Determination of Glufosinate Ammonium and Its Metabolite (AE F064619 and AE F061517) Residues in Water by Gas Chromatography with Tandem Mass Spectrometry after Ion Exchange Cleanup and Derivatization

Abstract

An analytical method for the determination of glufosinate ammonium and its principal metabolites, AE F064619 and AE F061517, in water of two different hardnesses (5 and 30 DH, French hardness) has been developed and validated. Samples were spiked at different levels (0. 05 and 0.5 microgram/L) and were purified by column chromatography on ion-exchange resins. After derivatization with glacial acetic acid and trimethylarthoacetate mixture, the derivatives were quantified by using capillary gas chromatography with an ion-trap tandem mass spectrometric detector. Analytical conditions for MS/MS detection were optimized, and the quantification was carried out on the areas of the most representative ions. The limit of quantification was validated at 0.05 microgram/L for each compound. The mean recovery value and the relative standard deviation (n = 20) were 92.0% and 17. 8% for glufosinate ammonium, 90.2% and 15.8% for AE F064619, and 89. 7% and 12.7% for AE F061517.

A rapid liquid chromatography method for determination of glufosinate residue in maize after derivatisation

“…Glufosinate phosphonic acid] is a broad-spectrum, non-selective and post-emergent herbicide used for the control of a wide range of weeds (Royer, Beguin, Sochor, & Communal, 2000;Zimdahl, 1993). It has been used to control weeds in the farmland such as maize, wheat, soybean and so on (MAFF, 1990).…”

“…Analytical methods for the analysis of glufosinate include capillary electrophoresis (CE) (Chang, Tseng, Mallipattu, & Chang, 2005;Goodwin, Startin, Keely, & Goodall, 2003;Orejuela & Silva, 2005;Zhou et al, 2007), enzyme-linked immunosorbent assay (ELISA) (González-Martínez et al, 2005;Ibáñez, Pozo, Sancho, López, & Hernández, 2005), gas chromatography (GC) (Kataoka, Ryu, Sakiyama, & Makita, 1996;Motojyuku et al, 2008;Royer et al, 2000;Tseng, Lo, Chang, Chou, & Chang, 2004) and liquid chromatography (LC) (Hanke, Singer, & Hollender, 2008;Hori, Fujisawa, Shimada, Sato, Kikuchi, et al, 2002;Kuster, Alda, & Barceló, 2009;Sadi, Vonderheide, & Caruso, 2004;Sancho, Hernandez, López, Hogendoorn, Dijkman, & van Zoonen, 1996;Wang, Chen, Hsu, Cheng, & Lee, 2008). LC is preferred over GC because of the ionic character of glufosinate.…”

HPLC-MS/MS of Highly Polar Compounds

“…A general drawback of the methods using diazomethane is the toxic and explosive nature of the reagent; special care needs to be taken to ensure safety of all operations. Alternative alkylations of aminoalkyl phosphonic acids could be performed with fluorinated alcohols/perfluorinated anhydrides mixtures (Deyrup et al, 1985) or trimethyl orthoacetate (Royer et al, 2000, Kudzin et al (2003. Deyrup et al (1985) analysed glyphosate (N-phosphonomethylglycine) and its major metabolite, aminomethylphosphonic acid, from water samples by GC with flamephotometric, electron capture or mass spectrometric detection, after derivatization with trifluoroethanol (TFE) and trifluoroacetic anhydride (TFAA).…”

“…An LLOQ of 0.5 μg/L in deionized water was obtained with electron capture detection. Trimethylorthoacetate (TMOA) was preferred by Royer et al (2000) for the derivatization of another herbicide, gluphosinate (DL homoalanine-4-yl (methyl)phosphonic acid), and two of its metabolites, 3-methylphosphonico-propionic and 2-methylphosphonico-acetic acids, prior to analysis by GC-MS/MS with chemical ionization. This method was applied for the quantification of gluphosinate and its metabolites in water samples, over a range of 10-150 μg/L.…”

Enantioselective hydrolyzation and photolyzation of dufulin in water

“…The most commonly used chromatographic separation methods include high-performance liquid chromatography (HPLC) [9,10], gas chromatography (GC) [11], liquid chromatography-tandem mass spectrometry (LC/MS) [12], gas chromatography-tandem mass spectrometry (GC-MS/MS) [13,14], capillary electrophoresis (CE) [15-18], and enzyme-linked immunosorbent assay (ELISA) [2,3,19]. For example, an analytical method has been reported for quantifying dl -glufosinate enantiomers in biological specimens using precolumn derivatization and reversed-phase HPLC with a fluorescence detector.…”