Determination of glycogen with the anthrone reagent

Roe, Joseph H.; Dailey, Robert E.

doi:10.1016/0003-2697(66)90028-5

Cited by 312 publications

(102 citation statements)

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“…A direct relationship between breast carcinoma and cholesterol metabolism has not been confirmed yet. However, this is not the case in the carcinoma of the colon and lung and in haematological neoplasia, where decrease in its serum concentration has been confirmed by several authors (Dessi et al 1992;Rose et al 1974;Spiegel et al 1982). In our experiment, no changes in the serum lipids in the carcinogen (NMU)-influenced animals were observed.…”

Section: Discussionsupporting

confidence: 63%

“…In the serum of the mixed blood, following parameters were determined: the concentration of triacylglycerols (TG), cholesterol (CH), phospholipids (PL) malondialdehyde (MDA) (as a measure of lipid peroxidation) and glucose; in the liver, the contents of TG, PL, CH, MDA and glycogen; in the bone marrow the concentrations of TG, PL, MDA; in the thymus only the content of MDA; in the heart muscle the glycogen content. The concentration of phospholipids was determined from the lipid phosphorus by Bartlett's method (1959), total cholesterol according to Zlatkis et al (1953), glycogen by the method of Roe and Dailey (1966), malondialdehyde in a reaction with thiobarbituric acid (Satch 1978), triacylglycerols and glucose by the commercial sets (Lachema).…”

Section: Methodsmentioning

confidence: 99%

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Chemoprevention of N-Methyl-N-Nitrosourea Induced Mammary Carcinogenesis with Raloxifene and Melatonin: Metabolic Changes in Female Rats

M¹,

Kubatka²,

Kalicka³

et al. 2002

Acta Vet. Brno

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Chamilová M., P. Kubatka, K. Kalická, E. Adámeková, B. Bojková, I. Ahlers, E. Ahlersová: Chemoprevention of N-methyl-N-nitrosourea Induced Mammary Carcinogenesis with Raloxifene and Melatonin: Metabolic Changes in Female Rats. Acta Vet. Brno 2002, 71: 235-242.The aim of this work was to determine the selected parameters of carbohydrate and lipid metabolism in the mammary carcinogenesis induced with N-methyl-N-nitrosourea (NMU) in two doses, each by 50 mg/kg of body weight with a 7-day interval between them within the postnatal days 43 and 54 in female Sprague-Dawley rats. Chemoprevention started with the administration of melatonin (MEL, 4 µg/ml in water, from 15.00 h to 08.00 h) 12 days and raloxifene (RAL 5 mg/kg, 2 × weekly) 10 days before the application of NMU. Twenty-four weeks following the NMU administration the animals were killed, and the incidence, latency, frequency and volume of tumours were evaluated. The animals were divided into: tumour-bearing (TB) and non-tumourbearing (NTB) with the influence of RAL, MEL and their combination. While RAL and RAL plus MEL significantly decreased the incidence and frequency of tumours, the effect of isolated MEL was substantially lower. In the serum, an increase in the concentration of serum glucose in TB and also NTB animals was observed. In the liver of both the TB and NTB animals, the content of cholesterol (CH) and triacylglycerols (TG) decreased and the contents of phospholipids (PL) increased. RAL decreased the contents of CH and PL in the liver of NTB animals and increased the concentration of TG in both groups of animals. Administration of RAL to NTB animals decreased the concentrations of malondialdehyde (MDA) in the serum and thymus, in the bone marrow also in TB animals. MEL decreased the concentration of MDA in the bone marrow of TB animals. MEL increased the concentrations of serum glucose and glycogen content in the heart muscle of NTB animals. RAL plus MEL decreased the concentration of serum TG and PL and decreased the contents of CH and PL in the liver of TB as well as NTB animals. In the thymus and liver, combination of RAL+MEL decreased the MDA content compared with the RAL alone in NTB animals.The co-effect of two or more substances will be probably the optimal way in prevention of cancer. The co-effect of RAL and MEL shows to be a prospective way for influencing the mammary tumors. Breast cancer, female rats, raloxifene, melatonin, chemopreventionThe hormonal therapy of the breast carcinoma is an inseparable part of the variety of therapeutic procedures. The substitution with estrogens has been considered for a long time as a dominant indication of therapy in postmenopausal women, and it has been recognized that approximately one third of women will have a benefit of this procedure. The use of estrogens protects these women against osteoporosis and decreases the cardiovascular risk, but on the other hand, increases the risk of breast and endometrium carcinoma (Col et al. 1997). With regard to the unfavourable effects of estrogens on ...

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Section: Discussionsupporting

confidence: 63%

Section: Methodsmentioning

confidence: 99%

Chemoprevention of N-Methyl-N-Nitrosourea Induced Mammary Carcinogenesis with Raloxifene and Melatonin: Metabolic Changes in Female Rats

M¹,

Kubatka²,

Kalicka³

et al. 2002

Acta Vet. Brno

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“…Basic metabolic and hormonal parameters were determined in serum and selected organs: serum concentration of glucose (GLU); serum and liver concentration of triacylglycerols (TAG), cholesterol (CH), and phospholipids (PL); liver and heart muscle glycogen (GLY) concentration; liver and thymus malondialdehyde (MDA) concentration; serum corticosterone (CTS), insulin (INS), and IGF-1 concentration. GLU and TG were measured using commercial sets (Lachema, Brno, Czech Republic), INS and IGF-1 were determined using commercial RIA sets (Linco Research, St Charles, MO, USA and DRG Instruments GmbH, Germany, respectively), PL were measured from lipid phosphorus according to Bartlett et al [20], CH according to Zlatkis et al [21], GLY according to Roe and Dailey [22], MDA was measured in reaction with thiobarbituric acid according to Satch [23], CTS was measured using fluorimetry according to Guillemin et al [24]. The following basic parameters of mammary carcinogenesis were evaluated in each group: tumor incidence (as the percentage of tumor-bearing animals per group), tumor frequency (as the average number of tumors per group), tumor volume, and latency (the period from carcinogen administration to the appearance of first tumor).…”

Section: Methodsmentioning

confidence: 99%

Metformin in chemically-induced mammary carcinogenesis in rats

Bojková¹,

Orendáš²,

Garajová³

et al. 2009

neo

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In this paper the chemopreventive effect of peroral antidiabetic metformin in mammary carcinogenesis in female SpragueDawley rats was evaluated. Mammary carcinogenesis was induced by N-methyl-N-nitrosourea (NMU) administered in two intraperitoneal doses each per 50 mg/kg b.w. between 43.-55. postnatal days. Metformin was administered in drinking water (at a concentration of 50 µg/ml and 500 µg/ml) 13 days before the first NMU dose until the termination of the experiment. During the experiment the animals were weekly weighed and palpated for the presence of mammary tumors, the incidence, latency, tumor frequency, and tumor volume were recorded. The experiment was terminated 18 weeks after the first NMU dose, basic tumor growth parameters and metabolic and hormonal variables were evaluated. Metformin did not significantly alter the tumor growth although a delay in tumor onset was recorded after higher metformin dose. Metformin altered metabolic and hormonal variables. Insulinemia decreased after both metformin doses in comparison with intact rats without changes in glycemia, triacylglycerols concentration was decreased in liver and increased in serum when compared to intacts. Higher metformin dose attenuated lipoperoxidation in liver.

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“…Glycogen was measured with anthrone reagent after extraction from skeletal muscle with 1 mol/l NaOH at 100°C for 60 min (29).…”

Section: Generation Of G M -Deficient (G Mmentioning

confidence: 99%

Disruption of the Striated Muscle Glycogen Targeting Subunit PPP1R3A of Protein Phosphatase 1 Leads to Increased Weight Gain, Fat Deposition, and Development of Insulin Resistance

et al. 2003

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Determination of glycogen with the anthrone reagent

Cited by 312 publications

References 4 publications

Chemoprevention of N-Methyl-N-Nitrosourea Induced Mammary Carcinogenesis with Raloxifene and Melatonin: Metabolic Changes in Female Rats

Chemoprevention of N-Methyl-N-Nitrosourea Induced Mammary Carcinogenesis with Raloxifene and Melatonin: Metabolic Changes in Female Rats

Metformin in chemically-induced mammary carcinogenesis in rats

Disruption of the Striated Muscle Glycogen Targeting Subunit PPP1R3A of Protein Phosphatase 1 Leads to Increased Weight Gain, Fat Deposition, and Development of Insulin Resistance

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