Determination of Guanidino Compounds in Serum by Micellar Electrokinetic Capillary Chromatography Using Benzoin as the Derivatizing Reagent

GC analysis of guanidino compounds in serum and urine of healthy volunteers and uremic patients using methylglyoxal and ethyl chloroformate as derivatizing reagent

“…The effect of pH on second derivatization with ECF was examined within 6-10 at unit intervals and the maximum response was at pH 9 using carbonate buffer, as has been reported for reactions with ECF. 37 The volume of buffer added per analysis was changed from 0.1 to 1.0 mL at, intervals of 0.1 mL, and the change in volume of the buffer did not have an effect on the average response (n ¼ 4), and the volume of the buffer was xed at 0.3 mL. The addition of derivatizing reagent ECF was varied from 0.1 to 0.6 mL at an interval of 0.1 mL, but no change in peak height was observed by changing its volume, and addition of 0.3 mL ECF per analysis was considered optimal.…”

“…The supernatant layer was collected. 37 The deproteinized serum 1 mL was transferred to a screw capped vial and the derivatization and GC analysis were carried out using an analytical procedure. The quantitation was carried out using external linear calibration curves using the linear regression equation Y ¼ ax + b.…”

“…Ethyl chloroformate (ECF) is reported to react with carboxylic acid and imine groups quickly in the aqueous-organic phase to form derivatives compatible with GC. [37][38][39] The present work examines the use of MGo and ECF as precolumn derivatizing reagents for GC elution, separation and determination of the guanidino compounds from biological uids (serum and urine). Experimental conditions for derivatization, GC elution and separation are optimized before determination.…”

Improved Gas Chromatographic Determination of Guanidino Compounds Using Isovaleroylacetone and Ethyl Chloroformate as Derivatizing Reagents

“…The analytical procedures are mainly based on high performance liquid chromatography (HPLC), 5,[9][10][11][12][13][14][15][16] gas chromatography (GC) 6,[17][18][19][20] and capillary electrophoresis. [21][22] HPLC is more frequently used for the analysis of guanidino compounds, 4 but capillary GC has high potential for the separation and analysis of volatile organic compounds with short analysis time. The guanidino compounds have been converted to volatile species by derivatization with hexaflouroacetylacetone (HFAA) and trimethylsilyltrifluoroacetamide, 6,19 HFAA and trifluoroacetic anhydride, 23 HFAA and pentfluorobenzyl bromide 20 or HFAA and ethylchloroformate.…”

Gas Chromatographic Analysis of Guanidino Compounds in Sera and Urine of Uremic Patients Using Glyoxal and Ethyl Chloroformate as Derivatizing Reagents

“…20 Benzoin has also been used as a pre capillary derivatization reagent for guanidino compounds using micellar electrokinetic capillary chromatography. 21 The required sensitivity is achieved using HPLC but HPLC suffers from the problem of safe disposal of used solvents.…”