Determination of Hydrocortisone in Pharmaceutical Drug by TLC With Densitometric Detection in Uv

Pyka, Alina; Babuška, Robert; Bocheńska, Paulina

doi:10.1080/10826076.2011.563891

Cited by 15 publications

(8 citation statements)

References 25 publications

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“…In our previous paper [ 19 ], we confirmed that normal phase thin-layer chromatography (NP-TLC) combined with densitometry is a method which can be successfully applied for identification and quantification of hydrocortisone in a simple pharmaceutical formulation in form of tablets containing 20 mg of hydrocortisone per tablet. We found that the optimal separation of hydrocortisone from its related substances: hydrocortisone acetate and prednisolone can be achieved on silica gel 60F 254 and by the use of mobile phase: acetone + n -hexane + glacial acetic acid in volume composition 5 mL: 5 mL: 0.05 mL.…”

Section: Resultsmentioning

confidence: 53%

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Dołowy

Kulpińska-Kucia

Pyka

2014

The Scientific World Journal

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A new specific, precise, accurate, and robust TLC-densitometry has been developed for the simultaneous determination of hydrocortisone acetate and lidocaine hydrochloride in combined pharmaceutical formulation. The chromatographic analysis was carried out using a mobile phase consisting of chloroform + acetone + ammonia (25%) in volume composition 8 : 2 : 0.1 and silica gel 60F254 plates. Densitometric detection was performed in UV at wavelengths 200 nm and 250 nm, respectively, for lidocaine hydrochloride and hydrocortisone acetate. The validation of the proposed method was performed in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, and robustness. The applied TLC procedure is linear in hydrocortisone acetate concentration range of 3.75 ÷ 12.50 μg·spot−1, and from 1.00 ÷ 2.50 μg·spot−1 for lidocaine hydrochloride. The developed method was found to be accurate (the value of the coefficient of variation CV [%] is less than 3%), precise (CV [%] is less than 2%), specific, and robust. LOQ of hydrocortisone acetate is 0.198 μg·spot−1 and LOD is 0.066 μg·spot−1. LOQ and LOD values for lidocaine hydrochloride are 0.270 and 0.090 μg·spot−1, respectively. The assay value of both bioactive substances is consistent with the limits recommended by Pharmacopoeia.

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Section: Resultsmentioning

confidence: 53%

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Dołowy

Kulpińska-Kucia

Pyka

2014

The Scientific World Journal

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“…Literature survey disclose that different methods are available for detection of HYD and CL separately; for HYD, these are polarographic [12,13], HPLC [14,15], spectrophotometric [16], TLC [17], and LC-MS/MS [18,19] methods; and for CL, these are spectrophotometric [20], HPLC methods [21,22], and titrimetric [23] TLC [24] methods. Only one method has been reported for the simultaneous determination of HYD and CL that is HPTLC [25].…”

Section: Introductionmentioning

confidence: 99%

Analytical method development and validation of hydrocortisone and clotrimazole in topical dosage form using RP-HPLC

et al. 2020

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Background: Different pharmaceutical topical agents are available in the market for the treatment of fungal infections. A simple, precise, and cost-effective RP-HPLC method was developed and validated for the determination of hydrocortisone and clotrimazole simultaneously in a topical cream dosage form. Chromatographic separation was done on USP L1 (250 × 4.6) mm column with a particle size of 5 μm. The mobile phase employed for this study consists of acetonitrile and buffer in the ratio of 75:25, respectively. The flow rate was kept at 1 mL per minute. The detection of the drug was carried out at 254 nm using a UV detector. The retention times of HYD and CL were 3.0 min and 7.3 min, respectively. Result: The method is developed and validated for linearity, precision, specificity, accuracy, and robustness. Conclusion: The stability of finished products gives us knowledge about the effect of different environmental factors like humidity, light, and temperature, and these factors give us information about the quality of finished products.

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“…HDS is commonly used to treat inflammatory skin diseases (Greive & Barnes, 2015) and is also administered to stock animals to promote growth. It is used to suppress the immune response (Pyka, Babuska-Roczniak, & Bochenska, 2011) and promote weight gain in animals by reducing energy consumption (Quillet et al, 2014). However, its overuse causes residues to accumulate in animal products, such as milk and pork, which can endanger human health (Chen et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic assay for hydrocortisone residues in milk

Wang

Xie

Cui

et al. 2017

Food and Agricultural Immunology

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An anti-hydrocortisone (HDS) monoclonal antibody, 2G8, based on a HDS succinic anhydride derivative hapten, was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic assay for the detection of HDS in milk samples. The half inhibitory concentration (IC 50) of the antibody was 0.095 ng/mL, its limit of detection was 0.013 ng/mL, its linear range of detection was 0.026-0.356 ng/mL, and its cross-reactivity with HDS analogs was <5%. In spiked samples and a recovery test, the recovery rates ranged from 92% to 98.5%, indicating the suitability of this ic-ELISA for the analysis of HDS in milk. The immunochromatographic strip had a cutoff value of 2 ng/mL in milk and could be used for the semiquantitative analysis of HDS. When milk samples were added to the sample pad of the strip, a bright test line indicated <0.2 ng/mL HDS, a weak test line indicated 0.2-2 ng/mL HDS, and no test line indicated ≥2 ng/mL HDS. Analysis of HDS in milk samples showed that results acquired by the immunochromatographic assay agreed well with results acquired by ic-ELISA. Thus, the ic-ELISA and strip assay developed in this study rapidly and sensitively detect HDS residues in milk samples.

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Determination of Hydrocortisone in Pharmaceutical Drug by TLC With Densitometric Detection in Uv

Cited by 15 publications

References 25 publications

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Analytical method development and validation of hydrocortisone and clotrimazole in topical dosage form using RP-HPLC

Development of an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic assay for hydrocortisone residues in milk

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