Determination of glutathione and glutathione disulfide using liquid chromatography: A review on recent applications

Tsiasioti, Apostolia; Tzanavaras, Paraskevas D.

doi:10.1016/j.microc.2023.109157

Cited by 14 publications

(2 citation statements)

References 85 publications

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“…From the analytical chemistry point of view, a search in the scientific bibliography has revealed a persistent interest in developing new methods for the analytical determination of GSH with many instrumentation techniques including high-performance liquid chromatography [ 5 , 6 , 7 ], zone fluidics [ 8 ], capillary electrophoresis [ 9 ], electroanalysis [ 10 ], resonance light scattering [ 11 ], mass spectrometry [ 12 , 13 ], and chemiluminescence [ 14 , 15 ]. The most popular methods for GSH determination are colorimetric procedures [ 16 , 17 , 18 , 19 , 20 ], fluorescence methods [ 21 , 22 , 23 , 24 ] with the addition of nanostructures, and spectrophotometric or fluorimetric methods after derivatization [ 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

Tarara,

Tzanavaras,

Tsogas

2024

Molecules

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Herein, a new, direct paper-based fluorimetric method is described for the quantitative determination of glutathione (GSH) molecules in nutritional supplements. Briefly, the proposed analytical method is based on the fluorescence emission resulting from the direct and selective chemical reaction of GSH molecules with the derivatization reagent that is o-phthalaldehyde (OPA) in acidic conditions at room temperature. The intensity of the emitted fluorescence on the surface of the analytical paper devices after irradiation with a lamp at 365 nm is proportional to the concentration of GSH and is measured using a smartphone as the detector. This methodology, which is suitable for measurements in laboratories with limited resources, does not require specialized instrumentation or trained personnel. The protocol governing the proposed method is simple and easily applicable. Essentially, the chemical analyst should adjust the value of pH on the surface of the paper by adding a minimal amount of buffer solution; then, after adding a few microliters of the derivatization reagent, wait for the surface of the paper to dry and, finally, add the analyte. Subsequently, the irradiation of the sensor and the measurement of the emitted fluorescence can be recorded with a mobile phone. In the present study, several parameters affecting the chemical reaction and the emitted fluorescence were optimized, the effect of interfering compounds that may be present in dietary supplements was examined, and the stability of these paper sensors under different storage conditions was evaluated. Additionally, the chemical stability of these paper devices in various maintenance conditions was studied, with satisfactory results. The detection limit calculated as 3.3 S/N was 20.5 μmol L−1, while the precision of the method was satisfactory, ranging from 3.1% (intra-day) to 7.3% (inter-day). Finally, the method was successfully applied to three different samples of dietary supplements.

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Section: Introductionmentioning

confidence: 99%

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

Tarara,

Tzanavaras,

Tsogas

2024

Molecules

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“…For the prompt and precise diagnosis and treatment of significant illnesses including diabetes, Alzheimer's disease, and cancer [22,23], it is of utmost importance to establish a dependable and accurate GSH detection method. There are many methods for GSH detection, such as capillary electrophoresis [24], electrochemistry [25], MS surface-enhanced Raman scattering [26], HPLC [27], etc. Although a variety of techniques have emerged in the field of GSH detection, these highly sensitive techniques are often accompanied by long pretreatment times, difficult operation, and high cost, making it impossible to detect quickly clinically [28].…”

Section: Introductionmentioning

confidence: 99%

MXene/Carbon Dots Nanozyme Composites for Glutathione Detection and Tumor Therapy

Lu,

Jia,

Wang

et al. 2024

Nanomaterials

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Co-N-CDs-based MXene nanocomposites (MXene@PDA/Co-N-CDs) were constructed by decorating Co-N-CDs on polydopamine-functionalized MXene nanosheets. Both Co-N-CDs and MXene nanosheets have peroxidase-like activity; when the two materials are combined to form MXene@PDA/Co-N-CDs nanocomposites, the peroxide-like activity can be further enhanced. MXene@PDA/Co-N-CDs could oxidize the substrate 3,3′5,5′-tetramethylbenziline (TMB) to form ox-TMB, as confirmed by detecting the absorption of the blue products. A highly selective colorimetric biosensor was developed for the determination of glutathione (GSH) in the concentration range of 0.3 to 20 µM with a lower detection limit (LOD) of 0.12 µM, which realized the accurate detection of GSH in human serum and urine samples. Moreover, in the tumor microenvironment, MXene@PDA/Co-N-CDs could catalyze hydrogen peroxide to produce hydroxyl free radicals and produce a photothermal effect under the exposure of NIR-I irradiation. The catalytic activity of MXene@PDA/Co-N-CD nanocomposites was fully achieved for the death of cancer cells through photothermal/photodynamic synergistic therapy. The MXene@PDA/Co-N-CDs nanozyme offers multiple applications in GSH detection and tumor therapy.

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Sensitive and highly selective biosensor based on innovative V₂O₅ nanoparticles for detection of glutathione

Lu,

Xiang,

Ren

2024

Asia-Pacific J Chem Eng

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In this study, V2O5 nanoparticles with flake or prism‐like morphology were synthesized using a two‐step solvothermal synthesis and calcination process for the first time. These nanoparticles exhibited intrinsic oxidase‐like activity, catalyzing the oxidation of 3,3′,5,5′‐tetramethylbenzidine to produce blue oxidized 3,3′,5,5′‐tetramethylbenzidine even in the absence of H2O2, with a characteristic absorption peak at 652 nm. Upon the introduction of glutathione (GSH), the solution color gradually lightened, correlating with a reduction in absorbance. Leveraging these properties, we developed a simple, sensitive, and highly selective colorimetric biosensor utilizing V2O5 nanoparticles for GSH detection in human serum. The developed method demonstrated excellent linearity over a range of 1–30 μM, with a low detection limit of 4.04 nM. Additionally, it exhibited outstanding selectivity against common interfering substances in human serum. Furthermore, this biosensor enabled both naked‐eye detection and spectrophotometric quantitative analysis of GSH. Successful application to spiked serum samples yielded recoveries ranging from 97.1% to 101.7%. Overall, this method offers a promising approach for determining GSH content in human serum, with significant potential for biomedical testing applications. Its rapid and accurate detection capability may contribute to early diagnosis and treatment of various fatal diseases, including cancer and cardiovascular diseases.

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Determination of glutathione and glutathione disulfide using liquid chromatography: A review on recent applications

Cited by 14 publications

References 85 publications

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

MXene/Carbon Dots Nanozyme Composites for Glutathione Detection and Tumor Therapy

Sensitive and highly selective biosensor based on innovative V₂O₅ nanoparticles for detection of glutathione

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Determination of glutathione and glutathione disulfide using liquid chromatography: A review on recent applications

Cited by 14 publications

References 85 publications

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

MXene/Carbon Dots Nanozyme Composites for Glutathione Detection and Tumor Therapy

Sensitive and highly selective biosensor based on innovative V2O5 nanoparticles for detection of glutathione

Contact Info

Product

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Sensitive and highly selective biosensor based on innovative V₂O₅ nanoparticles for detection of glutathione