Determination of IL‐1 and IL‐6 Levels in Human Embryo Culture‐Conditioned Media

Barañao, Rosa Inés; Piazza, Alejandra; Rumi, L; Fried, Ester Polak de

doi:10.1111/j.1600-0897.1997.tb00212.x

Cited by 55 publications

(36 citation statements)

References 17 publications

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“…Results in this study indicate that CM prepared with bovine cumulus cells, either serum-containing CM, or BSA-containing CM, yielded similar morula and blastocyst development compared with co-culture with bovine cumulus cells (Table 3). There have, however, been numerous studies reporting the incubation of in vitro produced (IVP) or in vivo embryos in medium conditioned with different cells (Kobayashi et al 1992, 1997, Baranao et al 1997, Liu et al 1998, Joo et al 2001. Cell numbers of blastocysts derived in CM were not different from coculture groups.…”

Section: Discussionmentioning

confidence: 99%

“…Embryos have also been reported to secrete substances into their surrounding environment (Van Langendoncket et al 1996, Izquierdo et al 1999. Cell-free conditioned medium (CM) has been used in several laboratories in the culture of in vivo and in vitro fertilized embryos (Baranao et al 1997, Funston et al 1997, Ishiwata et al 2000, Galli et al 2003. Studies have shown that CM has similar effects on embryo development to those produced in various co-culture systems.…”

Section: Introductionmentioning

confidence: 98%

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Conditioned medium increases the polyploid cell composition of bovine somatic cell nuclear-transferred blastocysts

White

Aston

et al. 2004

Reproduction

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The effects of bovine cumulus cell-conditioned medium on cloned bovine embryonic development and subsequent chromosome complement were examined using an air-dry procedure. Conditioned media were prepared using CR1aa supplemented with either fetal bovine serum (FBS) or bovine serum albumin (BSA). Nuclear-transferred embryos were reconstructed with nuclei from cumulus cells. Similar cleavage, morula, and blastocyst development was observed in conditioned media groups compared with the co-culture group. No differences (P > 0.05) were observed in the composition of blastocyst chromosomes after co-culture in different media, either with or without starvation of donor cells. The overall diploid blastocyst rate ranged from 75% to 84%. Chromosomal complement of blastocysts, however, was very different between conditioned medium and co-culture treatments. Overall incidence of chromosomal anomalies was 40% in conditioned medium, which was significantly higher (P < 0.001) than the co-culture group (20%). Moreover, a higher incidence (P < 0.05) of chromosomally abnormal blastocysts (41.5%) was observed after culture with FBS-containing conditioned medium than those cultured in BSA-containing conditioned medium (31.4%). No diploid improvement was observed after exchange of the culture system from conditioned medium to co-culture, or from co-culture to conditioned medium after the first 72 h of culture. The results of this study also indicated that the overall cell number was much lower (P < 0.01) in blastocysts with chromosomal abnormalities than those with a normal diploid state. We have concluded that medium conditioned with bovine cumulus cells increases the incidence of chromosomal anomalies in nuclear reconstructed embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Conditioned medium increases the polyploid cell composition of bovine somatic cell nuclear-transferred blastocysts

White

Aston

et al. 2004

Reproduction

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“…Preimplantation embryos are known to produce a number of cytokines including interleukin-1a (Sheth et al, 1991), interleukin-1b (Takacs and Kauma, 1996;Krussel et al, 1998) and interleukin-6 (Zolti et al, 1991;Gerwin et al, 1995). In fact, the production of interleukin-1 had been suggested to be a marker of embryo quality in clinical assisted reproduction (Baranao et al, 1997). Functional response elements of interleukin-1 and interleukin-6 are present in the mouse C3 gene (Kawamura et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Regulation of complement‐3 protein expression in human and mouse oviducts

Lee

Cheong

Chow

et al. 2008

Molecular Reproduction Devel

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SUMMARYThe human oviduct derived embryotrophic factor-3 (ETF-3) contains complement protein-3 (C3) and its derivates. Although C3 is not embryotrophic, it is converted into the embryotrophic derivative, iC3b in the presence of embryos and oviductal cells. The regulation of C3 production in the oviduct is not known. The objectives of this study were to investigate the effects of presence of preimplantation embryos and hormones on C3 expression in the oviducts in vitro and in vivo. The expression of C3 in the oviduct of pregnant mice was compared to that of pseudo-pregnant mice. The hormonal action on C3 expression was studied in the ovariectomized mouse oviducts and human oviductal epithelial (OE) cells. The results showed that the level of C3 mRNA in the mouse oviduct was high on day 1 and Day 2, but decreased to a minimum on day 4 of pregnancy, whereas that of pseudo-pregnancy remained relatively stable within the same period. The protein levels of C3 and iC3b specific fragments, a-115 and a-40, respectively in the mouse oviductal luminal fluid were highest on day 3 of pregnancy, when the embryos were expected to be most sensitive to the embryotrophic activity of ETF-3. Estrogen elevated C3 expression in the ovariectomized mouse oviduct and the OE cells. Progesterone suppressed estrogeninduced C3 expression in the mouse oviduct, but had no effect on OE cells. In conclusion, the presence of embryo and steroid hormones regulate the synthesis and secretion of oviductal C3.

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“…The embryo produces several factors during its development to signal its presence to the maternal organism, and several embryonic factors that modulate endometrial receptivity have been reportedly detected in embryo culture supernatant (ECS) (2)(3)(4)(5)(6)(7)(8). In blastocyst transfer (BT), cross-talk is absent until the blastocysts are transferred and the pregnancy rate (PR) using BT is limited to around 40% (9).…”

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confidence: 99%