Determination of illegal antimicrobials in aquaculture feed and fish: An ELISA study

Conti, Gea Oliveri; Copat, Chiara; Wang, Zhanhui; D’Agati, Placido; Cristaldi, Antonio; Ferrante, Margherita

doi:10.1016/j.foodcont.2014.10.050

Cited by 74 publications

(10 citation statements)

References 28 publications

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“…Although these drugs are highly effective against fish diseases caused by fungal and bacterial infections in aquatic environments, they are banned in aquaculture practices because they may pose health risks and adverse environmental impact [13]. However, these prohibited drugs are still frequently used in many places around the world, due to their low cost and effectiveness in the control of fish diseases [14,15,16].…”

Section: Introductionmentioning

confidence: 99%

Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

et al. 2016

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Surface-enhanced Raman scattering or surface-enhanced Raman spectroscopy (SERS) is a promising detection technology, and has captured increasing attention. Silver nanowires were synthesized using a rapid polyol method and optimized through adjustment of the molar ratio of poly(vinyl pyrrolidone) and silver nitrate in a glycerol system. Ultraviolet-visible spectrometry, X-ray diffraction, and transmission electron microscopy were used to characterize the silver nanowires. The optimal silver nanowires were used as a SERS substrate to detect prohibited fish drugs, including malachite green, crystal violet, furazolidone, and chloramphenicol. The SERS spectra of crystal violet could be clearly identified at concentrations as low as 0.01 ng/mL. The minimum detectable concentration for malachite green was 0.05 ng/mL, and for both furazolidone and chloramphenicol were 0.1 μg/mL. The results showed that the as-prepared Ag nanowires SERS substrate exhibits high sensitivity and activity.

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Section: Introductionmentioning

confidence: 99%

Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

et al. 2016

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“…These fish drugs have been widely used in aquatic settings owing to the effective antibacterial property, high effectivity, and low cost. However, it is well-noticed that these drugs are harmful to human health and rigidly restricted by FDA and many other organizations . Therefore, it is important to detect the residues of these prohibited fishery drugs rapidly and accurately.…”

Section: Resultsmentioning

confidence: 99%

Au/Ag Bimetallic Nanocuboid Superlattices Coated with Ti₃C₂ Nanosheets for Surface-Enhanced Raman Spectroscopy Detection of Fish Drug Residues in Pond Water

Miao

Kuo

Zong

et al. 2021

ACS Appl. Nano Mater.

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Applying nanomaterials to highly sensitive, fast, and accurate detection of hazardous chemicals in water remains to be a great challenge. Herein, a Ti 3 C 2 -functionalized superlattice SERS substrate was developed, which can identify multiplex fish drug residues in pond water with a high sensitivity and reliable reproducibility. Specifically, a superlattice substrate was fabricated by vertically self-assembled bimetallic nanocuboids (BNCs) through the Marangoni effect, which exhibited an excellent SERS activity. Furthermore, the modification of the Ti 3 C 2 layer endows the substrate with both an increased SERS signal and an enhanced adsorption for multiple fish drugs, including crystal violet (CV), malachite green (MG), and methylene blue (MB). The limit of detection (LOD) was as low as 10 −12 M, while the signal fluctuation was as small as ∼5.9% between batches. More importantly, a trace amount of multiplex fish drug residues was detected in natural pond water, demonstrating a convincing practicality of such a Ti 3 C 2 -functionalized superlattice SERS substrate.

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“…[1,2,3,4] Conventional ELISA sensors can detect hazardous chemicals in foods environment and are used in biomedical diagnosis and chemical quality controls. [5,6,7] However, certain limitations of the current ELISA sensing materials exist, including relatively high cost, lack of scalability and flexibility for personal use, and simultaneous examinations of multitarget chemicals in very low concentrations, as well as dependence on specialized instrumentation. [8] As an alternative, paper-based ELISA (p-ELISA) uses fibrous and microporous platforms with advantages of increased surface areas of fibers, reduced cost, ease of use, and improved detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Naked Eye Detectable Colorimetric Biosensors Made from Macroporous Framework Melamine Foams for Onsite and Simultaneous Detection of Multiple Environmental Hazards in Flowing Through Sensing Systems

Pan,

Zhao,

Norwood

et al. 2023

Advanced Sensor Research

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Personal‐use, naked eye‐readable, low‐cost, highly sensitive, and selective biosensors for rapid detection of environmental toxicants are relevant for many application scenarios. Here, the recent developments of highly sensitive and naked eye distinguishable colorimetric sensors by using commercially available melamine foam (MF) as basic sensing materials for instant and volume‐responsive simultaneous detection of multiple targets in fluid systems are reported. The MF possesses a unique reticulated three‐dimensional (3D) macroporous framework structure enabling rapid mass transfer of large biomolecules through the structures in all directions, ensuring easy access of numerous active binding sites of the chemically modified framework to the proteins and target molecules, and subsequently providing significantly increased sensitive and volume‐responsive detection of target molecules in flowing through sensor systems. Promising results in direct, sandwich, and competitive ELISA tests demonstrated the great application potential of the materials. Besides, an additive and simultaneous detection of two targets in one system is achieved by using different layers of the sensor materials in a flowing‐through filtering device. The novel biosensors are expected to significantly improve the sensitivity and broaden the applications of ELISA in the rapid detections of trace amounts of toxicants in liquid and aerosol systems.

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Determination of illegal antimicrobials in aquaculture feed and fish: An ELISA study

Cited by 74 publications

References 28 publications

Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

Au/Ag Bimetallic Nanocuboid Superlattices Coated with Ti₃C₂ Nanosheets for Surface-Enhanced Raman Spectroscopy Detection of Fish Drug Residues in Pond Water

Highly Sensitive Naked Eye Detectable Colorimetric Biosensors Made from Macroporous Framework Melamine Foams for Onsite and Simultaneous Detection of Multiple Environmental Hazards in Flowing Through Sensing Systems

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Determination of illegal antimicrobials in aquaculture feed and fish: An ELISA study

Cited by 74 publications

References 28 publications

Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

Au/Ag Bimetallic Nanocuboid Superlattices Coated with Ti3C2 Nanosheets for Surface-Enhanced Raman Spectroscopy Detection of Fish Drug Residues in Pond Water

Highly Sensitive Naked Eye Detectable Colorimetric Biosensors Made from Macroporous Framework Melamine Foams for Onsite and Simultaneous Detection of Multiple Environmental Hazards in Flowing Through Sensing Systems

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Au/Ag Bimetallic Nanocuboid Superlattices Coated with Ti₃C₂ Nanosheets for Surface-Enhanced Raman Spectroscopy Detection of Fish Drug Residues in Pond Water