed no significant difference (p < 0.05) between the maximum GMD plasma concentrations of the optimized GMD-ISG formula (10 mg/ kg) and oral marketed GMD tablets (1 mg/kg). This result ensures that the optimized formula does not exceed the maximum safe plasma concentration. In addition, the optimized GMD-ISG formulation showed a depot effect that lasted for 14 days post-injection. This approach to controlling GMD release using an in situ forming system could be useful for improving patient compliance and diabetes treatment effectiveness.

show abstract

“…Plasma GMD concentrations were analysed after liquid-liquid extraction with methanol using the Sujatha et al . method 25 .…”

Section: Methodsmentioning

confidence: 99%

A PLGA-reinforced PEG in situ gel formulation for improved sustainability of hypoglycaemic activity of glimepiride in streptozotocin-induced diabetic rats

Ahmed

El-Say

Alahdal

2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…AUC total was calculated using mixed log linear model. The pharmacokinetic data is presented in Table 5 [27][28][29] .…”

Section: Bmentioning

confidence: 99%

Development and Validation of a HPLC-based Bioanalytical Method for Lorcaserin using Solid Phase Extraction and Application to a Pharmacokinetic Study in Rats

Rajput¹,

Sathe²,

Patel³

2018

pharmaceutical-sciences

View full text Add to dashboard Cite

“…Several methods were applied for the determination of glimepiride separately or in combination with other drugs in pharmaceutical formulations and biological samples, such as spectrophotometry,[17] ultraviolet spectrophotometric,[18] reversed-phase high-performance liquid chromatography (RP-HPLC),[19] liquid chromatography–mass spectrometry (LC-MS/MS),[1220] liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS),[21] and recently high-performance liquid chromatography–mass spectrometry (HPLC-MS). [14]…”

Section: Introductionmentioning

confidence: 99%

Development and validation of liquid chromatography method for determination of glimepiride in presence of (Vimto®) soft drinks in rats: application to pharmacokinetics studies

Hamad¹,

Rahhal²,

Dayyih³

et al. 2019

J Pharm Bioall Sci

View full text Add to dashboard Cite

Context: Diet and beverages are thought to have notable effects on drugs. Recently, this relationship has received significant consideration. Aims: To develop and validate a simple, rapid, and sensitive method for the determination of glimepiride in rat serum. This will be performed using high-performance liquid chromatography–mass spectrometry (HPLC-MS/MS). Potential pharmacokinetic interactions between glimepiride and the soft drink, Vimto, will also be investigated in the serum of experimental rats. Materials and Methods: HPLC-MS/MS was constructed and clarithromycin was used as an internal standard. Results: The method was validated in terms of linearity, precision, accuracy, stability, and system suitability parameters. The method was found to be satisfactory and suitable for the determination of glimepiride. The precision of glimepiride was high (coefficient of variation, CV% <15%), the accuracy over all 3 days of validation was within the accepted criteria. Glimepiride peak serum concentration ( C max ) was 126.01 ng/mL and was reached within 1 h ( T max ) of administration. Mean area under curve (AUC) was 964.70 ng/mL and was reached within 24 h of administration. The Vimto soft drink significantly ( P < 0.050) reduced glimepiride peak serum concentration to 57.87 ng/mL and was reached within 2 h of administration. AUC was significantly reduced to 335.04 ng * h/mL ( P < 0.050). Conclusion: Glimepiride pharmacokinetic parameters such as C max and AUC were significantly affected by the Vimto soft drink. Therefore, this study developed a simple, rapid, and sensitive method for validation and determination of the effects of soft drinks on drugs using the LC-MS/MS method.

show abstract

Determination of Glimepiride in Rat Serum by Rp-Hplc Method

Cited by 19 publications

References 16 publications

A PLGA-reinforced PEG in situ gel formulation for improved sustainability of hypoglycaemic activity of glimepiride in streptozotocin-induced diabetic rats

A PLGA-reinforced PEG in situ gel formulation for improved sustainability of hypoglycaemic activity of glimepiride in streptozotocin-induced diabetic rats

Development and Validation of a HPLC-based Bioanalytical Method for Lorcaserin using Solid Phase Extraction and Application to a Pharmacokinetic Study in Rats

Development and validation of liquid chromatography method for determination of glimepiride in presence of (Vimto®) soft drinks in rats: application to pharmacokinetics studies

Contact Info

Product

Resources

About