Determination of <i>trans</i>‐resveratrol in wine by micro‐HPLC with fluorescence detection

López, Ricardo; Dugo, Paola; Mondello, Luigi

doi:10.1002/jssc.200600300

Cited by 17 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resveratrol is found in both the trans-and the cis-isomeric forms, although trans-resveratrol is thought to be the biologically active isoform due to steric stability (3). The biological importance of resveratrol first emerged when it was found present in red wine and was found to be used in traditional Chinese and Japanese medicine (4). Many studies have been published to date demonstrating the beneficial effects of resveratrol in cellular systems.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Resveratrol Action in Lung Cancer Cells Using Dual Protein and Microarray Analyses

et al. 2007

View full text Add to dashboard Cite

Resveratrol, a natural phytoestrogen found in red wine and a variety of plants, is reported to have protective effects against lung cancer; however, there is little work directed toward the understanding of the mechanism of its action in this disease. In this study, we used a combination of experimental approaches to understand the biological activity and molecular mechanisms of resveratrol. Microarray gene expression profiling and high-throughput immunoblotting (PowerBlot) methodologies were employed to gain insights into the molecular mechanisms of resveratrol action in human lung cancer cells. In this report, we confirm the up-regulation of p53 and p21 and the induction of apoptosis by the activation of the caspases and the disruption of the mitochondrial membrane complex. We show the arrest of A549 cells in the G 1 phase of cell cycle in the presence of resveratrol and also report alterations in both gene and protein expressions of cyclin A, chk1, CDC27, and Eg5. Furthermore, the results indicated that resveratrol action is mediated via the transforming growth factor-B pathway, particularly through the Smad proteins. Results showed the down-regulation of the Smad activators 2 and 4 and the up-regulation of the repressor Smad 7 as a result of resveratrol treatment. Resveratrol is a potent inhibitor of A549 lung cancer cell growth, and our results suggest that resveratrol may be a promising chemopreventive or chemotherapeutic agent for lung cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Resveratrol Action in Lung Cancer Cells Using Dual Protein and Microarray Analyses

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The pH in many cases is acidic. Furthermore, because elution of phenolic compounds is greatly facilitated by addition of an acidic modifier, various organic additives, such as triethylamine, 21 trifluoroacetic acid (TFA), 34,35 acetic acid, 23,29,30,33,[36][37][38][39][40][41][42][43][44][45][46][47] formic acid 48,49 and phosphoric acid 50 are always used to improve the resolution and peak shape. It was reported that acetic acid can also prevent the ionization of the hydroxyl groups of resveratrol, and thus increase their retention in reversed phase chromatography.…”

Section: Hplcmentioning

confidence: 99%

Current separative strategies used for resveratrol determination from natural sources

Fan

Lin

et al. 2011

Anal. Methods

View full text Add to dashboard Cite

“…Separation has been performed in most cases with C18 reverse phase columns or, to shorten the separation methodology, coupled monolithic columns (Chromolith Performance RP-18e) to obtain sufficient theoretical plate numbers for the separation (Vian et al 2005). For the detection of the compound, diode array (DAD), fluorescence (FD), and mass spectrometry have been commonly used (Poussier et al 2003;Vian et al 2005;Gürbüz et al 2007;Lopez et al 2007;Rodríguez-Bernaldo de Quirós et al 2007). …”

Section: Introductionmentioning

confidence: 99%

A Powerful Analytical Strategy Based on QuEChERS-Dispersive Solid-Phase Extraction Combined with Ultrahigh Pressure Liquid Chromatography for Evaluating the Effect of Elicitors on Biosynthesis of trans-Resveratrol in Grapes

et al. 2015

View full text Add to dashboard Cite

A powerful methodological approach based on modified quick, easy, cheap effective, rugged, and safe (QuEChERS) extraction technique, followed by cleanup dispersive solid-phase extraction (dSPE) and combined with ultrahigh pressure liquid chromatography (UHPLC), is presented in this paper, for the rapid determination of the healthpromoting phytoalexin, trans-resveratrol, in grapes. This is the first time, to our knowledge, that the combination QuEChERS-dSPE/UHPLC-PDA has been used for trans-resveratrol quantification in grapes. The method has been validated according to European Union Decision 2002/657/EC, in terms of selectivity, linearity, sensitivity, instrument LOD and method LOQ, accuracy, precision, extraction efficiency, and interference assessment. Validation experiments revealed sufficient linearity (R 2 =0.9931) within the established concentration range confirmed by Mandel's fitting test. The sensitivity was good with method detection limit (LOD) of 0.003 μg/mL and quantification limit (LOQ) of 0.010 μg/mL. Optimum QuEChERS-dSPE/UHPLC-PDA conditions led to recoveries of the target analyte in grape samples ranging between 75.1 and 99.7 % and precisions in the 0.9-4 % range (RSD, n=18). The method also afforded satisfactory results in terms of extraction efficiency and interference assessment and provides a valuable and promising tool for determination of trans-resveratrol in grapes with a high resolution within 8 min and a total analysis time of 11 min. The validated methodology was applied to evaluate the effect of the use of elicitors, namely benzothiadiazole and methyl jasmonate, in trans-resveratrol biosynthesis on Vitis vinifera Monastrell grapes. The results obtained revealed an increase of trans-resveratrol level of 2-fold for grapes treated with benzothiadiazole and 3.5-fold for grapes treated with methyl jasmonate.

show abstract

Determination of trans‐resveratrol in wine by micro‐HPLC with fluorescence detection

Cited by 17 publications

References 36 publications

Molecular Mechanisms of Resveratrol Action in Lung Cancer Cells Using Dual Protein and Microarray Analyses

Molecular Mechanisms of Resveratrol Action in Lung Cancer Cells Using Dual Protein and Microarray Analyses

Current separative strategies used for resveratrol determination from natural sources

A Powerful Analytical Strategy Based on QuEChERS-Dispersive Solid-Phase Extraction Combined with Ultrahigh Pressure Liquid Chromatography for Evaluating the Effect of Elicitors on Biosynthesis of trans-Resveratrol in Grapes

Contact Info

Product

Resources

About