Determination of hydrogen sulfide and acid-labile sulfur in animal tissues by gas chromatography and ion chromatography

Ubuka, Toshihiko; Abe, Tadashi; Kajikawa, Rie; Morino, Katsuya

doi:10.1016/s0378-4347(01)00046-9

Cited by 87 publications

(59 citation statements)

References 15 publications

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“…Fifth, compounds frequently employed to inhibit either CSE (propargyl glycine and b-cyanoalanine), CBS (aminooxyacetate), or both (hydroxylamine) and thus used to distinguish the rate and/or pathway of H 2 S production are not specific for these enzymes and they are often poorly absorbed by tissues (67). Sixth, although limited in number, a number of studies have suggested that free H 2 S in tissues is under 50 nM (18,37) or undetectable (73). Some of the potential sources for error in H 2 S chemistry and blood/tissue measurements are summarized in Fig.…”

Section: H 2 S In Tissuementioning

confidence: 99%

A Practical Look at the Chemistry and Biology of Hydrogen Sulfide

Olson

2012

Antioxidants & Redox Signaling

186

179

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Significance: Hydrogen sulfide (H 2 S) is garnering increasing interest as a biologically relevant signaling molecule. The effects of H 2 S have now been observed in virtually every organ system and numerous physiological processes. Recent Advances: These studies have not only opened a new field of ''gasotransmitter'' biology, they have also led to the development of synthetic H 2 S ''donating'' compounds with the potential to be parlayed into a variety of therapeutic applications. Critical Issues: Often lost in the exuberance of this new field is a critical examination or understanding of practical aspects of H 2 S chemistry and biology. This is especially notable in the areas of handling and measuring H 2 S, evaluating biosynthetic and metabolic pathways, and separating physiological from pharmacological responses. Future Directions: This brief review describes some of the pitfalls in H 2 S chemistry and biology that can lead or have already led to misleading or erroneous conclusions. The intent is to allow individuals entering or already in this burgeoning field to critically analyze the literature and to assist them in the design of future experiments. Antioxid. Redox Signal. 17, 32-44.

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Section: H 2 S In Tissuementioning

confidence: 99%

A Practical Look at the Chemistry and Biology of Hydrogen Sulfide

Olson

2012

Antioxidants & Redox Signaling

186

179

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“…However, the reduced divalent form of sulfur is very reactive within biological matrices, resulting in sulfide equivalents being present in various volatile sulfur pools, which includes both free hydrogen sulfide and the bound acidlabile sulfides as well as a sulfane sulfur pool (41). The acidlabile sulfide consists of sulfur present in the iron-sulfur clusters contained in a variety of proteins and enzymes, including rubredoxins, ferredoxins, aconitase, and succinate dehydrogenase (41,42). The sulfane sulfur, divalent sulfur atoms bound only to other sulfur, includes thiosulfate, persulfides, thiosulfonates, polysulfides, polythionates, and elemental sulfur (43).…”

Section: Treatment Of U937 Monocytes and Pbmc With High Glucosementioning

confidence: 99%

Decreased Cystathionine-γ-lyase (CSE) Activity in Livers of Type 1 Diabetic Rats and Peripheral Blood Mononuclear Cells (PBMC) of Type 1 Diabetic Patients

Manna

Güngör

McVie

et al. 2014

Journal of Biological Chemistry

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Background:Mechanism of impaired H 2 S signaling in diabetes is not clear. Results: Livers from type 1 diabetic (T1D) rats and PBMC isolated from T1D patients have lower cystathionine-␥-lyase activity. High glucose and/or high ketone treatment also decreased cystathionine-␥-lyase activity in U937 monocytes and PBMC of healthy subjects. Conclusion: Uncontrolled glycemia and ketosis down-regulate cystathionine-␥-lyase activity in T1D. Significance: Impaired cystathionine-␥-lyase can dysregulate H 2 S signaling in diabetes.

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“…Determination of RSCs in filter-sterilized and heat-treated SRB cultures (medium and soil solution) by ion chromatography RSCs in 0.1 M sodium hydroxide solution were easily oxidized to sulfate by hydrogen peroxide, which could be determined by ion chromatography (Ubuka et al, 2001). The 28-day cultivated SRB medium/soil solution was filter-sterilized and heattreated, then 10 ml solution was added into 500 ml 0.2 M sodium hydroxide solution and placed in a test tube with a Teflon-lined screw cap.…”

Section: Utilization Of Sulfur Compounds By Srbmentioning

confidence: 99%

Dechlorination of p,p′-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp. BXM

Bao

Wang

et al. 2012

Environmental Pollution

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a b s t r a c tA novel non-dsrAB (without dissimilatory sulfite reductase genes) sulfate-reducing bacterium (SRB) Clostridium sp. BXM was isolated from a paddy soil. Incubation experiments were then performed to investigate the formation of reduced sulfur compounds (RSC) by Clostridium sp. BXM, and RSC-induced dechlorination of p,p 0 -DDT in culture medium and soil solution. The RSCs produced were 5.8 mM and 4.5 mM in 28 mM sulfate amended medium and soil solution respectively after 28-day cultivation. The p,p 0 -DDT dechlorination ratios were 74% and 45.8% for 5.8 mM and 4.5 mM RSCs respectively at 6 h. The metabolites of p,p 0 -DDT found in the two reaction systems were identified as p,p 0 -DDD and p,p 0 -DDE. The dechlorination pathways of p,p 0 -DDT to p,p 0 -DDD and p,p 0 -DDE were proposed, based on mass balance and dechlorination time-courses. The results indicated that RSC-induced natural dechlorination may play an important role in the fate of organochlorines.Crown

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Determination of hydrogen sulfide and acid-labile sulfur in animal tissues by gas chromatography and ion chromatography

Cited by 87 publications

References 15 publications

A Practical Look at the Chemistry and Biology of Hydrogen Sulfide

A Practical Look at the Chemistry and Biology of Hydrogen Sulfide

Decreased Cystathionine-γ-lyase (CSE) Activity in Livers of Type 1 Diabetic Rats and Peripheral Blood Mononuclear Cells (PBMC) of Type 1 Diabetic Patients

Dechlorination of p,p′-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp. BXM

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