Determination of<i>Elizabethkingia</i>Diversity by MALDI-TOF Mass Spectrometry and Whole-Genome Sequencing

Eriksen, Helle Brander; Gumpert, Heidi; Faurholt, Cecilie Haase; Westh, Henrik

doi:10.3201/eid2302.161321

Cited by 30 publications

(34 citation statements)

References 15 publications

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“…Previous reports have shown that currently available commercial databases are limited in their ability to identify Elizabethkingia species [15,20]. Therefore, researchers have attempted to differentiate three speciesdE.…”

Section: Discussionmentioning

confidence: 99%

Multicentre study evaluating matrix-assisted laser desorption ionization–time of flight mass spectrometry for identification of clinically isolated Elizabethkingia species and analysis of antimicrobial susceptibility

Cheng

Perng

Jian

et al. 2019

Clinical Microbiology and Infection

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Section: Discussionmentioning

confidence: 99%

Multicentre study evaluating matrix-assisted laser desorption ionization–time of flight mass spectrometry for identification of clinically isolated Elizabethkingia species and analysis of antimicrobial susceptibility

Cheng

Perng

Jian

et al. 2019

Clinical Microbiology and Infection

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“…However, even if the sequencing price has significantly decreased during the past decade, this technology is not implemented in every diagnosis laboratory, and the analysis requires much more time than mass spectrometry. Yet still, very few studies show the tandem utilization of WGS and MALDI-TOF MS [92,93]. Both techniques present advantages and disadvantages but seem to show a particular complementarity.…”

Section: Outlook and Future Challenges For Maldi-tof Ms And Amr In Thmentioning

confidence: 99%

“…Both techniques present advantages and disadvantages but seem to show a particular complementarity. As an example, colony identification of Elizabethkingia spp., a ubiquitous bacteria found both in the environment and hospital settings, was carried out by MALDI-TOF MS [93]. WGS was used for the detection of antimicrobial resistance genes and to confirm MALDI-TOF MS identification.…”

Section: Outlook and Future Challenges For Maldi-tof Ms And Amr In Thmentioning

confidence: 99%

MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens

2019

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Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is today the reference method for direct identification of microorganisms in diagnostic laboratories, as it is notably time-and cost-efficient. In the context of increasing cases of enteric diseases with emerging multi-drug resistance patterns, there is an urgent need to adopt an efficient workflow to characterize antimicrobial resistance (AMR). Current approaches, such as antibiograms, are time-consuming and directly impact the "patient-physician" workflow. Through this mini-review, we summarize how the detection of specific patterns by MALDI-TOF MS, as well as bioinformatics, become more and more essential in research, and how these approaches will help diagnostics in the future. Along the same lines, the idea to export more precise biomarker identification steps by MALDI-TOF(/TOF) MS data towards AMR identification pipelines is discussed. The study also critically points out that there is currently still a lack of research data and knowledge on different foodborne pathogens as well as several antibiotics families such as macrolides and quinolones, and many questions are still remaining. Finally, the innovative combination of whole-genome sequencing and MALDI-TOF MS could be soon the future for diagnosis of antimicrobial resistance in foodborne pathogens.

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“…The original genus consisted of Elizabethkingia meningoseptica and Elizabethkingia miricola. E. meningoseptica is presumably the most recognized species causing hospital-acquired infections, including sepsis in immunocompromised patients and neonatal meningitis [ 4 ], whereas E. miricola is increasingly being isolated from human infections similar to those caused by E. meningoseptica , as well as septic arthritis [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Rare Elizabethkingia anophelis meningitis case in a Danish male

et al. 2018

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Introduction. Elizabethkingia anophelis is a Gram-negative, aerobic, non-motile rod belonging to the family Flavobacteriaceae. Over the last 5 years, it has emerged as an opportunistic human pathogen involved in neonatal meningitis and sepsis, as well as nosocomial outbreaks. It has been isolated from the midgut of the Anopheles gambiae mosquito, but there is no evidence for a role of the mosquito in human infections, and very little is known regarding the routes of transmission to humans. Recent studies, primarily from South-East Asia, suggest that E. anophelis, and not Elizabethkingia meningoseptica, is the predominant human pathogen of this genus. However, identification to the species level has been difficult due to the limitations of the current MALDI-TOF MS (matrix-associated laser desorption ionization-time of flight MS) systems for correct species identification.Case presentation. Here, we present a rare case of E. anophelis meningitis in a Danish male, who had a travel exposure to Malaysia 7 weeks before hospitalization. A multidrug-resistant Elizabethkingia species was isolated from blood and cerebrospinal fluid, and genomic sequencing was used to characterize the phylogenetic position of the isolate, which was determined as associated with previously described sublineage 11. The patient was successfully treated with intravenous moxifloxacin and rifampicin for 2 weeks with no major sequelae, but we did not find the source of transmission.Conclusion. All clinical microbiologists should be aware of the present limitations of the MALDI-TOF MS systems for correct species identification, and therefore we recommend the use of genome sequencing for the correct identification at the species and sublineage level.

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Determination ofElizabethkingiaDiversity by MALDI-TOF Mass Spectrometry and Whole-Genome Sequencing

Cited by 30 publications

References 15 publications

Multicentre study evaluating matrix-assisted laser desorption ionization–time of flight mass spectrometry for identification of clinically isolated Elizabethkingia species and analysis of antimicrobial susceptibility

Multicentre study evaluating matrix-assisted laser desorption ionization–time of flight mass spectrometry for identification of clinically isolated Elizabethkingia species and analysis of antimicrobial susceptibility

MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens

Rare Elizabethkingia anophelis meningitis case in a Danish male

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