Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium based on two improved methods

Xiong, Yi; Wang, Boya; Zhou, Chao; Chen, Huan; Chen, Gang; Tang, Youneng

doi:10.1007/s11783-022-1567-y

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Figure 4 compares the 1,4-dioxane biodegradation kinetic parameters determined in this study to the same parameters reported in the literature for all 1,4-dioxane metabolizing mixed cultures ( Zenker et al 2002 ; Mahendra & Alvarez-cohen 2006 ; Nam et al 2016 ; Barajas-Rodriguez & Freedman 2018 ; Lee et al 2020 ; Xiong et al 2022 ) and pure cultures ( Mahendra & Alvarez-cohen 2006 ; Sei et al 2013a ; Chen et al 2016 ; He et al 2017a ; Barajas-Rodriguez & Freedman 2018 ; Inoue et al 2018 ; Yamamoto et al 2018 ; Ma et al 2021 ; Simmer et al 2021 ). Figure 5 further compares the kinetic parameters of Afipia and Pseudonocardia isolated in this study and the literature.…”

Section: Resultsmentioning

confidence: 64%

“…Figures 4 and 5 show the same comparison results: compared to the 1,4-dioxane-metabolizing cultures in the literature, the five pure cultures have lower half-maximum rate constants, higher biomass yields, lower maximum specific 1,4-dioxane utilization rates, and lower decay coefficients. These are characteristics of microorganisms living in oligotrophic environments ( Koch 2001 ; Xiong et al 2022 ; Tang 2023 ).…”

Section: Resultsmentioning

confidence: 99%

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Isolation and characterization of pure cultures for metabolizing 1,4-dioxane in oligotrophic environments

Tesfamariam,

Ssekimpi,

Hoque

et al. 2024

Water Science &Amp; Technology

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1,4-Dioxane concentration in most contaminated water is much less than 1 mg/L, which cannot sustain the growth of most reported 1,4-dioxane-metabolizing pure cultures. These pure cultures were isolated following enrichment of mixed cultures at high concentrations (20 to 1,000 mg/L). This study is based on a different strategy: 1,4-dioxane-metabolizing mixed cultures were enriched by periodically spiking 1,4-dioxane at low concentrations (≤1 mg/L). Five 1,4-dioxane-metabolizing pure strains LCD6B, LCD6D, WC10G, WCD6H, and WD4H were isolated and characterized. The partial 16S rRNA gene sequencing showed that the five bacterial strains were related to Dokdonella sp. (98.3%), Acinetobacter sp. (99.0%), Afipia sp. (99.2%), Nitrobacter sp. (97.9%), and Pseudonocardia sp. (99.4%), respectively. Nitrobacter sp. WCD6H is the first reported 1,4-dioxane-metabolizing bacterium in the genus of Nitrobacter. The net specific growth rates of these five cultures are consistently higher than those reported in the literature at 1,4-dioxane concentrations <0.5 mg/L. Compared to the literature, our newly discovered strains have lower half-maximum-rate concentrations (1.8 to 8.2 mg-dioxane/L), lower maximum specific 1,4-dioxane utilization rates (0.24 to 0.47 mg-dioxane/(mg-protein ⋅ d)), higher biomass yields (0.29 to 0.38 mg-protein/mg-dioxane), and lower decay coefficients (0.01 to 0.02 d−1). These are characteristics of microorganisms living in oligotrophic environments.

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Section: Resultsmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of pure cultures for metabolizing 1,4-dioxane in oligotrophic environments

Tesfamariam,

Ssekimpi,

Hoque

et al. 2024

Water Science &Amp; Technology

Self Cite

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“…Thierrin et al have documented BTEX biodegradation under sulfate-reducing condition resulting from a gasoline spill [ 9 ]. Thus, the potential utilization of sulfate-reducing processes for the remediation of BTEX-contaminated groundwater presents a practical and cost-effective remedial option for addressing petroleum hydrocarbon contamination [ 4 , 10 , 11 , 12 , 13 , 14 , 15 ]. Several studies have detailed the contaminant removal performance achieved through sulfate-reducing processes.…”

Section: Introductionmentioning

confidence: 99%

Shift in Bacterial Community Structure in the Biodegradation of Benzene and Toluene under Sulfate-Reducing Condition

Liu,

Lin,

Wang

et al. 2024

Toxics

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Groundwater contaminated by benzene and toluene is a common issue, posing a threat to the ecosystems and human health. The removal of benzene and toluene under sulfate-reducing condition is well known, but how the bacterial community shifts during this process remains unclear. This study aims to evaluate the shift in bacterial community structure during the biodegradation of benzene and toluene under sulfate-reducing condition. In this study, groundwater contaminated with benzene and toluene were collected from the field and used to construct three artificial samples: Control (benzene 50 mg/L, toluene 1.24 mg/L, sulfate 470 mg/L, and HgCl2 250 mg/L), S1 (benzene 50 mg/L, toluene 1.24 mg/L, sulfate 470 mg/L), and S2 (benzene 100 mg/L, toluene 2.5 mg/L, sulfate 940 mg/L). The contaminants (benzene and toluene), geochemical parameters (sulfate, ORP, and pH), and bacterial community structure in the artificial samples were monitored over time. By the end of this study (day 90), approximately 99% of benzene and 96% of toluene could be eliminated in both S1 and S2 artificial samples, while in the Control artificial sample the contaminant levels remained unchanged due to microbial inactivation. The richness of bacterial communities initially decreased but subsequently increased over time in both S1 and S2 artificial samples. Under sulfate-reducing condition, key players in benzene and toluene degradation were identified as Pseudomonas, Janthinobacterium, Novosphingobium, Staphylococcus, and Bradyrhizobium. The results could provide scientific basis for remediation and risk management strategies at the benzene and toluene contaminated sites.

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Characterization of 1,4-dioxane degrading microbial community enriched from uncontaminated soil

Tang

Wang

Lee

et al. 2023

Appl Microbiol Biotechnol

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Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium based on two improved methods

Cited by 7 publications

References 27 publications

Isolation and characterization of pure cultures for metabolizing 1,4-dioxane in oligotrophic environments

Isolation and characterization of pure cultures for metabolizing 1,4-dioxane in oligotrophic environments

Shift in Bacterial Community Structure in the Biodegradation of Benzene and Toluene under Sulfate-Reducing Condition

Characterization of 1,4-dioxane degrading microbial community enriched from uncontaminated soil

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