Determination of Heavy Metal Levels, Oxidative Status, Biochemical and Hematological Parameters in Cyprinus carpio L., 1758 from Bafra (Samsun) Fish Lakes

Kandemir, Şevket; Dogru, M. I.; Örün, İbrahim; Doğru, Arzu; Altaş, Levent; Erdoğan, Kenan; Örün, Gül Nihal; Polat, Nazmi

doi:10.3923/javaa.2010.617.622

Cited by 28 publications

(15 citation statements)

References 20 publications

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“…In contrast, copper content in muscle was not affected by exposure time; also, the copper concentration was lowest in muscle tissue compared to the other samples. Overall, we observed a copper distribution in tissues comparable to the findings of other studies (De Boeck et al 2004;Celechovska et al 2007;Kandemir et al 2010).…”

Section: Discussionsupporting

confidence: 90%

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Ševčíková¹,

Modrá²,

Blahová³

et al. 2016

Vet. Med.

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ABSTRACT:The aim of the present study was to investigate the impact of copper-based pesticides (at concentrations of copper of 20, 30, 40 and 70 µg/l) on one-year-old common carp (Cyprinus carpio L.) during 28 days of exposure. Abnormal behaviour was observed in fish exposed to 70 µg/l from Day 14. Histological alterations were noticed only in liver in the groups exposed to 40 and 70 µg/l. Significant changes (P ˂ 0.05) in almost all haematological indices were found, especially in the group exposed to the highest concentration of copper (70 µg/l). Biochemical analysis revealed various significant (P ˂ 0.05) differences among the tested groups. Significant differences in copper tissue concentration (P ˂ 0.05) among groups were found in liver, gills and kidney. Among antioxidative enzymes, significant changes were revealed mainly in catalase and glutathione-S-transferase activity (P ˂ 0.05). In gills, metallothionein content increased significantly (P ˂ 0.05) in the group exposed to the highest copper concentration (70 µg/l) compared with the other tested groups, including the control. A significant (P ˂ 0.05) change in total glutathione content was recorded in liver and gills, although the reduced/oxidised ratio was not affected. Oxidative damage to lipids increased significantly (P ˂ 0.05) with increasing copper concentration in liver and kidney. The results demonstrate the deleterious influence of copper on common carp even at low, environmentally relevant concentrations.

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Section: Discussionsupporting

confidence: 90%

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Ševčíková¹,

Modrá²,

Blahová³

et al. 2016

Vet. Med.

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“…Apart from the above mentioned metals and metalloids, other metals are also connected with oxidative stress (nickel, vanadium, and cobalt) (Stohs and Bagchi, 1995) and can be detected in aquatic environments (Kandemir et al, 2010). Tributyltin and aluminium are widespread pollutants.…”

Section: Other Metalsmentioning

confidence: 99%

Metals as a cause of oxidative stress in fish: a review

Ševčíková¹,

Modrá²,

Slaninova³

et al. 2011

Vet. Med.

319

123

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ABSTRACT:This review summarizes the current knowledge on the contribution of metals to the development of oxidative stress in fish. Metals are important inducers of oxidative stress in aquatic organisms, promoting formation of reactive oxygen species through two mechanisms. Redox active metals generate reactive oxygen species through redox cycling, while metals without redox potential impair antioxidant defences, especially that of thiol-containing antioxidants and enzymes. Elevated levels of reactive oxygen species lead to oxidative damage including lipid peroxidation, protein and DNA oxidation, and enzyme inactivation. Antioxidant defences include the enzyme system and low molecular weight antioxidants. Metal-binding proteins, such as ferritin, ceruloplasmin and metallothioneins, have special functions in the detoxification of toxic metals and also play a role in the metabolism and homeostasis of essential metals. Recent studies of metallothioneins as biomarkers indicate that quantitative analysis of mRNA expression of metallothionein genes can be appropriate in cases with elevated levels of metals and no evidence of oxidative damage in fish tissue. Components of the antioxidant defence are used as biochemical markers of oxidative stress. These markers may be manifested differently in the field than in results found in laboratory studies. A complex approach should be taken in field studies of metal contamination of the aquatic environment. Keywords: ROS; metallothioneins; glutathion; superoxide dismutase; antioxidant defenceList of abbreviations ALA-D = aminolevulinic acid dehydratase; BNF = β-naphthoflavone; CAT = catalase; GPx = glutathione peroxidise; GR = glutathione reductase; GSH = glutathione; GSSG = glutathione disulphide; GST = glutathione S-transferase; LPO = lipid peroxidation; MDA = malondialdehyde; MTs = metallothioneins; NADPH = nicotinamidadeninedinucleotide phosphate (reduced); ROS = reactive oxygen species; SOD = superoxide dismutase

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“…This may be due to loss of equilibrium at high intoxication which makes the fish to turn upside down and finally died. The swimming performance is considered one measure which could serve as possible sensitive indicator of sub-lethal toxic exposure (Kandemir et al, 2010). Various methods have been used to quantify the effects of toxics on an organism's swimming performance (Rose et al, 1993).…”

Section: Behavioural Changesmentioning

confidence: 99%

“…kinds of organisms may be used to determine the mechanisms of action of pollutants on specific physiological function (Gul et al, 2004;Kandemir et al, 2010). According to Eisler and Gardener (1973) heavy metals are being passed on into aqueous environments through industrial processes, sewage disposal, soil leaching and rainfall.…”

Section: Introductionmentioning

confidence: 99%

Impact of nickel (Ni) on hematological parameters and behavioral changes in Cyprinus carpio (common carp)

Al‐Ghanim¹

2011

Afr. J. Biotechnol.

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The effect of nickel on hematological parameters and behaviour in Cyprinus carpio after a 96 h exposure to nickel test was investigated. Cyprinus carpio fingerlings were obtained from local fish hatchery. Morphometric characteristics of experimental fish were recorded. Fish of both sexes were stocked without discrimination. The fish were exposed to different concentrations (0, 6, 9, 12, 15 and 18 mg/l) of nickel sulphate using standard screening procedure. The mortality rate of the experimental fish was increased with increase in concentration of nickel. The 96 h median lethal concentration (96 h LC50) was 12.44 mg Ni/L using the logarithmic method with dose-mortality regression line y = 188.224x -86.52. The dissolved oxygen concentration decreased with increase in the level of Ni. All the blood parameters (erythrocyte, leucocytes, hematocrit and hemoglobin count) decreased with increasing dose of nickel and become significantly lower (P < 0.05) at higher concentration when compared with the control. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were also lowered with concentration of toxicant when compared with the control. The results of the present study showed that a short-term exposures to high levels of nickel induced stress reactions in C. carpio. Some adaptive changes were observed; preparing the organism to an increased energy expense, whereas other changes showed a considerable immunosuppressive effect of stress. It was concluded that changes observed indicate that hematological parameters can be used as an indicator of Ni stress in C. carpio.

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Determination of Heavy Metal Levels, Oxidative Status, Biochemical and Hematological Parameters in Cyprinus carpio L., 1758 from Bafra (Samsun) Fish Lakes

Cited by 28 publications

References 20 publications

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Metals as a cause of oxidative stress in fish: a review

Impact of nickel (Ni) on hematological parameters and behavioral changes in Cyprinus carpio (common carp)

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