Determination of illicit and medicinal drugs and their metabolites in oral fluid and preserved oral fluid by liquid chromatography–tandem mass spectrometry

Concheiro, Marta; Castro, Ana de; Quintela, Óscar; Cruz, Angelines; López-Rivadulla, Manuel

doi:10.1007/s00216-008-2135-4

Cited by 94 publications

(68 citation statements)

References 12 publications

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“…The collected volumes of oral fluid were determined for each sample by weight. Drug concentrations in undiluted oral fluid were calculated by considering oral fluid density as 1 g/ml (Concheiro, de Castro, Quintela, Cruz, & Ló pez-Rivadulla, 2008). The analyses were carried out at both times by the University of Santiago de Compostela.…”

Section: Substances Analyzed and Toxicological Aspectsmentioning

confidence: 99%

Alcohol and drug use by Spanish drivers: Comparison of two cross-sectional road-side surveys (2008–9/2013)

Fierro¹,

Luque

Seguí-Gómez

et al. 2015

International Journal of Drug Policy

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Section: Substances Analyzed and Toxicological Aspectsmentioning

confidence: 99%

Alcohol and drug use by Spanish drivers: Comparison of two cross-sectional road-side surveys (2008–9/2013)

Fierro¹,

Luque

Seguí-Gómez

et al. 2015

International Journal of Drug Policy

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“…The automated SPE method is simple and has few steps, allowing for effective processing of large numbers of samples. An LC-MS/MS method with automated SPE that uses a Gilson robot for the extraction was recently reported (8 ). The authors used different SPE columns and solvent for elution than we did, and had a low recovery for benzoylecgonine (7.5%), whereas our method showed 66% recovery for benzoylecgonine.…”

Section: Discussionmentioning

confidence: 76%

“…To fulfill this demand, it is important to have good recoveries for all of the compounds measured, which may be difficult to attain (7,8 ). In the initial development phase of our method, we focused on attaining adequate recoveries for THC, buprenorphine, and morphine, and succeeded in achieving recoveries exceeding 50% for all of the compounds but THC (32%-36%, which was found to be acceptable).…”

Section: Discussionmentioning

confidence: 99%

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Simultaneous Screening and Quantification of 29 Drugs of Abuse in Oral Fluid by Solid-Phase Extraction and Ultraperformance LC-MS/MS

et al. 2009

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Background: The European DRUID (Driving under the Influence of Drugs, Alcohol And Medicines) project calls for analysis of oral fluid (OF) samples, collected randomly and anonymously at the roadside from drivers in Denmark throughout 2008–2009. To analyze these samples we developed an ultra performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method for detection of 29 drugs and illicit compounds in OF. The drugs detected were opioids, amphetamines, cocaine, benzodiazepines, and Δ-9-tetrahydrocannabinol. Method: Solid-phase extraction was performed with a Gilson ASPEC XL4 system equipped with Bond Elut Certify sample cartridges. OF samples (200 mg) diluted with 5 mL of ammonium acetate/methanol (vol/vol 90:10) buffer were applied to the columns and eluted with 3 mL of acetonitrile with aqueous ammonium hydroxide. Target drugs were quantified by use of a Waters ACQUITY UPLC system coupled to a Waters Quattro Premier XE triple quadrupole (positive electrospray ionization mode, multiple reaction monitoring mode). Results: Extraction recoveries were 36%–114% for all analytes, including Δ-9-tetrahydrocannabinol and benzoylecgonine. The lower limit of quantification was 0.5 μg/kg for all analytes. Total imprecision (CV) was 5.9%–19.4%. With the use of deuterated internal standards for most compounds, the performance of the method was not influenced by matrix effects. A preliminary account of OF samples collected at the roadside showed the presence of amphetamine, cocaine, codeine, Δ-9-tetrahydrocannabinol, tramadol, and zopiclone. Conclusions: The UPLC-MS/MS method makes it possible to detect all 29 analytes in 1 chromatographic run (15 min), including Δ-9-tetrahydrocannabinol and benzoylecgonine, which previously have been difficult to incorporate into multicomponent methods.

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“…Certains auteurs l'utilisent également pour les cheveux [28,29,32] ou le fluide oral [41,[62][63][64].…”

Section: Extraction Sur Phase Solideunclassified

Analyse des cannabinoïdes par spectrométrie de masse en mode tandem

Fabritius¹,

Staub²,

Giroud³

2011

Ann Toxicol Anal

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Résumé -Depuis quelques années, la spectrométrie de masse en tandem (MS/MS) ne cesse de gagner du terrain comme méthode d'analyse en toxicologie forensique, notamment pour le dosage des cannabinoïdes. Couplée à la chromatographie liquide (LC) ou gazeuse (GC), elle permet l'identification fiable et le dosage rapide du THC, de son précurseur acide, et de ses principaux métabolites, y compris les glucuronides. Au cours de ces dix dernières années, un nombre significatif de publications sont parues sur ce sujet. L'objectif de cet article est de passer en revue les analyses par spectrométrie de masse en tandem des cannabinoïdes dans diverses matrices biologiques.Mots clés : Cannabinoïdes, spectrométrie de masse en tandem, matrice biologique, chromatographie Abstract -In recent years, tandem mass spectrometry (MS/MS) is gaining ground as a reference method of analysis in clinical and forensic toxicology, especially for the determination of cannabinoids. Coupled to liquid chromatography (LC) or gas chromatography (GC), it allows the definitive identification and rapid determination of THC, its acid precursor, and its major metabolites, including the glucuronides. During the past decade, several methods of analysis of cannabinoids in different matrices have appeared on this subject. The aim of this paper is to review the analysis of cannabinoids by tandem mass spectrometry methods in various biological matrices.

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Determination of illicit and medicinal drugs and their metabolites in oral fluid and preserved oral fluid by liquid chromatography–tandem mass spectrometry

Cited by 94 publications

References 12 publications

Alcohol and drug use by Spanish drivers: Comparison of two cross-sectional road-side surveys (2008–9/2013)

Alcohol and drug use by Spanish drivers: Comparison of two cross-sectional road-side surveys (2008–9/2013)

Simultaneous Screening and Quantification of 29 Drugs of Abuse in Oral Fluid by Solid-Phase Extraction and Ultraperformance LC-MS/MS

Analyse des cannabinoïdes par spectrométrie de masse en mode tandem

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