Determination of Glucose and Cholesterol Using a Novel Optimized Luminol- CuO Nanoparticles-H2O2 Chemiluminescence Method by Box–Behnken Design

Abstract

In this study, a simple, rapid and sensitive luminol-CuO nanoparticles-H2O2 chemiluminescence (CL) method has been proposed for determination of glucose and cholesterol in plasma. Response surface methodology (RSM) based on Box-Behnken design (BBD) was used to optimize the most important operating variables (solution pH effect and the CL reagents concentration) of luminol CL system. In optimum conditions, it was found that CuO nanoparticles (NPs) could enhance the CL intensity and the method sensitivity towards evaluation of trace amount of glucose and cholesterol. Under the optimal conditions, there is a good linear relationship between the luminol-CuO NPs - H2O2 relative CL intensity and the concentration over the range of 1.2 × 10(-6)-1.0 × 10(-3) M (R (2) = 0.9991) for glucose and 2.5 × 10(-5)-CuO NPs7.17 × 10(-3) M (R (2) = 0.9968) for cholesterol and with a 3σ detection limit of 7.1 × 10(-7) and 6.4 × 10(-6) M, respectively. The relative standard deviations (RSD) < 3.3% were obtained.

Au NCs-enhanced chemiluminescence from NaHSO3–H2O2 and its analytical application

“…It was reported that H 2 O 2 decomposition on supported metal catalysts such as Au NPs, Ag NPs and CuO NPs involved the formation of hydroxyl radicals OHc. [40][41][42] In the same manner, we suggested that the O-O bond of H 2 O 2 might be broken up into double OHc radicals by virtue of the catalysis of Au nanoclusters. The OHc reacted with cSO 3 À to form HSO 4…”

Enhanced luminol–O2 chemiluminescence reaction by CuO nanoparticles as oxidase mimics and its application for determination of ceftazidime

“…25,26 The application realm of the system was then further expanded by other researchers. [27][28][29][30][31][32] However, to the best of our knowledge, the oxidase-like activity of CuO NPs, a discovered 33 but less known property of CuO NPs, has somehow been neglected and has not yet been incorporated in analytical applications. It should be noted that the oxidase-like activity of V 2 O 5 nanowires 34 and CoFe 2 O 4 NPs 35 toward the luminol-O 2 CL reaction has also been studied.…”

Hydrogen Peroxide Sensors for Biomedical Applications

“…In 2019, Wang et al decreased the H 2 O 2 detection limit of the luminol reaction via the synthesis and use of a hemin and poly(ethylene glycol) methyl ether catalyst [36]. The new catalyst resulted in a detection limit of 1.8 nM [36], lower than other luminol-H 2 O 2 detection levels [31,36,[43][44][45].…”