Determination of glycerol by oxidation with periodic acid

Bradford, Purdy; Pohle, W. D.; Gunther, J. K.; Mehlenbacher, V. C.

doi:10.1007/bf02545480

Cited by 36 publications

(13 citation statements)

References 9 publications

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“…Glycerol was assayed by a modified titration method of the previous report [19]. Ethanol and PDO were analyzed by a gas chromatograph (Shanfen GC112A, Shanghai, China) equipped with a 2-m column filled with Chromosorb 101 (Dikma, Shanghai, China) and a flame ionization detector.…”

Section: Methodsmentioning

confidence: 99%

Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture

Xue

et al. 2010

J Ind Microbiol Biotechnol

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In fed-batch culture of Klebsiella pneumoniae, 1,3-propanediol production was growth associated, while the by-products, including lactic acid and ethanol, increased sharply as the cells grew slowly. When the fed-batch culture was supplied with a mixture of organic acids including citrate, fumarate and succinate, cell growth and 1,3-propanediol production increased significantly, whereas the by-products, especially lactic acid and ethanol, decreased sharply. High concentrations of PDO and acetate inhibited cell growth and PDO production. To improve the PDO production, repeated fed-batch culture with addition of the organic acid mixture was performed in a 5-l reactor. The fed-batch culture was repeated five times, and the 1,3-propanediol yield and concentration reached above 0.61 mol mol(-1) and 66 g l(-1), respectively, in 20 h for each cycle. Furthermore, the PDO productivity reached above 3.30 g l(-1) h(-1) in each cycle, which was much higher than that of the original fed-batch culture.

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Section: Methodsmentioning

confidence: 99%

Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture

Xue

et al. 2010

J Ind Microbiol Biotechnol

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“…2 following the protocol of Kuzndedelov and Severinov (2009) and were stored in aliquots at −20°C until further use. Protein concentration was quantified using Bradford assay with BSA as a standard (Bradford, Pohle, Gunther, & Mehlenbacher, 1942).…”

Section: Purification Of Single Subunits and Transcription Factorsmentioning

confidence: 99%

Recombinant RNA Polymerase from Geobacillus sp. GHH01 as tool for rapid generation of metagenomic RNAs using in vitro technologies

Kinfu

Jahnke

Janus

et al. 2017

Biotech & Bioengineering

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The exciting promises of functional metagenomics for the efficient discovery of novel biomolecules from nature are often hindered by factors associated with expression hosts. Aiming to shift functional metagenomics to a host independent innovative system, we here report on the cloning, heterologous expression, and reconstitution of an RNA polymerase (RNAP) from the thermophilic Geobacillus sp. GHH01 and in vitro transcription thereafter. The five genes coding for RNAP subunits, a house keeping sigma factor and two transcription elongation factors were cloned and over expressed as His -tagged and/ or tag-free proteins. Purified subunits were reconstituted into a functional polymerase through either the classical method of denaturation and subsequent renaturation or through a new resource and time efficient thermo-reconstitution method which takes advantage of the subunits' temperature stability. Additionally, all subunits were cloned into a single vector system for a co-expression and in vivo reconstitution to the RNAP core enzyme. Both the core and holoenzyme form of the RNAP exhibited a robust transcription activity and were stable up to a temperature of 55°C close to their fullest activity. The Geobacillus RNAP showed a remarkable in vitro transcription profile recognizing DNA template sequences of diverse bacteria and archaea as well as metagenomic samples. Coupled with a subsequent in vitro translation step, this recombinant transcription system could allow a new, clone-free, and functional metagenomic screening approach.

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“…The use of periodic acid in glycerol analysis has been reported in several previous papers (1,3,4,5). The equation for the reaction of periodic acid with glycerol is given below:…”

mentioning

confidence: 93%

Free glycerol in soap

Pohle¹,

Rockwood²,

Mehlenbacher³

1949

J Americ Oil Chem Soc

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Summary The determination of free glycerol in soap has been improved and shortened. When it is known that there are no substances which interfere with periodic acid reaction, the directiodimetric method is applicable. However, if the glycerol content is below 1%, the accuracy and precision are only fair. The extraction‐iodimetric method is applicable when the sample contains no periodic acid reducing substance other than glycerol or when such substances are soluble in chloroform. The extraction‐acidimetric method is preferable when the sample contains periodic acid reducing substances other than glycerol and which are soluble in the aqueous acid solution along with the glycerol.

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Determination of glycerol by oxidation with periodic acid

Cited by 36 publications

References 9 publications

Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture

Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture

Recombinant RNA Polymerase from Geobacillus sp. GHH01 as tool for rapid generation of metagenomic RNAs using in vitro technologies

Free glycerol in soap

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