Determination of heavy polycyclic aromatic hydrocarbons of concern in edible oils via excitation–emission fluorescence spectroscopy on nylon membranes coupled to unfolded partial least-squares/residual bilinearization

Abstract

Seven heavy polycyclic aromatic hydrocarbons (PAHs) of concern on the US Environmental Protection Agency priority pollutant list (benzo[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenz[a,h]anthracene, benzo[g,h,i]perylene, and indeno[1,2,3-c,d]-pyrene) were simultaneously analyzed in extra virgin olive oil. The analysis is based on the measurement of excitation-emission matrices on nylon membrane and processing of data using unfolded partial least-squares regression with residual bilinearization (U-PLS/RBL). The conditions needed to retain the PAHs present in the oil matrix on the nylon membrane were evaluated. The limit of detection for the proposed method ranged from 0.29 to 1.0 μg kg(-1), with recoveries between 64 and 78 %. The predicted U-PLS/RBL concentrations compared favorably with those measured using high-performance liquid chromatography with fluorescence detection. The proposed method was applied to ten samples of edible oil, two of which presented PAHs ranging from 0.35 to 0.63 μg kg(-1). The principal advantages of the proposed analytical method are that it provides a significant reduction in time and solvent consumption with a similar limit of detection as compared with chromatography.

Formation of Benzo(a)pyrene in Sesame Seeds During the Roasting Process for Production of Sesame Seed Oil

“…However, BaP may be produced when SS is roasted at high temperatures. Up till now, attention have been centered on the determination of methods and on the elimination of BaP during oil refining [17][18][19], and it has been confirmed that BaP is generated during roasting of coffee, breads and meat [8,20,21], and a high level of BaP has been detected in crude SSO [22]. During the roasting process, BaP content may depend on the both temperature and time.…”

Usage of Cellulose Diacetate as Sorption Material for Fluorescent Analysis of PAH

“…The application of SPL enables one to combine solid-phase extraction with getting a signal in the sorbent phase [4][5][6][7][8][9][10] . This method allows observing the fluorescence of substances previously adsorbed on solid matrices, just at room temperature.…”

Determination of benzo[a]pyrene in camellia oil via vortex-assisted extraction using the UPLC-FLD method

“…Triglyceride is a major matrix interference associated with the determination of benzo[a]pyrene (12). Saponification and solidphase extraction (SPE) are the most widely used cleanup methods.…”