Determination of <i>Helicobacter pylori</i> Virulence Genes in Gastric Biopsies by PCR

Essawi, Tamer; Hammoudeh, Wail; Sabri, Israr; Sweidan, W.; Farraj, Mohammad

doi:10.1155/2013/606258

Cited by 35 publications

(29 citation statements)

References 22 publications

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“…The prevalence of H. pylori infection can be attributed to the contribution of specific virulence factors that contribute to the pathogenicity of this organism as enlisted in Table 67.2 Essawi et al, 2013). The prevalence of H. pylori infection can be attributed to the contribution of specific virulence factors that contribute to the pathogenicity of this organism as enlisted in Table 67.2 Essawi et al, 2013).…”

Section: Role Of Virulence Factors In H Pylori Pathogenesismentioning

confidence: 99%

“…pylori genome encodes 32 proteins comprising a cytotoxin CagA and T4SS. Essawi et al (2013) conducted a study to detect the existence of H. pylori along with the rate of cagA, vacA, iceA1, and iceA2 virulence genes in biopsy specimens from symptomatic patients by PCR and found that the rate of positive H. pylori was 44%, while iceA2 was the most frequent virulence gene (84.1%). CagA gene is one of the most important virulence factors located in the DNA segment termed as cag pathogenicity island (cagPAI).…”

Section: Role Of Cagamentioning

confidence: 99%

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Amelioration of Helicobacter pylori-Induced PUD by Probiotic Lactic Acid Bacteria

Kaur

2016

Probiotics, Prebiotics, and Synbiotics

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Section: Role Of Virulence Factors In H Pylori Pathogenesismentioning

confidence: 99%

Section: Role Of Cagamentioning

confidence: 99%

Amelioration of Helicobacter pylori-Induced PUD by Probiotic Lactic Acid Bacteria

Kaur

2016

Probiotics, Prebiotics, and Synbiotics

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“…Molecular methods are widely used to diagnose H. pylori infections as well as for analyses of virulence diversity and resistance patterns of these bacterial strains [4,5]. However, the high degree of genomic plasticity among strains of H. pylori caused the complexity of choosing the target genes.…”

Section: Introductionmentioning

confidence: 99%

“…However, the high degree of genomic plasticity among strains of H. pylori caused the complexity of choosing the target genes. Even nucleotide sequences that are highly conserved in various strains of the pathogen, such as urease A (ureA), urease C (glmM) and 16S rRNA, may fail to detect the bacteria [4,6]. Therefore, another virulence genes can be detected as vacA, cagA genes to confirm the presence of virulent H. pylori strains [3,7].…”

Section: Introductionmentioning

confidence: 99%

INVESTIGATION OF Helicobacter pylori VIRULENCE GENOTYPE IN GASTRIC BIOPSIES BY PCR

Omran

2016

Asian J Pharm Clin Res

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Objectives: Helicobacter pylori infections have been associated with the genetic diversity of their virulence factors; the virulence genotypes are valuable as a molecular marker in the diagnosis of patients with bacterial infections. Our main objective was to analyze the frequency and allelic genotype of vacuolating cytotoxin A (vacA) and cytotoxin-associated gene A (cagA) as well as investigate other virulence genes like outer inflammatory protein A gene (oipA) and a gene contact with the epithelium (iceA) of H. pylori. Methods:A total of 75 patients with gastritis and peptic ulcer diseases (PUDs) were selected to investigate H. pylori infections. 75 antrum biopsies were collected from these patients, and then genomic DNA was extracted from biopsies using the genomic DNA kit. Subsequently, the virulence genes of H. pylori were amplified using specific primers for vacA, cagA, cagE, iceA, and oipA by polymerase chain reaction (PCR).Results: A high prevalence of genes cagA (28.6%), vacAs1bm2 (44.68%), iceA2 (30.6%), and oipA (42.9%) was found, while vacA s2m1 and iceA1 genotypes was not found in our study. There was a significant correlation between the presence of cagA and cagE genotypes (p=0.02), suggesting that these two genes almost used together as a cagPAI integrity marker and the predominant cagA EPIYA motif was ABC (~650 bp) belong to Western cagA strains. The presence of the cagA + and vacA + genes was significantly associated with peptic ulceration (p≤0.001 at a level 0.01 and p≤0.044 at a level 0.05, respectively), whereas different iceA2 genotype was no statistically significant with clinical outcome. Patients with PUD more likely to have an oipA gene (61.9%) than those with gastritis (38.1%), p≤0.037, also the presence oipA gene was statistically significant with presence iceA2 and cagA, as well as the presence iceA gene was statistically significant with the presence vacA. Conclusion: Most H. pylori genotypes which associated with peptic ulcer and gastritis were moderate virulent strains, whereas the virulent strain which associated with peptic ulcer belong to Western cagA strains had vacAs1bm2 genotype, oipA and iceA2 genes that rarely induced gastric cancer in the middle region of Iraq.

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“…33 The first gene of pathogenicity identified in H. pylori was cagA. This gene is highly prevalent in strains from different geographic areas.…”

mentioning

confidence: 99%

Genes de patogenicidade de Helicobacter pylori, polimorfismos de citocinas e fatores ambientais afetam o desenvolvimento de doenças gástricas: uma visão geral

Vianna¹,

Silva²,

Ramis³

2016

Rev Epidemiol Control Infect

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Exceto onde especificado diferentemente, a matéria publicada neste periódico é licenciada sob forma de uma licença Creative Commons -Atribuição 4. pylori possui um conjunto de fatores de patogenicidade, tais como cagA, vacA, iceA, babA, para colonizar a mucosa gástrica e estabelecer infecção crônica. Estes fatores bacterianos são agentes essenciais em modular a resposta imune envolvida na iniciação da carcinogênesegástrica. Os fatores genéticos do hospedeiro contribuem para regular a resposta inflamatória e para o agravamento da lesão da mucosa gástrica uma vez que a infecção gástrica por H. pylori induz a produção de várias citocinas pró e anti-inflamatórias no hospedeiro. O papel prejudicial dos fatores ambientais está relacionado com as precárias condições socioeconômicas, com o consumo de sal, com o tabagismo e com o consumo de álcool. Conclusão: Ao decifrar as regras deterministas -se houver -dessa interação entre fatores da bactéria, do hospedeiro e variáveis ambientais, será possível prever, tratar e, finalmente, prevenir graves doenças gastroduodenais. Background and Objectives:Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach of approximately 50% of the world´s human population. This microorganismis the major causal agent of gastritis and is an important risk factor for the development of peptic ulcer disease and gastric carcinoma. The factors that determine these diverse clinical outcomes are subject to continuous investigations and is thought to be determined by interaction of bacterial factors, host immune system and environmental variables. The aim of this review is to provide an overview of these factors that influence susceptibility to severe outcomes of H. pylori

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Determination of Helicobacter pylori Virulence Genes in Gastric Biopsies by PCR

Cited by 35 publications

References 22 publications

Amelioration of Helicobacter pylori-Induced PUD by Probiotic Lactic Acid Bacteria

Amelioration of Helicobacter pylori-Induced PUD by Probiotic Lactic Acid Bacteria

INVESTIGATION OF Helicobacter pylori VIRULENCE GENOTYPE IN GASTRIC BIOPSIES BY PCR

Genes de patogenicidade de Helicobacter pylori, polimorfismos de citocinas e fatores ambientais afetam o desenvolvimento de doenças gástricas: uma visão geral

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