Determination of IgE antibodies to the benzyl penicilloyl determinant. A comparison between poly-l-lysine and human serum albumin as carriers

Blanca, Miguel; Mayorga, Cristobalina; Pérez, E.; Suau, Rafael; Juárez, C.; Vega, José M.; Carmona, Marı́a José; Perez-Estrada, M.; Garcı́a, J.J.

doi:10.1016/0022-1759(92)90311-g

Cited by 81 publications

(66 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These were made by RAST to PG, AX, AMP and to different cephalosporins (ceftazidime, ceftriaxone, cefuroxime, cefotaxime, cefadroxile, cephalexine) conjugated to poly-Llysine (PLL) (Sigma) as previously described (Blanca et al, 1992). Blood samples were obtained when patients were evaluated and sera were kept at À20°C until assayed.…”

Section: Immunochemical Studiesmentioning

confidence: 99%

Synthesis, characterization and immunochemical evaluation of cephalosporin antigenic determinants

Sánchez-Sancho

Pérez‐Inestrosa

Suau

et al. 2003

J of Molecular Recognition

View full text Add to dashboard Cite

Lack of knowledge of the exact chemical structure of cephalosporin antigenic determinants has hindered clinical interpretation of adverse reactions to these drugs and delayed understanding of the mechanisms involved in the specific recognition and binding of IgE molecules to these antigenic determinants. We further resolve the relationship between structure and activity of proposed antigenic chemicals, including the rational design and synthesis of these haptenic structures. Comparative RAST inhibition studies of the synthesized molecules revealed that they were recognized by IgE antibodies induced by cephalosporin antibiotics. Thus, these data indicate that recognition is mainly directed to the acyl side chain and to the beta-lactam fragment that remains linked to the carrier protein in the cephalosporin conjugation course.

show abstract

Section: Immunochemical Studiesmentioning

confidence: 99%

Synthesis, characterization and immunochemical evaluation of cephalosporin antigenic determinants

Sánchez-Sancho

Pérez‐Inestrosa

Suau

et al. 2003

J of Molecular Recognition

View full text Add to dashboard Cite

show abstract

“…Solid-phase discs were prepared following a modification of the method o f Edwards et al [14], Polylysine (10 kD molecular weight, Sigma Chemical Co, lOmg/ml) was coupled to cyanogcn-bromideactivated cellulose discs and then treated with BP or AX as previously described [15]. Control discs o f polylysine only were also prepared.…”

Section: Rast Discsmentioning

confidence: 99%

“…Inhibition studies were carried out as described [15]. All inhibitors were dissolved in a mixture o f PBS (88%) and 1 A sodium hydroxide (12%) prior to use.…”

Section: Rast Inhibitionmentioning

confidence: 99%

Studies of the Specificities of IgE Antibodies Found in Sera from Subjects with Allergic Reactions to Penicillins

Moreno¹,

Blanca²,

Mayorga³

et al. 1995

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Penicillins are immunogenic when administered to humans and in some instances they can also be allergenic, inducing specific IgE antibodies. Whilst the major haptenic group, the penicilloyl, is well characterised, less is known about the relative importance of the different parts of the structure for antibody binding and how this can influence the specificity of patients response. In order to investigate this further, sera from subjects who had suffered an IgE-mediated reaction to penicillins were studied using the radioallergosorbent test (RAST) and RAST inhibition. The assays employed reagents related to the penicillins causing the reaction. Using 173 sera, positive RAST results were only found with reagents based on benzyl penicillin (BP) and amoxicillin (AX). Fifty-three positive sera were selected for further studies and categorized into three groups: (A) sera only RAST positive to AX, (B) sera only positive to BP and (C) sera positive to both penicillins. RAST inhibition studies were then carried out using monomeric penicilloyl conjugates and compounds representing parts of the penicilloyl structures of BP and AX. For all three groups, monomeric penicilloyl conjugates were the most efficient inhibitors but there were differences for the other compounds. Group A sera were also inhibited by the side chain of amoxicillin, whereas group B sera were poorly inhibited by all other inhibitors. Group C sera showed two patterns of inhibition, both consistent with their more cross-reactive profile. Some group C sera were similar to group B, showing inhibition with monomeric penicilloyl conjugates only, whilst others were similar to group A showing inhibition with side-chain-related compounds, although in these cases all the side chain compounds inhibited all the assays. We conclude that in sera from patients allergic to penicillins, IgE antibodies of different specificities can be found reflecting the involvement of the penicillins in inducing the allergic reaction. Detailed inhibition analysis indicates that two main groups of antibodies can be distinguished: one where the side chain contributes a unique specificity to the antigen binding site and in which they are positive to AX, and another where most of the structure is required for optimal inhibition. However, a considerable variation in the pattern of recognition of the antigenic determinant was seen. No coexisting antibodies of different specificities were detected in the same patient.

show abstract

“…These include red blood cells (12), polyacrylamide particles (13), transferrin (14), polylysine (7), epoxyactivated resins (15), an aminospacer (SP) (16), and direct conjugation of BP to a polystyrene solid phase (17). Both radioimmunoassay (7,9,12,15,16,(18)(19)(20) and enzyme-linked immunoassay (11,14,17,(21)(22)(23)) methods have been used. A few comparative studies of in vitro methods using either antibodies from experimental animals (24) or human antibodies (12,16,25) have been reported, but no systematic attempts to determine the sensitivity and specificity of the assays have been carried out.…”

Section: Introductionmentioning

confidence: 99%

Determination of IgE antibodies to the benzylpenicilloyl determinant: A comparison of the sensitivity and specificity of three radio allergo sorbent test methods

Garcı́a¹,

Blanca²,

Moreno³

et al. 1997

J. Clin. Lab. Anal.

View full text Add to dashboard Cite

Determination of IgE antibodies to the benzyl penicilloyl determinant. A comparison between poly-l-lysine and human serum albumin as carriers

Cited by 81 publications

References 21 publications

Synthesis, characterization and immunochemical evaluation of cephalosporin antigenic determinants

Synthesis, characterization and immunochemical evaluation of cephalosporin antigenic determinants

Studies of the Specificities of IgE Antibodies Found in Sera from Subjects with Allergic Reactions to Penicillins

Determination of IgE antibodies to the benzylpenicilloyl determinant: A comparison of the sensitivity and specificity of three radio allergo sorbent test methods

Contact Info

Product

Resources

About