Determination of homocysteine and other low-molecular-weight amino thiols in blood plasma

Recent advances in separation and detection methods for thiol compounds in biological samples

“…Several aminothiols were analyzed by LC-FL using these reagents under mild reaction conditions. 5-or 6-IAF (5-or 6-iodoacetamidofluorescein) is one of the aliphatic halide-containing reagents for thiol labeling [75][76][77][78][79][80][81]. The reaction scheme of 5-IAF with Pen is shown in Fig.…”

Fully automated method for simultaneous determination of total cysteine, cysteinylglycine, glutathione and homocysteine in plasma by HPLC with UV absorbance detection

“…Chromatographic methods have been developed extensively for total homocysteine determinations despite the recent introduction of immunoassays. Among chromatographic methods, gas chromatography coupled to mass spectrometry [9], high performance liquid chromatography [10][11][12][13] and capillary electrophoresis [14] has been developed. To our knowledge only few fully automated procedures for determining total homocysteine and other metabolically related thiols has been described [15][16][17].…”

Capillary electrophoresis in the analysis of biologically important thiols

“…The theoretical knowledge on various thiol separation systems as studied in numerous publications was applied in a wide range of biological samples with clinical importance. Those include thiols in human blood plasma , human whole blood , erythrocytes , carotid atherosclerotic plaques , cultured cells , and bacteria . Except for endogenous thiols, also pharmaceutical compounds containing thiol moiety such as d‐PENA in blood plasma , captopril in urine , and thiouracil used in the treatment of hyperthyroidism were analyzed after 5‐IAF derivatization.…”