Determination of heparan sulphate in kidney tissues of patients with calcium nephrolithiasis

Chan, Virginia; Tan, Eek Chaw; Li, M. K.

doi:10.1007/bf00300024

Cited by 5 publications

(3 citation statements)

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“…However, the level of HS-PG mRNA expression was significantly higher in SF rats than in normal rats. Basement membrane HS-PG levels in urolithiasis were investigated by Chan and Tan [5], who reported a significant decrease of HS-PG in the GBM and upper urinary tract. However, they did not find significant changes in HS-PG in the TBM between patients with stones and normal controls [5].…”

Section: Discussionmentioning

confidence: 99%

“…Basement membrane HS-PG levels in urolithiasis were investigated by Chan and Tan [5], who reported a significant decrease of HS-PG in the GBM and upper urinary tract. However, they did not find significant changes in HS-PG in the TBM between patients with stones and normal controls [5]. Nishio et al [17] also reported that HS levels increased in rat renal tubules during microlith formation.…”

Section: Discussionmentioning

confidence: 99%

“…However, the reason for this remains unclear. Chan and Tan [5] investigated the levels of heparan sulfate proteoglycan (HS-PG) in tissues from calcium nephrolithiasis patients and in those from normal controls. They found that HS-PG levels apparently did not change in the tubular basement membrane.…”

Section: Introductionmentioning

confidence: 99%

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Expression of heparan sulfate proteoglycan mRNA in rat kidneys during calcium oxalate nephrolithiasis

et al. 1997

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This study used reverse transcription polymerase chain reaction (RT-PCR) to examine heparan sulfate proteoglycan (HS-PG) mRNA expression levels during stone formation in the rat kidney. Total RNA in kidneys was extracted and converted to cDNA. PCR products were resolved by electrophoresis on 1.5% agarose gel and visualized with ethidium bromide. Fragment intensity and area were measured using an image analyzer. Control cyclophilin and HS-PG mRNAs were expressed in all samples examined as 235 bp and 506 bp bands, respectively. Cyclophilin expression in the normal group was not significantly different from expression in the group that formed stones. However, the level of HS-PG mRNA expression apparently increased in calcium oxalate (CaOx) microlith. The findings suggest an association between CaOx nephrolithiasis and expression of HS-PG in the rat kidney.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression of heparan sulfate proteoglycan mRNA in rat kidneys during calcium oxalate nephrolithiasis

et al. 1997

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Heparan sulfate gene polymorphism in calcium oxalate nephrolithiasis

et al. 2008

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Calcium oxalate (CaOx) nephrolithiasis has a complex pathogenic mechanism. Besides environmental factors, genetic factors also have influence on stone formation. This study represents the effects of heparan sulfate (HSPG2) gene polymorphism for determining the risk of urolithiasis. We investigated 143 CaOx stone formers with 158 healthy individuals for the BamHI restriction site polymorphism located in intron 6 of the HSPG gene using the polymerase chain reaction, restriction fragments length polymorphism method. After digestion with BamHI, the polymorphism was assumed to cause three genotypes according to the banding types as GG (242 bp), GT (242, 144, and 98 bp) and TT (144 and 98 bp). According to the genotype frequencies between the groups, TT genotype showed significantly increased risk for urolithiasis than TG and GG genotypes. We concluded that HSPG2 gene polymorphism might be one of the genetic factors affecting the CaOx stone formation.

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Quantitative analysis on the localization of chondroitin sulfate proteoglycan in renal tissues of patients with calcium nephrolithiasis

Chan

1998

Urological Research

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Previous studies have shown a significant decrease of heparin sulfate proteoglycan (HSPG) in the basement membrane of the glomerulus and the mucosa of the ureter/renal pelvis in patients with calcium nephrolithiasis. In this study, we looked at the localization of another influential proteoglycan, chondroitin sulfate (CSPG), using similar study groups by indirect immunofluorescence staining. Microscopic images were digitized and image analysis was used to quantitate the staining intensity of CSPG present in the basement membrane of the nephron. Our data showed significant loss of CSPG in the Bowman's capsule and the basement membrane of the mucosa of the ureter/renal pelvis using Mann-Whitney U-Wilcoxon Rank Sum W test with P-values of 0.0043 and 0.0041, respectively. However, absence of staining was noted in the basement membrane of the glomerulus and no significant change in the basement membrane of the tubular epithelium was observed. In conclusion, our results showed changes in the localization of CSPG in the basement membrane of the nephron, accompanied with HSPG, which may contribute to the pathological condition of calcium nephrolithiasis.

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Determination of heparan sulphate in kidney tissues of patients with calcium nephrolithiasis

Cited by 5 publications

References 9 publications

Expression of heparan sulfate proteoglycan mRNA in rat kidneys during calcium oxalate nephrolithiasis

Expression of heparan sulfate proteoglycan mRNA in rat kidneys during calcium oxalate nephrolithiasis

Heparan sulfate gene polymorphism in calcium oxalate nephrolithiasis

Quantitative analysis on the localization of chondroitin sulfate proteoglycan in renal tissues of patients with calcium nephrolithiasis

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