Determination of IgM and IgG antibodies to Toxoplasma using the IFA test, ELISA, and Dot-ELISA procedures

Pappas, Michael G.; Lunde, Milford N.; Hajkowski, Ruta; McMahon, James

doi:10.1016/0304-4017(86)90090-7

Cited by 17 publications

(11 citation statements)

References 24 publications

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“…Our data show that standardization with the saline extract antigen showed the best results in the Dot-ELISA, allowing a clear distinction between positive and negative samples. These results are supported by data reported by other authors [1,18] who used the Dot-ELISA with saline for antigen detection of IgG anti-T. gondii in serum samples. Our results were also similar to those described by Morris et al (2002) in studies on detecting anti- T. gondii in saliva, [9,14] We also confirmed the presence of IgG in saliva in our assay.…”

Section: Discussionsupporting

confidence: 90%

“…The main tool used for the diagnosis of toxoplasmosis is serology, and several serum tests were recommended for the diagnosis of toxoplasmosis [4]. The Dot-ELISA has been described as an efficient diagnostic method for infectious parasitic diseases such as Cutaneous Leishmaniasis [25], schistosomiasis [21], toxoplasmosis [18],and angiostrongyliasis [6].…”

Section: Discussionmentioning

confidence: 99%

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Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana

Sampaio¹

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana

Sampaio¹

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“…A modification of IFA (IgM-IFA) was developed by Remington (1969) to detect immunoglobulin M (IgM) antibodies in congenitally infected children and could detect 75% of congenital infections. Pappas et al (1986) used 1.5% formalin fixed tachyzoites of T. gondii (RH strain) as antigen in IFAT and found four out of 56 humans sera positive for IgM and 51 out of 56 (91%) for IgG antibodies at a reciprocal titre of ≥16. In United States, a manual of the Department of Health, Education and Welfare (USDHEW) provided a procedural guide for the performance of IFAT in detecting T. gondii antibodies.…”

Section: Indirect Immune Fluorescent Testmentioning

confidence: 99%

Recent trends in the diagnosis of toxoplasmosis

Sudan¹

2013

Clin. Rev. Opinions

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Toxoplasma gondii is one of the most widely prevalent cyst forming apicomplexan parasite present worldwide. Felines serve as definitive hosts, while all non-feline vertebrates including humans, act as intermediate hosts of the parasite. There are several serological tests available for the detection of T. gondii antibodies such as the dye test (DT), indirect haemagglutination test (IHA), complement fixation test (CFT), modified agglutination test (MAT), latex agglutination test (LAT), indirect immunofluorescent test (IFAT) and direct agglutination test (DAT). The dye test once considered as gold standard is seldom used nowadays, owing to potent hazardous effects because of involvement of live tachyzoites. Of late, enzyme linked immune sorbent assays (ELISA) have gained a lot of utility in diagnosing toxoplasmosis.Other than serological tests, bioassays, polymerase chain reaction (PCR) based assays and histopathologies are also used. The various tests used for diagnosis of toxoplasmosis, along with various molecules being employed, coupled with their advantages and limitations, are described and discussed in detail in the present review.

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“…Anti-T. gondii serum, moreover, were produced by immunizing rabbits. Prior to inoculation, all the donors were confirmed as N. caninum and T. gondii negative by IFAT (15,16). To obtain high immune response similar with natural infection, a cow was intravenously inoculated with 1 ϫ 10 8 live tachyzoites of the KBA-2 isolate suspended in phosphate-buffered saline (PBS).…”

Section: Preparation Of Anti-n Caninum and Anti-t Gondii Serum Frommentioning

confidence: 99%

Development of competitive ELISA for neosporosis by employing immunoproteomics

et al. 2004

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In this study, proteomics was used to explore the antigenic proteins that are involved in crossreactivity during serodiagnosis between Neospora caninum (N. caninum) and Toxoplasma gondii (T. gondii). Competitive enzyme-linked immunosorbent assay (C-ELISA) developed by proteomics shed a new light on the infection of N. caninum. Cross-reactivity of antigenic proteins between N. caninum and T. gondii tachyzoites was explored by using the conventional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) (1-DE) and two-dimensional gel electrophoresis (2-DE) immunoblot. The proteins were identified by matrix-assisted laser desorption/ionizationtime of flight mass spectrometry. The protein expression patterns in the immunoblot profiles of N. caninum were similar to bovine, chicken, and rabbit anti-N. caninum serum, but they were not similar to rabbit anti-T. gondii serum. Band at 79 kDa, HSP70, and actin on immunoblot profiles

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Determination of IgM and IgG antibodies to Toxoplasma using the IFA test, ELISA, and Dot-ELISA procedures

Cited by 17 publications

References 24 publications

Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana

Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana

Recent trends in the diagnosis of toxoplasmosis

Development of competitive ELISA for neosporosis by employing immunoproteomics

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