Determination of β‐N‐oxalyl‐<scp>l</scp>‐α,β‐diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis

Cristóbal, Mara Sacristán San; Varela, Alejandro; Pedrosa, Mercedes M.; Burbano, Carmen; Cuadrado, Carmen; Legaz, Marı́a Estrella; Múzquiz, M.

doi:10.1002/jsfa.6792

Cited by 19 publications

(14 citation statements)

References 32 publications

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“…The causative agent for lathyrism is the non-protein amino acid β-ODAP, also referred to as β-N-oxalylamino-L-alanine (BOAA). This compound was analyzed in a previous work using a CZE method (Sacristán et al, 2015). In L. cicera seeds, samples ZL41 and ZL02, the β-ODAP content (data not shown in table 4, in Sacristán et al, 2015 ) was similar (0.79 and 0.80 mg/g, respectively) and below the recommended toxic values for nutrition (Anadon-Navarro et al, 2010).…”

Section: β-N-oxalyl-l-α β-Diaminopropionic Acid (β-Odap)mentioning

confidence: 90%

“…This compound was analyzed in a previous work using a CZE method (Sacristán et al, 2015). In L. cicera seeds, samples ZL41 and ZL02, the β-ODAP content (data not shown in table 4, in Sacristán et al, 2015 ) was similar (0.79 and 0.80 mg/g, respectively) and below the recommended toxic values for nutrition (Anadon-Navarro et al, 2010). Taking into account the average dietary inclusion level of L. cicera in feed formulations (usually as a partial substitute of soy) and the low ODAP levels here found, L. cicera seeds (ZL41 and ZL02) might be used safely in feed formulations as a potential substitute of soy.…”

Section: β-N-oxalyl-l-α β-Diaminopropionic Acid (β-Odap)mentioning

confidence: 90%

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Biochemical characterization of legume seeds as ingredients in animal feed

Martín-Pedrosa¹,

Varela²,

Guillamón³

et al. 2016

Span. j. agric. res.

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competition of feedstock proteins produced mainly in North and South America; the increased use of chemical fertilizers in crop production; the availability of cheap sources of animal protein (fish and meat-beef, pork and poultry), and the simplification of agricultural systems. In fact, during the last decades the cultivation of grain legumes in Europe, as well as their use, fell sharply: in 2010 the total production of pulses in Europe was 39% of that obtained in 1990 (FAO Stat: http://faostat3.fao.org/home/index.html). As a consequence, Europe presently needs to import most of the RESEARCH ARTICLE OPEN ACCESS AbstractThe current European protein deficit is estimated as high as 70% of present needs. Because of the high protein content of their seeds, grain legumes are attractive candidates for lowering the deficiency in plant protein production. The objective of this work was to identify new sources of vegetable protein that would reduce our high dependence of soy, the main source of protein in the manufacture of feedstuffs. To achieve this goal, we determined the proximate composition, the bioactive components, as well as the antinutritional factors present in the studied seeds. In general, the protein, fat and carbohydrates content of legume seeds studied were within the limits found in the literature. The bioactive compounds detected in all the seeds were α-galactosides, myoinositol phosphates, protease inhibitors and phenols. IP 6 (phytic acid) was the main inositol phosphate form in all the samples. The highest protease inhibitors content was detected in both Lathyrus cicera cultivars. Vicia ervilia and L. cicera cultivars showed low haemagglutinating activity (20.4 HU/g). The γ-glutamyl-S-ethenyl-cysteine content in Vicia narbonensis was around 16.0 mg/g. Both L. cicera varieties presented similar β-Noxalyl-L-α, β-diaminopropionic acid content (0.80 mg/g). The two V. ervilia varieties showed high canavanine concentration (1.93-5.28 mg/g). Vicine was only detected in V. narbonensis cultivars (0.3 mg/g). The biochemical characterization carried out in this study allows us to know the limits of inclusion of these minor crop seeds in feed formulations in order to replace the soybean.Additional key words: legumes; bioactive compounds; feedstuffs; Cicer; Vicia; Lathyrus Abbreviations used: ANF (anti-nutritional factors); CIU (chymotrypsin inhibitor unit); GEC (γ-glutamyl-S-ethenyl-cysteine); HU (haemagglutinating unit); IP (inositol phosphates); ORAC (oxygen-radical absorbance capacity); PCAF (sodium amminepentacyanoferrate (II) hydrat); SBM (soybean meal); SCFA (short-chain fatty acids); TIA (trypsin inhibitor activity); TIU (trypsin inhibitor unit); β-ODAP (β-N-oxalyl-L-α, β-diaminopropionic acid)Citation: Martín-Pedrosa, M.; Varela, A.; Guillamon, E.; Cabellos, B.; Burbano, C.; Gomez-Fernandez, J.; de Mercado, E.; Gomez-Izquierdo, E.; Cuadrado, C.; Muzquiz, M. (2016). Biochemical characterization of legume seeds as ingredients in animal feed.

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Section: β-N-oxalyl-l-α β-Diaminopropionic Acid (β-Odap)mentioning

confidence: 90%

Section: β-N-oxalyl-l-α β-Diaminopropionic Acid (β-Odap)mentioning

confidence: 90%

Biochemical characterization of legume seeds as ingredients in animal feed

Martín-Pedrosa¹,

Varela²,

Guillamón³

et al. 2016

Span. j. agric. res.

Self Cite

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“…Basaran et al [31] determined that the β-ODAP content of 52 grass pea populations grown under Samsun conditions in Turkey was 1.40 mg/g and 3.05 mg/g. In their study on the Lathyrus species, Sacristan et al [32] determined that the β-ODAP content was between 0.79 mg/g and 5.05 mg/g, and the homoarginine content was between 7.49 and 12.44 mg/g. Zhao et al [33] determined that the β-ODAP content in grass pea seeds was between 0.16% and 0.58%, while the homoarginine content was between 0.32% and 1.06%.…”

Section: Calibration Curvesmentioning

confidence: 99%

β-N-oxalyl-L-2,3-diaminopropionic acid, L-homoarginine, and asparagine contents in the seeds of different genotypes Lathyrus sativus L. as determined by UHPLC-MS/MS

Arslan

Öten²,

Erkaymaz

et al. 2017

International Journal of Food Properties

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Lathyrus sativus L. is considered one of the most promising calorie and protein sources for the vast, expanding populations living in drought-prone and marginalized areas of the world. This study was conducted to determine the β-N-oxalyl-L-2,3-diaminopropionic acid (β-ODAP), L-homoarginine, and asparagine content of a total of 173 Lathyrus sativus L. genotypes, of which 93 were collected under natural conditions in Antalya. The β-ODAP, L-homoarginine, and asparagine content of Lathyrus sativus L. genotypes ranged from 1.55 to 20.8 mg/g seeds, 1.35 to 11.64 mg/g seeds, and 0.59 to 5.22 mg/g seeds, respectively. ARTICLE HISTORY

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“…This is due to the non‐protein AA β‐N‐oxalyl‐1‐a,b‐diaminopropionic acid. Thus, its amount was determined in L. sativus and L. cicera by CE . There is also particular interest in homoarginine levels in human and animal nutrition.…”

Section: Vegetables and Fruitsmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

et al. 2017

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Recent advances in amino acid analysis by capillary electromigration methods: June 2015-May 2017In the tenth edition of this article focused on recent advances in amino acid analysis using capillary electrophoresis, we describe the most important research articles published on this topic during the period from June 2015 to May 2017. This article follows the format of the previous articles published in Electrophoresis. The new developments in amino acid analysis with CE mainly describe improvements in CE associated with mass spectrometry. Focusing on applications, we mostly describe clinical works, although metabolomics studies are also very important. Finally, works focusing on amino acids in food and agricultural applications are also described.

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Determination of β‐N‐oxalyl‐l‐α,β‐diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis

Cited by 19 publications

References 32 publications

Biochemical characterization of legume seeds as ingredients in animal feed

Biochemical characterization of legume seeds as ingredients in animal feed

β-N-oxalyl-L-2,3-diaminopropionic acid, L-homoarginine, and asparagine contents in the seeds of different genotypes Lathyrus sativus L. as determined by UHPLC-MS/MS

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

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