Determination of Ibuprofen and Paracetamol in Binary Mixture Using Chemometric-Assisted Spectrophotometric Methods

Abstract

Three methods are presented for simultaneous determination of ibuprofen and paracetamol without previous separation. The first method depends on first derivative UV spectrophotometry, with zero-crossing measurement. The first derivative amplitudes at 230 and 290 nm were selected for the assay of ibuprofen and paracetamol, respectively. The second method depends on first derivative of the ratio-spectra by measurements of the amplitudes at 280 and 290 nm for ibuprofen and paracetamol, respectively. Calibration graphs were established in the range of 5-100 and 10-100 µg mL^-1 for ibuprofen and paracetamol, respectively. The third method is the use of multivariate spectrophotometric calibration for the simultaneous determination of the analyzed mixture. The resolution of the studied binary mixture has been accomplished by using partial least squares (PLS) regression analysis. Although the components show an important degree of spectral overlap, they have been simultaneously determined with high accuracy, with no interference from pharmaceutical dosage form excipients. A comparison is presented with the related multivariate method of classical least squares (CLS) analysis, which is shown to yield less reliable results due to severe spectra overlap presented by the studied compounds. All of the proposed methods have been extensively validated. These methods allow a number of cost and time saving benefits. The described methods can be readily utilized for analysis of pharmaceutical formulations. There was no significant difference between the performance of all of the proposed methods regarding the mean values and standard deviations

Simultaneous Determination of Acetaminophen and Synthetic Color(s) by Derivative Spectroscopy in Syrup Formulations and Validation by HPLC: Exposure Risk of Colors to Children

“…The differential derivative method showed recovery of all the components in the range of 95.2-103.1%. The values indicate an adequate recovery rate, and the recovery percentages found in the present study are in close resemblance to other methods (22,44,46,47). …”

“…Literature survey reveals various analytical techniques, with chromatographic methods playing a significant role in pharmaceutical analysis (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). However, these methods require sophisticated and expensive equipment, provision for use and disposal of solvents, labor-intensive sample preparation procedures, and personal skills.…”

Properties of ibuprofen ion-selective electrodes based on the ion pair complex of tetraoctylammonium cation

“…Table 7 presents a comparison of the results obtained for the proposed method with the reported analytical methods. The working range offered by the presented electrode (22.83-22829 mg mL -1 ) is compared with spectrophotometry (5-3200 mg mL -1 ) [15][16][17] and potentiometric titration (1-10 mg mL -1 ) [24]. The accuracy and precision of the potentiometric method (the mean recovery 99.5-105.2%) is much better than the HPLC method (78-95%) [22] and it is comparable with the accuracy of other methods (capillary electrophoresis 97.65-104.66% [25] spectrofluorimetry 97.9-105% [18,19]).…”

“…The various methods include the use of spectrophotometry [15][16][17], spectrofluorimetry [18][19], high-performance liquid chromatography (HPLC) with UV detection [20], HPLC-ELSD (evaporative light scattering detection) [21], HPLC [22], potentiometric titration [23,24], conductometric titration [24], capillary electrophoresis [25], and luminescence [26].…”

Novel Ratio Subtraction and Isoabsorptive Point Methods for Determination of Ambroxol Hydrochloride and Doxycycline in their Combined Dosage Form: Development and Validation

“…Several spectrophotometric methods were used for resolving such mixtures with overlapping spectra. Derivative spectrophotometry [1], dual wave length spectrophotometry [2], pH-induced differential spectrophotometry [3] and chemometric methods [4] were all used for such , is an active metabolite of bromhexine. It is used a mucolytic agent used to treat respiratory disorders associated with viscid or excessive mucus [5].…”